State Key Laboratory for Biology of Plant Diseases and Insect Pests

Beijing, China

State Key Laboratory for Biology of Plant Diseases and Insect Pests

Beijing, China
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Ren J.,State Key Laboratory for Biology of Plant Diseases and Insect Pests | Tao C.,Chinese Institute for the Control of Agrochemicals | Zhang L.,Chinese Institute for the Control of Agrochemicals | Ning J.,State Key Laboratory for Biology of Plant Diseases and Insect Pests | And 2 more authors.
Pest Management Science | Year: 2017

BACKGROUND: Treated soil is the second most prevalent application technique for all registered pesticides in China. Some developing countries also adopt this method. However, the safety of this scenario has not been reported in the literature. Experiments were therefore conducted to assess exposure using standard whole-body dosimetry and air sampling methodologies. RESULTS: Dermal deposition was the main route of exposure in this scenario. The total dermal unit exposure (UE) of operators to clothianidin-treated soil was 51.7 mg kg-1 AI handled (SD = 20.59, n = 16), and hands accounted for 36%. Inhalation UE was 0.04 mg kg-1 AI handled (SD = 0.02, n = 4), negligible compared with dermal exposure. Using an NOAEL (no observed adverse effect level) of 10 mg kg-1 day-1, the margin of exposure was 773, i.e. greater than 100. CONCLUSION: For the first time, the scenario of treated soil exposure was assessed and was found to pose less risk than conventional pesticide application. These results can be used as a reference in pesticide management. © 2017 Society of Chemical Industry.

Sun L.,Tea Research InstituteChinese Academy of Agricultural SciencesHangzhou China | Wang Q.,Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing China | Yang S.,Key Laboratory of AgroBiotechnology | Wang Q.,State Key Laboratory for Biology of Plant Diseases and Insect Pests | And 4 more authors.
Insect Molecular Biology | Year: 2017

The polyphagous mirid bug Adelphocoris lineolatus relies heavily on olfactory cues to track suitable host plants. Thus, a better understanding of the molecular basis of its olfactory detection could contribute to the development of effective pest management strategies. In the present study, we report the expression profile of the odorant binding protein gene A. lineolatus odorant binding protein 6 (AlinOBP6). Quantitative real-time PCR experiments suggest that AlinOBP6 is female adult antennae-biased. Cellular immunolocalization analyses show that AlinOBP6 is highly expressed in the lymph of both multiporous sensilla basiconica and uniporous sensilla chaetica. A ligand binding analysis showed that recombinant AlinOBP6 not only bound tightly to host plant volatile compounds but also to nonvolatile compounds. Homology modelling and molecular docking analyses confirmed these unusual ligand binding profiles and revealed that the amino acid residues involved in the recognition of volatile and nonvolatile compounds are distinct. The results of our study are the first to suggest that an antenna- and female-biased OBP in an hemipteran insect is expressed in both olfactory and gustatory sensilla as a mechanism to respond to volatile and nonvolatile host compounds. These findings warrant further research into the molecular mechanisms of chemosensation for mirid bugs in responsive to host plant location. © 2017 The Royal Entomological Society.

Yu C.,State Key Laboratory for Biology of Plant Diseases and Insect Pests | Chen H.,State Key Laboratory for Biology of Plant Diseases and Insect Pests | Tian F.,State Key Laboratory for Biology of Plant Diseases and Insect Pests | Yang F.,State Key Laboratory for Biology of Plant Diseases and Insect Pests | He C.,State Key Laboratory for Biology of Plant Diseases and Insect Pests
Molecular Plant Pathology | Year: 2017

Flagellin glycosylation plays a crucial role in flagellar assembly, motility and virulence in several pathogenic bacteria. However, little is known about the genetic determinants and biological functions of flagellin glycosylation in Xanthomonas oryzae pv. oryzae (Xoo), the causal pathogen of bacterial blight of rice. Here, the structure, regulation and functions of a ten-gene cluster gigX (glycosylation island genes of Xoo), which was embedded in a flagellar regulon, were characterized. gigX1 to gigX10 encoded putative enzymes or proteins involved in glycan biosynthesis and transfer, including a nucleotide sugar transaminase, an acyl-carrier protein (ACP), a 3-oxoacyl-ACP synthase, a 3-oxoacyl-ACP reductase, a dehydrogenase, an acetyltransferase, a ring hydroxylating dioxygenase, a hypothetical protein, a methyltransferanse and a glycosyltransferase, respectively. The gigX genes were co-transcribed in an operon and up-regulated by the upstream σ54 factor RpoN2 and transcriptional activator FleQ. In-frame deletion of each gigX gene affected flagellin glycosylation modification, meaning that the unglycosylated flagellin of the mutants was smaller than the glycosylated flagellin of the wild-type. No significant changes in flagellar filament and motility were observed in the ΔgigX mutants, among which only ΔgigX6 displayed increased swimming ability. Importantly, all mutants, except ΔgigX9, showed significantly increased virulence and bacterial growth in the susceptible rice cultivar IR24, and ΔgigX1 and ΔgigX10 showed enhanced type III secretion system (T3SS)-related gene expression. Moreover, the glycosylated flagellin of the wild-type induced higher H2O2 levels in rice leaves than did the unglycosylated flagellins of ΔgigX1 or ΔgigX10. Taken together, this study reveals that the gigX cluster determines flagellin glycosylation, and implicates the regulatory role of post-translational modification with the glycosylation, acetylation and methylation of flagellin in the regulation of motility and virulence of Xoo. © 2017 BSPP AND JOHN WILEY & SONS LTD.

Zhong X.,State Key Laboratory for Biology of Plant Diseases and Insect Pests | Yang J.,State Key Laboratory for Biology of Plant Diseases and Insect Pests | Shi Y.,State Key Laboratory for Biology of Plant Diseases and Insect Pests | Wang X.,State Key Laboratory for Biology of Plant Diseases and Insect Pests | Wang G.-L.,Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing100193 China
Molecular Plant Pathology | Year: 2017

Rice blast, caused by Magnaporthe oryzae (synonym: Pyricularia oryzae), severely reduces rice production and grain quality. The molecular mechanism of rice resistance to M. oryzae is not fully understood. In this study, we identified a chaperone DnaJ protein, OsDjA6, which is involved in basal resistance to M. oryzae in rice. The OsDjA6 protein is distributed in the entire rice cell. The expression of OsDjA6 is significantly induced in rice after infection with a compatible isolate. Silencing of OsDjA6 in transgenic rice enhances resistance to M. oryzae and also results in an increased burst of reactive oxygen species after flg22 and chitin treatments. In addition, the expression levels of WRKY45, NPR1 and PR5 are increased in OsDjA6 RNAi plants, indicating that OsDjA6 may mediate resistance by affecting the salicylic acid pathway. Finally, we found that OsDjA6 interacts directly with the E3 ligase OsZFP1 in vitro and in vivo. These results suggest that the DnaJ protein OsDjA6 negatively regulates rice innate immunity, probably via the ubiquitination proteasome degradation pathway. © 2017 BSPP AND JOHN WILEY & SONS LTD.

Sun L.,State Key Laboratory for Biology of Plant Diseases and Insect Pests | Sun L.,Chinese Academy of Agricultural Sciences | Qiu G.,State Key Laboratory for Biology of Plant Diseases and Insect Pests | Qiu G.,Chinese Academy of Agricultural Sciences | And 4 more authors.
Pesticide Biochemistry and Physiology | Year: 2015

Chlorantraniliprole is a novel diamide insecticide that targets the insect ryanodine receptor, a Ca2+ release channel. Spodoptera exigua is a significant insect pest, and chlorantraniliprole is the most popular diamide insecticide used against this pest. To better understand the effects of diamides on RyR expression and [Ca2+], we isolated the SeRyR cDNA and investigated changes in SeRyR expression as a result of the application of chlorantraniliprole. The full-length cDNAs of SeRyR contain an open reading frame (ORF) of 15,357 bp with a predicted protein consisting of 5118 amino acids. SeRyR shares 77-92% identity with other insect RyR isoforms and 45-47% identity with vertebrate RyR isoforms. Furthermore, the relative expression abundances of RyR mRNA extracted from S. exigua fat body cells after 24 h of culture in 0.1, 1, 10, 100 nM, 1 μM and 100 μM of chlorantraniliprole changed 1.04-, 0.89-, 1.83-, 2.58-, 4.03- and 3.12-fold compared to blank control, respectively. The regression equation for the relative expression levels of SeRyR after 24 h as a function of the chlorantraniliprole concentration was Y = 0.6455 + 0.8188LgX, R2 = 0.97093 for the cell line IOZCAS-Spex-II. These results outline the effects of chlorantraniliprole on the expression of SeRyR and provide a basis for the discovery of a compound that may exhibit selective insect activity. © 2015 Elsevier Inc.

Wang H.-Z.,South China Agricultural University | Wang H.-Z.,Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms | Chu Z.-Z.,South China Agricultural University | Chu Z.-Z.,Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms | And 18 more authors.
PLoS ONE | Year: 2015

Fusion tag is one of the best available tools to date for enhancement of the solubility or improvement of the expression level of recombinant proteins in Escherichia coli. Typically, two consecutive affinity purification steps are often necessitated for the purification of passenger proteins. As a fusion tag, acyl carrier protein (ACP) could greatly increase the soluble expression level of Glucokinase (GlcK), α-Amylase (Amy) and GFP. When fusion protein ACP-G2-GlcK-Histag and ACP-G2-Amy-Histag, in which a protease TEV recognition site was inserted between the fusion tag and passenger protein, were coexpressed with protease TEV respectively in E. coli, the efficient intracellular processing of fusion proteins was achieved. The resulting passenger protein GlcK-Histag and Amy-Histag accumulated predominantly in a soluble form, and could be conveniently purified by one-step Nichelating chromatography. However, the fusion protein ACP-GFP-Histag was processed incompletely by the protease TEV coexpressed in vivo, and a large portion of the resulting target protein GFP-Histag aggregated in insoluble form, indicating that the intracellular processing may affect the solubility of cleaved passenger protein. In this context, the soluble fusion protein ACP-GFP-Histag, contained in the supernatant of E. coli cell lysate, was directly subjected to cleavage in vitro by mixing it with the clarified cell lysate of E. coli overexpressing protease TEV. Consequently, the resulting target protein GFP-Histag could accumulate predominantly in a soluble form, and be purified conveniently by one-step Nichelating chromatography. The approaches presented here greatly simplify the purification process of passenger proteins, and eliminate the use of large amounts of pure site-specific proteases. © 2015 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Liusha H.,Agricultural University of Hebei | Zaifeng L.,Agricultural University of Hebei | Xiaodong W.,Agricultural University of Hebei | Zhanhai K.,Agricultural University of Hebei | And 6 more authors.
Pakistan Journal of Botany | Year: 2016

Chinese wheat cultivar “Lantian 9” showed a stable high yield in the field. Together with its other desirable traits such as tolerance to cold, drought, leaf rust and stripe rust, made it a good source for wheat breading. In our preliminary test, “Lantian 9” showed a typical adult resistance phenotype (susceptible at seedling stage but high resistance at adult stage) to most of the major Chinese leaf rust pathotypes. To clarify the adult-plant resistance (APR) genes in this cultivar, a population with 197 F2:3 lines was generated by crossing “Lantian 9” with susceptible line “Huixian Hong”. The population was phenotyped in the field over three years (year 2012, 2013 and 2014) by a mixture of three leaf rust pathotypes (THTT, THTS and THTQ). A total of 1232 simple sequence repeat (SSR) markers were used to screen the parental lines. Polymorphic ones were further applied on the population. Linkage mapping analysis showed that one QTL from “Lantian 9” was located on chromosome 2BS, which was relative stable among the data from the year 2012 and 2013 with phenotypic variations of 6.0% and 9.1%, respectively. Three other QTLs from “Lantian 9” on chromosome 4BS, 3A and 1BL were detected. We also identified one QTL from “Huixian Hong” on chromosome 1BL. All these identified wheat leaf rust resistance QTLs with their closely linked molecular markers will greatly facilitate genetic improvement of wheat resistance to leaf rust in China. © 2016, Pakistan Botanical Society. All Rights reserved.

Luo J.,Huazhong Agricultural University | Liu X.,Huazhong Agricultural University | Liu L.,Huazhong Agricultural University | Zhang P.,Huazhong Agricultural University | And 5 more authors.
Gene | Year: 2014

Background: Adelphocoris suturalis Jakovlev is a major cotton pest in Southern China. Metathoracic scent glands (MTGs) produced pheromones that play an important role in survival and population propagation of this species, and also show great potential for pest control. Up to the present, there is little information that underlined the molecular basis of the pheromone biosynthesis of this bug. It is essential to clarify genes involved in the production of pheromone components, and also in the regulation of the variation of the blend ratio. Results: We sequenced the transcriptome of metathoracic scent glands (MTGs) of A. suturalis. A total of 52 million 91-bp-long reads were obtained and assembled into 70,296 unigenes with a mean length of 691bp. Of these unigenes, a total of 26,744 (38%) unigenes showed significant similarity to known proteins in the NCBI database (E-value<10-5). Out of 26,744 hits, 9258 sequences were classified functionally into 25 COG categories, 16,473 unigenes were assigned to 242 KEGG pathways. Through blast searches of public database, a series of transcripts encoding proteins potentially related to the pheromone biosynthesis were selected, and the gene expression patterns were verified by qRT-PCR. The qRT-PCR results indicated that Asdelta9-DES, AsFAR, AsAOX, Ascarboxylesterase, AsNT-ES and AsATFs have a higher expression level in the period when female A. suturalis release sex pheromones. Conclusions: These data constitutes the first transcriptomic analysis exploring the repertoire of genes expressed in insect MTGs. We identified a large number of potential pheromone biosynthetic pathway genes. In this context, our study provides an invaluable resource for future exploration of molecular mechanisms of pheromone biosynthesis in A. suturalis, as well as other hemipteran species. © 2014 Elsevier B.V.All rights reserved.

Qiao H.,Henan University | Qiao H.,State Key Laboratory for Biology of Plant Diseases and Insect Pests | Shi Y.,Henan University | Si H.,Henan University | And 5 more authors.
Nongye Gongcheng Xuebao/Transactions of the Chinese Society of Agricultural Engineering | Year: 2014

Wheat take-all is a quarantine disease, which will lead to a disaster in wheat production without timely monitoring and management. Remote sensing technique, especially the field-based imaging spectrum technique, can achieve real-time monitoring of the disease development. For rapid extraction of take-all disease information, we try to monitor wheat take-all disease using imaging spectrometer. The experiment was carried out in Baisha village, Yuanyang County of China. We designed test of three concentration gradients and repeated three times, the experimental field was 30 m2. The wheat take-all white head rate was surveyed two weeks before harvest. The wheat's canopy spectrum was collected by two kinds of spectrometer, ASD Handheld non-imaging spectrometer (ASD Handheld, ASD Inc.) and Headwall imaging spectrometer (HyperSpec® VNIR, Headwall Photonics, Inc.). All data were collected between 10:00 to 13:00 in sunny days. In this study, based on gray association analysis (GAA) and support vector machine (SVM) classifier, a spectral feature extraction and classification method was proposed to separate the spectral features of the different take-all levels from spectral images. The field-based spectral images were acquired by Headwall imaging sensor. Meanwhile, the spectral data about different white head rate were collected by ASD HandHeld non-imaging sensor. Because of better accuracy and resolution, ASD spectral data had a better capacity to express the spectral features of take-all levels. These spectral features were extracted using kernel principle component analysis (K-PCA). Characteristic bands of the first four of principal component was mainly green band, red band and near infrared band, indicated in the spectrum curve, peak and valley phenomenon was the main distinguishing feature of white head rate and take-all disease grade. Then Jeffries-Matusita distances between feature bands were calculated, if Jeffries-Matusita distances between feature bands were greater than 1.8, the selected characteristic bands can distinguish different damage degree of wheat take-all disease. The spectral separability of take-all levels was tested and assessed by grey association analysis. Based on these significant features, some of Headwall imaging spectral data with different take-all levels were selected as the training data for the field-based spectral images. Through the SVM classifier based on RBF kernel function, a hyperspectral classification image of take-all was calculated. Results showed that the wheat take-all widely existed in the experimental zone, but its distribution had own specific characteristic with different disease levels. The slight disease wheat and the heavy disease wheat were mixture in the experimental zone. The distribution characteristics of serious take-all wheat disease (white head rate greater than 60%) were intensive and block. Slight wheat disease (white head rate between 10%-30%) were widely distributed in the middle of heavy wheat disease(white ear rate between 30%-60%), the proportion of slight wheat disease and heavy heat disease was 29.53% and 26.06%, respectively, very serious wheat take-all disease (white head rate between 60%-90%) and death of wheat disease showed regional distribution in the image, accounted for 10.73% and 19.91%.The overall accuracy of the classification was greater than 94% (Kappa>0.8). To further validate the classification accuracy, field experiment survey data was compared with the spectral classification, misclassification existed mainly in white head rate 30%~40%.These results proves the field-based imaging spectrum has the capacity to achieve the real-time monitoring and classification of wheat take-all condition, and to support the guidance on wheat production. ©, 2014, Chinese Society of Agricultural Engineering. All right reserved.

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