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Miroshnikov S.A.,State Educational Institution All Russian Research Institute of Beef Cattle Breeding | Yausheva E.V.,State Educational Institution All Russian Research Institute of Beef Cattle Breeding | Sizova E.A.,Orenburg State University | Miroshnikova E.P.,Orenburg State University | Levahin V.I.,State Educational Institution All Russian Research Institute of Beef Cattle Breeding
Oriental Journal of Chemistry | Year: 2015

We studied the influence of highly dispersed copper particles on the metabolism of broiler chicks after a single intramuscular injection. Preparations containing of copper nanoparticles, agglomerates of nanoparticles of copper and copper microparticles were used in the experiment. Intramuscular injections of copper nanoparticles stimulate growth and metabolic changes quickly. Nanoparticle agglomerates and microparticles promote the similar effect but it is extended. The maximum difference in live weight was observed on the fourth day following injection of copper nanoparticles. The injection of copper nanoparticle agglomerates was accompanied by a significant increase in live weight 7 days after injection. The preparation containing copper microparticles had the smallest effect on growth. These changes were observed 16 days after microparticle injection. Copper nanoparticles a day after injection promoted increase in red cell level, hemoglobin, copper and protein in blood serum. Copper nanoparticle agglomerates and microparticles changed protein and copper content in blood serum only 7 and 21 days after injection. Copper microparticles had prolonged effect. The copper preparations promoted an increase in the arginine content of the chicken liver. The results of studies can be used to improve microelement preparations.


Lebedev S.,Orenburg State University | Yausheva E.,State Educational Institution All Russian Research Institute of Beef Cattle Breeding | Galaktionova L.,Orenburg State University | Sizova E.,Orenburg State University
Environmental Science and Pollution Research | Year: 2016

The influence of molybdenum oxide nanoparticles (MoO3) on the growth and survival of Eisenia fetida was established. The activity of antioxidant enzymes and changes in concentration of molybdenum in the body of E. fetida were determined. The degree of bacterial bioluminescence inhibition in extracts of substrates and worm was studied using luminescent strain Escherichia coli K12 TG1. The enzymatic activity of substrates before and after exposure with nanoparticles and worms was assessed. Nanoparticles have concentrations of 10, 40, and 500 mg/kg of dry matter, and substrata are made of artificial soil (substrate A) and microcrystalline cellulose (substrate B). Spherical nanoparticles MoO3, yellow in color, with size 92 ± 0.3 nm, Z-potential 42 ± 0.52 mV, molybdenum content 99.8 mass/%, and specific area 12 m2/g were used in the study. A significant decrease by 23.3 % in weight was registered (for MoO3 NPs at 500 mg/kg) on substrate A (p ≤ 0.05). On substrate B, the maximum decrease in weight by 20.5, 33.3, and 16.9 % (p ≤ 0.05) was registered at a dose of 10, 40, and 500 mg/kg, respectively; mortality was from 6.6 to 73 %. After the assessment of bacterial bioluminescence inhibition in substrates A and B (extracts) and before worms were put, the toxicity of substrates was established at doses of 40 and 500 mg/kg, expressed in inhibitory concentration (IC) 30 and IC 50 values. Comparatively, on days 7 and 14, after exposure in the presence of E. fetida, no inhibition of bioluminescence was registered in extracts of substrates A and B, indicating the reduction in toxicity of substrates. The initial content of molybdenum in E. fetida was 0.9 ± 0.018 mg/kg of dry matter. The degree of molybdenum accumulation in worm tissue was dependent on the dose and substrate quality. In particular, 2–7 mg/kg of molybdenum accumulated from substrate A, while up to 15 kg/kg of molybdenum accumulated from substrate B (day 7). Molybdenum concentration decreased by 64.8 and 57.4 % at doses 40 and 500 mg/kg, respectively, on day 14. The reaction of antioxidant enzymes was shown in an insignificant increase of glutathione reductase (GSR) and catalase (CAT) at concentrations of 10 and 40 mg/kg in substrate A, followed by the subsequent reduction of their activity at the dose of 500 mg/kg MoO3. The activity of GSR in substrate B against the presence of MoO3 nanoparticles decreased, with significant difference of 33.5 % (p ≤ 0.05) at the dose of 500 mg/kg compared with untreated soil. In experiments with substrate A, an increase of catalase activity was registered for the control sample. The presence of MoO3 nanoparticles at the concentration of 10 mg/kg in the environment promoted enzymatic activity on days 7 and 14, respectively. A further increase of nanoparticle concentration resulted in the decrease of catalase activity with a minimum value at the concentration of MoO3 of 500 mg/kg. In the experiment with substrate B at the concentration of MoO3 nanoparticles of 40 mg/kg, enzymatic activity increases on day 7 of exposure. However, the stimulating effect of nanoparticles stops by day 14 of the experiment and further catalase activity is dose dependent with the smallest value in the experiment with MoO3 having the concentration of 500 mg/kg. © 2016, Springer-Verlag Berlin Heidelberg.


PubMed | Orenburg State University and State Educational Institution All Russian Research Institute of Beef Cattle Breeding
Type: Journal Article | Journal: Environmental science and pollution research international | Year: 2016

The influence of molybdenum oxide nanoparticles (MoO3) on the growth and survival of Eisenia fetida was established. The activity of antioxidant enzymes and changes in concentration of molybdenum in the body of E. fetida were determined. The degree of bacterial bioluminescence inhibition in extracts of substrates and worm was studied using luminescent strain Escherichia coli K12 TG1. The enzymatic activity of substrates before and after exposure with nanoparticles and worms was assessed. Nanoparticles have concentrations of 10, 40, and 500mg/kg of dry matter, and substrata are made of artificial soil (substrate A) and microcrystalline cellulose (substrate B). Spherical nanoparticles MoO3, yellow in color, with size 920.3nm, Z-potential 420.52mV, molybdenum content 99.8 mass/%, and specific area 12m(2)/g were used in the study. A significant decrease by 23.3% in weight was registered (for MoO3 NPs at 500mg/kg) on substrate A (p0.05). On substrate B, the maximum decrease in weight by 20.5, 33.3, and 16.9% (p0.05) was registered at a dose of 10, 40, and 500mg/kg, respectively; mortality was from 6.6 to 73%. After the assessment of bacterial bioluminescence inhibition in substrates A and B (extracts) and before worms were put, the toxicity of substrates was established at doses of 40 and 500mg/kg, expressed in inhibitory concentration (IC) 30 and IC 50 values. Comparatively, on days 7 and 14, after exposure in the presence of E. fetida, no inhibition of bioluminescence was registered in extracts of substrates A and B, indicating the reduction in toxicity of substrates. The initial content of molybdenum in E. fetida was 0.90.018mg/kg of dry matter. The degree of molybdenum accumulation in worm tissue was dependent on the dose and substrate quality. In particular, 2-7mg/kg of molybdenum accumulated from substrate A, while up to 15kg/kg of molybdenum accumulated from substrate B (day 7). Molybdenum concentration decreased by 64.8 and 57.4% at doses 40 and 500mg/kg, respectively, on day 14. The reaction of antioxidant enzymes was shown in an insignificant increase of glutathione reductase (GSR) and catalase (CAT) at concentrations of 10 and 40mg/kg in substrate A, followed by the subsequent reduction of their activity at the dose of 500mg/kg MoO3. The activity of GSR in substrate B against the presence of MoO3 nanoparticles decreased, with significant difference of 33.5% (p0.05) at the dose of 500mg/kg compared with untreated soil. In experiments with substrate A, an increase of catalase activity was registered for the control sample. The presence of MoO3 nanoparticles at the concentration of 10mg/kg in the environment promoted enzymatic activity on days 7 and 14, respectively. A further increase of nanoparticle concentration resulted in the decrease of catalase activity with a minimum value at the concentration of MoO3 of 500mg/kg. In the experiment with substrate B at the concentration of MoO3 nanoparticles of 40mg/kg, enzymatic activity increases on day 7 of exposure. However, the stimulating effect of nanoparticles stops by day 14 of the experiment and further catalase activity is dose dependent with the smallest value in the experiment with MoO3 having the concentration of 500mg/kg.

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