State Key Laboratory for Agro Biotechnology

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Yan J.,Chinese Academy of Sciences | Yan H.,State Key Laboratory for Agro Biotechnology | Yan H.,China Agricultural University | Liu L.X.,State Key Laboratory for Agro Biotechnology | And 7 more authors.
Archives of Microbiology | Year: 2016

One Gram-negative, aerobic, motile, rod-shaped bacterium, designated as FH14T, was isolated from nodules of Phaseolus vulgaris grown in Hidalgo State of Mexico. Results based upon 16S rRNA gene (≥99.8 % similarities to known species), concatenated sequence (recA, atpD and glnII) analysis of three housekeeping genes (≤93.4 % similarities to known species) and average nucleotide identity (ANI) values of genome sequence (ranged from 87.6 to 90.0 % to related species) indicated the distinct position of strain FH14T within the genus Rhizobium. In analyses of symbiotic genes, only nitrogen fixation gene nifH was amplified that had nucleotide sequence identical to those of the bean-nodulating strains in R. phaseoli and R. vallis, while nodulation gene nodC gene was not amplified. The failure of nodulation to its original host P. vulgaris and other legumes evidenced the loss of its nodulation capability. Strain FH14T contained summed feature 8 (C18:1ω6c/C18:1ω7c, 59.96 %), C16:0 (10.6 %) and summed feature 2 (C12:0 aldehyde/unknown 10.928, 10.24 %) as the major components of cellular fatty acids. Failure to utilize alaninamide, and utilizing l-alanine, l-asparagine and γ-amino butyric acid as carbon source, distinguished the strain FH14T from the type strains for the related species. The genome size and DNA G+C content of FH14T were 6.94 Mbp and 60.8 mol %, respectively. Based on those results, a novel specie in Rhizobium, named Rhizobium hidalgonense sp. nov., was proposed, with FH14T (=HAMBI 3636T = LMG 29288T) as the type strain. © 2016 Springer-Verlag Berlin Heidelberg


Yao Y.,State Key Laboratory for Agro Biotechnology | Sui X.H.,State Key Laboratory for Agro Biotechnology | Zhang X.X.,Chinese Academy of Agricultural Sciences | Wang E.T.,State Key Laboratory for Agro Biotechnology | Chen W.X.,State Key Laboratory for Agro Biotechnology
International Journal of Systematic and Evolutionary Microbiology | Year: 2015

Six slow-growing rhizobial strains isolated from effective nodules of Erythrophleum fordii were classified into the genus Bradyrhizobium based on their 16S rRNA gene sequences. The results of multilocus sequence analysis of recA, glnII and gyrB genes and 16S–23S rRNA intergenic spacer (IGS) sequence phylogeny indicated that the six strains belonged to two novel species, represented by CCBAU 53325T and CCBAU 51502T, which were consistent with the results of DNA–DNA hybridization; CCBAU 53325T had 17.65–25.59% relatedness and CCBAU 51502T had 22.69–44.58% relatedness with five closely related type strains, Bradyrhizobium elkanii USDA 76T, B. pachyrhizi LMG 24246T, B. lablabi CCBAU 23086T, B. jicamae LMG 24556T and B. japonicum USDA 6T. In addition, analysis of phenotypic characteristics and fatty acid profiles also distinguished the test strains from defined species of Bradyrhizobium. Two novel species, Bradyrhizobium erythrophlei sp. nov., represented by the type strain CCBAU 53325T (=HAMBI 3614T=CGMCC 1.13002T=LMG 28425T), and Bradyrhizobium ferriligni sp. nov., represented by the type strain CCBAU 51502T (=HAMBI 3613T=CGMCC 1.13001T), are proposed to accommodate the strains. © 2015, IUMS.


PubMed | Chinese Academy of Sciences, State Key Laboratory for Agro Biotechnology, National Polytechnic Institute of Mexico and Chinese Academy of Agricultural Sciences
Type: | Journal: Archives of microbiology | Year: 2016

One Gram-negative, aerobic, motile, rod-shaped bacterium, designated as FH14

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