Entity

Time filter

Source Type


Naumoff D.G.,State Institute for Genetics and Selection of Industrial Microorganisms
Journal of Bioinformatics and Computational Biology | Year: 2010

The GH101 family is composed of endo-α-N-acetylgalactosaminidases and their homologues. Pairwise sequence comparison and phylogenetic analysis allowed us to distinguish five to six subfamilies in this family. Diverse domain structures were found among the family members. Usually they have five irreplaceable and some optional domains. Iterative screening of the protein database revealed an evolutionary relationship of the GH101 catalytic domain with glycoside hydrolase domains from GH13, GH31, and GH70 families. Among other homologous proteins we have found representatives of COG1649, as well as members of four new families of predicted glycoside hydrolases (GHL1-GHL4). © 2010 Imperial College Press.


Shalamitskiy M.Y.,State Institute for Genetics and Selection of Industrial Microorganisms | Naumov G.I.,State Institute for Genetics and Selection of Industrial Microorganisms
Russian Journal of Genetics | Year: 2016

Pectinase (endo-polygalacturonase) is the key enzyme splitting plant pectin. The corresponding single gene PGU1 is documented for the yeast S. cerevisiae. On the basis of phylogenetic analysis of the PGU nucleotide sequence available in the GenBank, a family of divergent PGU genes is found in the species complex S. bayanus: S. bayanus var. uvarum, S. eubayanus, and hybrid taxon S. pastorianus. The PGU genes have different chromosome localization. © 2016, Pleiades Publishing, Inc.


Naumov G.I.,State Institute for Genetics and Selection of Industrial Microorganisms | Shalamitskiy M.Y.,Russian Academy of Sciences | Naumova E.S.,State Institute for Genetics and Selection of Industrial Microorganisms
Doklady Biochemistry and Biophysics | Year: 2016

Using yeast genome databases and literature data, we have conducted a phylogenetic analysis of pectinase PGU genes from Saccharomyces strains assigned to the biological species S. arboricola, S. bayanus (var. uvarum), S. cariocanus, S. cerevisiae, S. kudriavzevii, S. mikatae, S. paradoxus, and hybrid taxon S. pastorianus (syn. S. carlsbergensis). Single PGU genes were observed in all Saccharomyces species, except S. bayanus. The superfamily of divergent PGU genes has been documented in S. bayanus var. uvarum for the first time. Chromosomal localization of new PGU1b, PGU2b, and PGU3b genes in the yeast S. bayanus var. uvarum has been determined by molecular karyotyping and Southern hybridization. © 2016, Pleiades Publishing, Ltd.


Naumov G.I.,State Institute for Genetics and Selection of Industrial Microorganisms | Naumova E.S.,State Institute for Genetics and Selection of Industrial Microorganisms
Russian Journal of Genetics | Year: 2011

Three different translocations involving chromosome IX have been detected in natural Saccharomyces cerevisiae strains using pulsed-field gel electrophoresis with intact chromosomal DNA and their hybridization with the SUC2 probe. Hybrids of these strains with genetic lines having normal molecular karyotype were shown to have back dislocation of at least marker SUC2 due to crossingover. The significance of the detected translocations is discussed. © 2011 Pleiades Publishing, Ltd.


Naumov G.I.,State Institute for Genetics and Selection of Industrial Microorganisms | Lee C.-F.,National Hsinchu University of Education | Naumova E.S.,State Institute for Genetics and Selection of Industrial Microorganisms
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | Year: 2013

Genetic hybridization, sequence and karyotypic analyses of natural Saccharomyces yeasts isolated in different regions of Taiwan revealed three biological species: Saccharomyces arboricola, Saccharomyces cerevisiae and Saccharomyces kudriavzevii. Intraspecies variability of the D1/D2 and ITS1 rDNA sequences was detected among S. cerevisiae and S. kudriavzevii isolates. According to molecular and genetic analyses, the cosmopolitan species S. cerevisiae and S. kudriavzevii contain local divergent populations in Taiwan, Malaysia and Japan. Six of the seven known Saccharomyces species are documented in East Asia: S. arboricola, S. bayanus, S. cerevisiae, S. kudriavzevii, S. mikatae, and S. paradoxus. © 2012 Springer Science+Business Media B.V.

Discover hidden collaborations