The State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST

Wuhan, China

The State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST

Wuhan, China
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Zeng M.,The State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST | Yang S.-D.,Hubei University | Zhang J.-L.,Hubei University | Chen X.-M.,Hubei University
Oncology Letters | Year: 2015

Small cell neuroendocrine carcinoma (SNEC) of the oral cavity is a rare and distinctive tumor with aggressive clinical behavior. Thus far, only a small number of cases have been reported and no definitive standard treatment strategy has been determined. The current study reports a case of oral SNEC arising in the lower gingiva in a 73-year-old male. Computed tomography displayed a relatively well-defined mass measuring 2.8x2x1.4 cm in size. The mass was located in the buccal side of the right mandibular posterior gingiva and exhibited no bony involvement. Histopathological examination revealed a proliferation of small cells with ovoid- to spindle-shaped nuclei, fine granular chromatin, inconspicuous nucleoli, scant cytoplasm and high mitotic activity. Immunohistochemically, the tumor cells were positive for cytokeratin AE1/AE3, chromogranin A, synaptophysin and neuron-specific enolase. Surgical resection and radical neck dissection were performed prior to the administration of adjuvant chemotherapy with a combination of cisplatin and etoposide. No evidence of local recurrence or metastasis was observed at 14 months post-surgery. © 2015, Spandidos Publications. All rights reserved.


Sun Z.-J.,The State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST | Sun Z.-J.,Wuhan University | Cai Y.,The State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST | Cai Y.,Wuhan University | And 9 more authors.
Histopathology | Year: 2010

Aims: Infantile haemangiomas (IHs) are common benign vascular tumours distinctive for their perinatal presentation, rapid growth during the first year of life and subsequent slow involution. Recent research has indicated that endothelial cells of haemangiomas express LIM-only protein 2 (LMO2). The aim of this study was to investigate the role of LMO2 in the pathogenesis of IHs was investigated.Methods and results: Immunoreactivity of LMO2 was assessed in specimens of 19 IH. Stable transfection of LMO2 into human endothelial cell lines (EAhy926) was performed to evaluate the role of LMO2 in terms of the change in cell proliferation, cell cycle and cell migration as well as the expression level of angiogenic factors. Immunoreactivity for LMO2 was detected in all IH specimens, specifically in the nucleus of the endothelial cells. The intensity of LMO2 immunostaining decreased significantly from proliferative to involuting stages. Furthermore, the overexpression of LMO2 enhanced the proliferation and migration of the endothelial cells and promoted G0/G1-S-phase transition in vitro, together with an up-regulation of bFGF expression.Conclusions: LMO2 probably promotes angiogenesis by up-regulation of bFGF expression and thereby consequently influences progression of IH. © 2010 Blackwell Publishing Limited.


Liu J.,The State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST | Liu J.,Inner Mongolia University | Wang Y.,Inner Mongolia University | He H.,The State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST | And 3 more authors.
Experimental and Therapeutic Medicine | Year: 2015

Salivary gland malignant neoplasms (SGMNs) represent a group of malignant solid tumors with heterogeneity in their cellular make-up, which causes difficulty with regard to the immunohistochemical confirmation of their cytological features. In the present study, overexpression of the pituitary tumor transforming gene (PTTG) was evaluated in human mucoepidermoid carcinoma specimens with a submaxillary salivary gland origin by immunohistochemical analysis, western blot analysis and reverse transcription quantitative polymerase chain reaction. In addition, a SGMN cell line was constructed, namely A-253 PTTG (+), which overexpressed PTTG. Subsequently, the regulatory role of PTTG in the proliferation and migration of A-253 cells was investigated. The immunohistochemical results demonstrated that there was a higher rate of PTTG-positive cells in the SGMN tissues when compared with the control submaxillary salivary gland tissues. Furthermore, PTTG expression at a mRNA and protein level was significantly higher in the SGMN specimens when compared with the control specimens. In addition, the rates of proliferation and migration of the A-253 PTTG (+) cells were significantly higher compared with the A-253 PTTG (-) cells. Therefore, PTTG was demonstrated to play an important role in SGMN cell proliferation and migration, and may subsequently be a notable marker for SGMN diagnosis and a potential target for anticancer therapy. © 2015, Spandidos Publications. All rights reserved.

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