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Prague, Czech Republic

Vonka V.,Institute of Haematology and Blood Transfusion | Humlova Z.,Charles University | Klamova H.,Institute of Haematology and Blood Transfusion | Kujovska-Krcmova L.,University of Hradec Kralove | And 5 more authors.
OncoImmunology | Year: 2015

Indoleamine 2,3-dioxygenase 1 (IDO1), IDO2 and tryptophan 2,3-dioxygenase (TDO) represent some of the key immune regulators. Their increased activity has been demonstrated in a number of human malignancies but not yet in chronic myeloid leukemia (CML). In the present study, the activity of these enzymes was tested in 29 CML patients and 28 healthy subjects by monitoring the kynurenine (KYN)/tryptophan ratio. Serum samples taken prior to the therapy displayed a highly significant difference in KYN levels between the patient and control groups. However, increased KYN levels were detected in only 13 (44.8%) of these CML patients. The KYN levels in pretreatment sera of the patients correlated with the tumor burden. There was also a strong correlation between KYN levels and uric acid levels (UA). This suggests but does not prove the possible involvement of UA in activating IDO family of enzymes. Whenever tested, the increased KYN levels normalized in the course of the therapy. Patients with normal KYN levels in their pretreatment sera and subsequently treated with interferon-α, showed a transitory increase in their KYN levels. The present data indicate that CML should be added to the malignancies with an increased activity of the IDO family of enzymes and suggest that IDO inhibitors may be used in the treatment of CML patients. © 2015 Taylor & Francis Group, LLC.

Kozner P.,University Hospital Bulovka | Filous A.,Charles University | Jilich D.,Charles University | Brozek B.,University Hospital Bulovka | And 2 more authors.
Neuro-Ophthalmology | Year: 2011

The aim of the study was to evaluate the effect of human immunodeficiency virus infection on the retinal nerve fibre layer. The thickness was assessed in 48 human immunodeficiency virus-positive patients using scanning laser polarimetry. Results were compared to normal values and tested against factors suspected to affect the retinal nerve fibre layer. The mean value of the thickness was 57.65±6.18 μm in our cohort. No significant correlation between the retinal nerve fibre layer thickness and the immune profile or antiretroviral therapy was detected. However, a significant negative correlation between the retinal nerve fibre layer thickness with increasing length of human immunodeficiency virus infection was found. © 2011 Informa Healthcare USA, Inc.

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