Song L.,Wuhan University |
Song L.,Nanchang University |
Song L.,State Key Laboratory Breeding Base of Basic Science of Stomatology |
Li C.,Wuhan University |
And 10 more authors.
Photomedicine and Laser Surgery | Year: 2015
Objective: Photodynamic therapy (PDT) triggers various cellular responses and induces cell death via necrosis and/or apoptosis. This study evaluated the feasibility of using O2 and Ca2+ fluxes as indicators of apoptosis induced by rose bengal (RB)-mediated PDT in human oral squamous carcinoma cells (Cal27 cells). Methods: Intracellular reactive oxygen species (ROS) generation was assessed by the dichloro-dihydro-fluorescein diacetate (DCFH-DA) method. Real-time O2 and Ca2+ flux measurements were performed using the noninvasive micro-test technique (NMT). Apoptosis of the PDT-treated cells was confirmed by 4′6-diamidino-2-phenylindole-dilactate staining. The activation of apoptosis-related molecules was examined using Western blot. We assayed the effects of the fluctuation of O2 and Ca2+ flux in response to PDT and the apoptotic mechanism, by which ROS, O2, and Ca2+ synergistically may trigger apoptosis in PDT-treated cells. Results: Real-time O2 and Ca2+ flux measurements revealed that these indicators were involved in the timely regulation of apoptosis in the PDT-treated cells and were activated 2 h after PDT treatment. RB-mediated PDT significantly elicited the generation of ROS by approximately threefold, which was critical for PDT-induced apoptosis. Cytochrome c and cleaved caspase-3, caspase-9 and poly ADP ribose polymerase (PARP) were overexpressed, and the data provided evidence that 2 h was considered to be the key observation time in RB-mediated PDT-induced apoptosis in Cal27 cells. Conclusions: Our collective results indicated that the effects of O2 and Ca2+ fluxes may act as a real-time biomonitoring system of apoptosis in the RB-PDT-treated cells. Also, RB-mediated PDT can be a potential and effective therapeutic modality in oral squamous cell carcinoma. © 2015, Mary Ann Liebert, Inc.
Li Z.,State Key Laboratory Breeding Base of Basic Science of Stomatology |
Li Z.,Wuhan University |
Li Z.-B.,State Key Laboratory Breeding Base of Basic Science of Stomatology |
Li Z.-B.,Wuhan University
Journal of Oral and Maxillofacial Surgery | Year: 2012
Purpose: The purpose of this study was to evaluate mandibular condylar growth in growing rats after experimentally displaced condylar fracture with associated attachment damage and disc displacement by means of polychrome sequential labeling. Materials and Methods: We randomized 30 growing male Wistar rats into 3 equal groups: rats with experimentally displaced condylar fractures with associated attachment damage and disc displacement (experimental group); rats with experimentally displaced condylar fractures without associated attachment damage and disc displacement (control group); and rats that received no operative intervention (negative control group). Polychrome sequential labeling was used to establish chronologically oriented condylar growth characteristics in these rats. Three months after the operation, the specimens were obtained, fixed, dehydrated, and embedded in acrylic resin for fluorescence microscopy observation. In addition, the lateral-medial diameter (in millimeters) and mineral apposition rate (in micrometers per day) of condyles were measured and analyzed across the 3 groups. Results: The results showed condylar growth disturbance in the experimental group rats, presenting with no obvious and regular bone growth lines in the anamorphic condyle. However, in the control group and negative control group rats, the condylar growth was normal. Regarding the lateral-medial diameter and mineral apposition rate, there was a significant difference between the experimental group and control group, as well as between the experimental group and negative control group; however, there was no significant difference between the control group and negative control group. Conclusions: The occurrence of associated attachment damage and disc displacement in condylar fractures should be considered an important factor influencing the condylar growth after fracture. © 2012 American Association of Oral and Maxillofacial Surgeons.
Li Y.,State Key Laboratory Breeding Base of Basic Science of Stomatology |
El Mozen L.A.,Hubei University |
Cai H.,State Key Laboratory Breeding Base of Basic Science of Stomatology |
Fang W.,State Key Laboratory Breeding Base of Basic Science of Stomatology |
And 4 more authors.
Scientific Reports | Year: 2015
Synovial chondromatosis (SC) of temporomandibular joint is rare proliferative disorder featured by the formation of cartilaginous nodules in synovium and joint space. Transforming growth factor beta 3 (TGF-β23) is closely related to chondrogenic differentiation, and might participate in pathogenesis of SC. We discovered that increased quantity of synoviocytes and blood vessels were observed in SC synovium. The vessel wall and sublining fibroblasts were stained positively by the antibodies against TGF-β23, fibroblast growth factor 2 (FGF-2), and CD34. In loose bodies (LBs), TGF-β23 was mainly expressed in chondrocytes and FGF-2 was expressed in chondrocytes, fibroblasts, and vessel walls. Expressions of TGF-β21, TGF-β23, FGF-2, Sox9, Wnt-4, Foxc2, and VEGF-A mRNA were significantly higher in SC synovium. Stimulation of TGF-β23 on synoviocytes increased alkaline phosphatase (ALP) activity and expressions of chondrogenic genes (Sox9, Col2Î± 1, Aggrecan, Wnt-4, and Wnt-11), osteogenic genes (Runx2, Foxc2, osteocalcin, and Col1α1), and VEGF-A, but failed to influence FGF-2 expression. However, the addition of FGF-2 increased TGF-β23 expression. In conclusion, TGF-β23 existed in synovium and LBs of SC, and was responsible for the pathogenesis of SC.
Xu J.,State Key Laboratory Breeding Base of Basic Science of Stomatology |
Long X.,State Key Laboratory Breeding Base of Basic Science of Stomatology |
Cheng A.H.-A.,Royal Adelaide Hospital |
Cai H.,State Key Laboratory Breeding Base of Basic Science of Stomatology |
And 2 more authors.
Journal of Craniofacial Surgery | Year: 2015
Background: Temporomandibular joint (TMJ) ankylosis with facial asymmetry is still controversial to deal with. This study describes a modified condylar distraction osteogenesis protocol via preauricular approach for the treatment of this condition. Methods: From 2006 to 2013, 18 patients with TMJ ankylosis were enrolled. The Wuhan TMJ Ankylosis treatment protocol includes as follows: (1) preauricular approach is the only surgical access; (2) TMJ arthroplasty is used to recontour the condylar head, and the vertical height of condyle is maintained; (3) distractor placement with distractor port exiting via preauricular incision; (4) distraction after 5 to 7 days of latency period with 0.5 mm twice daily; and (5) distractor removal after 3-month consolidation through preauricular incision. All patients had clinical follow-up and detailed examination. Results: All patients had satisfactory results postoperatively. The mean (range) mouth opening increased from 7.1 (0-18) to 32.1 (28-43) mm during 37 (6-81) months of follow-up period (P < 0.01). Facial asymmetry was corrected in all patients, and all patients had minimal postoperative scar perception of the preauricular incision. Conclusions: TheWuhan TMJ ankylosis protocol provides a safe and effective treatment alternative in managing TMJ ankylosis, especially in young women who are anxious about perceptive extraoral scar. © 2015 Mutaz B. Habal, MD.