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Li Z.,Emory University | Li Z.,Georgia Regents University | Li Z.,State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST | Wang H.,Emory University | And 3 more authors.
Radiation Research | Year: 2014

Previous work has shown that high charge and energy particle irradiation of human cells evokes a mutagenic repair phenotype, defined by increased mutagenic repair of new double-strand breaks that are introduced enzymatically, days or weeks after the initial irradiation. The effect was seen originally with 600 MeV/u 56Fe particles, which have a linear energy transfer (LET) value of 174 keV/μm, but not with X rays or γ rays (LET ≤ 2 keV/μm). To better define the radiation quality dependence of the phenomenon, we tested two ions with intermediate LET values, 1,000 MeV/u 48Ti (LET = 108 keV/μm) and 300 MeV/u 28Si (LET = 69 keV/μm). These experiments used a previously validated assay, where a rare-cutting nuclease introduces double-strand breaks in two reporter transgene cassettes, which are located on different chromosomes. Deletions of a block of sequence in one of the cassettes, or translocations between cassettes, are measured independently using a multicolor fluorescence assay. The results showed that 48Ti was a potent, but transient, inducer of mutagenic repair, based on increased frequency of nuclease-induced translocations. The 48Ti ions did not affect the frequency of nuclease-induced deletions. The 28Si ions had no measurable effect on either endpoint. There was a close correlation between the induction of the mutagenic repair phenomenon and the frequency of micronuclei in the targeted population (R2 = 0.74), whereas there was no apparent correlation with radiation-induced cell inactivation. Together, these results better define the radiation quality dependence of the mutagenic repair phenomenon and establish its correlation, or lack of correlation, with other endpoints. © 2014 by Radiation Research Society. Source


Li B.,Hubei University | Li B.,Hubei University of Education | Long X.,Hubei University of Education | Long X.,State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST | And 3 more authors.
Journal of Oral and Maxillofacial Surgery | Year: 2011

Purpose To evaluate the clinical and radiologic features of desmoplastic ameloblastoma (DA). Materials and Methods Twenty-four cases of DA among Chinese patients were analyzed retrospectively. The clinical and radiologic features of DA at present, as reported in the English-language literature, were reviewed and its radiologic characteristics critically evaluated. Results This study included 23 cases of intraosseous lesions and 1 case of extraosseous lesion. Clinically, DA is mainly located in the anterior and/or the premolar region of the jaw, with a slight male prominence. Among 23 intraosseous lesions, 9 cases occurred in the maxilla and 14 occurred in the mandible. Radiographically, 69.6% (16 of 23) exhibited ill-defined borders. Root displacement occurred in 47.9% of patients (11 of 23) and root resorption occurred in 8.7% of patients (2 of 23). Three radiologic presentations of DA were identified as follows: type I, which is both radiolucent and radiopaque in appearance (osteofibrosis type); type II, which has a completely radiolucent appearance (radiolucent type); and type III, which is both radiolucent and radiopaque in appearance combined with a large radiolucent change (compound type). The osteofibrosis type (14 cases) is the most common pattern, and the compound type is the least common (only 3 cases). Conclusions DA has a potentially aggressive behavior. Moreover, it exhibits 3 different radiologic characteristics. Although DA should be differentiated from osteofibrosis lesions frequently described in the literature, some uncommon radiographic patterns should be considered in the differential diagnosis of this rare odontogenic tumor. © 2011 American Association of Oral and Maxillofacial Surgeons. Source


Jiang S.-J.,State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST | Li W.,State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST | Li Y.-J.,State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST | Fang W.,Hubei University | Long X.,Hubei University
Molecular Medicine Reports | Year: 2015

Angiogenesis has an important role in the progression of temporomandibular joint disorders (TMD). The aim of the present study was to explore the association between dickkopf-related protein 1 (DKK-1) and angiogenesis in TMD. The expression levels of DKK-1 and vascular endothelial growth factor (VEGF) were quantified by an ELISA assay of the synovial fluid from patients with TMD. The correlation between DKK-1 and VEGF was analyzed by Pearson correlation test. Synovial fibroblasts were isolated from patients with TMD and were subsequently treated with recombinant human DKK-1, anti-DKK-1 antibody, hypoxia inducible factor-1α (HIF-1α), or small interfering RNA (siRNA). The expression levels of DKK-1, HIF-1α, and VEGF were subsequently quantified. The present study also investigated the effects of DKK-1 on the migration of human umbilical vein endothelial cells (HUVEC). Increased expression levels of DKK-1 were concordant with increased expression levels of VEGF in the synovial fluid from patients with TMD. In the synovial fibroblasts, DKK-1 increased the expression levels of VEGF, and promoted HIF-1α nuclear localization. In addition, DKK-1 induced HUVEC migration, and HIF-1α siRNA inhibited DKK-1-induced cell migration. The results of the present study indicate that DKK-1 is associated with angiogenesis in the synovial fluid of patients with TMD. Furthermore, HIF-1α may be associated with DKK-1-induced HUVEC activation. Source


Zhu X.,State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST | Zhang J.,State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST | Zhang J.,Wuhan University | Chen X.,Wuhan University | Feng X.,Hebei United University
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology | Year: 2012

Comparison of Ki-67, cyclin E, and p63 in benign and malignant human pleomorphic adenoma Xiaojie Zhu, DDS,a Jiali Zhang, DDS, PhD,a,b Xinming Chen, DDS,b and Xiaojie Feng, DDSc Wuhan University, Wuhan, China; and Hebei United University, Tangshan, China Objective. A set of cell proliferation-related molecular markers was used to detect the differences in proliferative activity between benign pleomorphic adenoma (PA) and carcinoma ex pleomorphic adenoma (CXPA). Study Design. Seventy samples comprising 53 PAs, 10 noninvasive and 7 widely invasive (WI) CXPAs were selected. Immunohistochemical staining was used to detect Ki-67, cyclin E, and p63 expression. Results. The average Ki-67 labeling index of WI CXPAs was higher than that of PAs and the malignant component of noninvasive CXPAs (M-noninvasive CXPA; P < .01). An increased cyclin E-positive staning was observed in M-noninvasive CXPAs and WI CXPAs compared with PAs (P = .006). The p63 expression levels were notably decreased in M-noninvasive CXPAs and WI CXPAs compared with PAs (P = .007). Conclusions. The data support that increased or decreased expression of Ki-67, cyclin E, and p63 may indicate the proliferative differences between PA and CXPA. © 2012 Elsevier Inc. All rights reserved. Source


Wang P.,State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST | Wei J.,State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST | Li Q.,State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST | Wang Y.,State Key Laboratory Breeding Base of Basic Science of Stomatology Hubei MOST
Journal of Prosthetic Dentistry | Year: 2014

Statement of problem. Color errors associated with current shade guides and problems with color selection and duplication are still challenging for restorative dentists.Purpose. The purpose of this study was to evaluate an optimized shade guide for visual shade duplication.Material and methods. Color distributions (L∗, a∗, and b∗) of the maxillary left central incisors of 236 participants, whose ages ranged from 20 to 60, were measured with a spectrophotometer. Based on this color map, an optimized shade guide was designed with 14 shade tabs evenly distributed within the given color range of the natural incisors. The shade tabs were fabricated with porcelain powder mixtures and conventional laboratory procedures. A comparison of shade duplication by using the optimized and Vitapan Classical shade guides was conducted. Thirty Chinese participants were involved, and the colors of the left maxillary incisors were selected by using 2 shade guides. Metal ceramic crowns were fabricated according to the results of the shade selection. The colors of the shade tabs, natural teeth, and the ceramic crowns were measured with a spectrophotometer. The color differences among the natural teeth, the shade tabs, and the corresponding metal ceramic crowns were calculated and analyzed (α=.017).Results. Significant differences were found in both phases of shade determination and shade duplication (P<.017). The total number of color errors with the optimized shade guide was 3.5, which was significantly less than that of Vitapan, 5.1 (P<.001).Conclusions. The optimized shade guide system improved performance not only in the color selection phase but also in the color of the fabricated crowns. © 2014 Editorial Council for the Journal of Prosthetic Dentistry. Source

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