State Committee of Forensic Examinations of the Republic of Belarus

Minsk, Belarus

State Committee of Forensic Examinations of the Republic of Belarus

Minsk, Belarus
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Borovko S.R.,State Committee of Forensic Examinations of the Republic of Belarus | Tillmar A.O.,National Board of Forensic Medicine | Tillmar A.O.,Linköping University | Kuzub N.N.,State Committee of Forensic Examinations of the Republic of Belarus | Rebala K.,Medical University of Gdańsk
Forensic Science International: Genetics Supplement Series | Year: 2015

Use of X-STR markers extends and gives additional possibilities to a forensic DNA expert for kinship analysis. FamLinkX is software for the calculation of likelihood ratios (LRs) for kinship cases based on X-STR DNA profiles that takes linkage and linkage disequilibrium as well as mutations of X-STR markers into account. Belarusian haplotype frequencies database of 360 unrelated Belarusian males has been uploaded to FamLinkX. LR values for 6 deficient paternity cases and 2 forensic cases involving two putative full siblings, females, were calculated with different values of lambda (λ = 1, 10, 100) in order to account for uncertainties in the haplotype frequency estimation. To demonstrate the ethnic sensitivity of X-STR markers and importance of the choice of the appropriate X-STR population database for LR calculation we calculated LR values for the same kinship cases using Belarusian and Swedish X-STR databases. LR values for the same kinship case differed from each other up to 6000 times depending on the chosen database. X-STRs genotyping lets us to determine relatedness successfully in all cases whereas the results of autosomal STR profiling and mitochondrial sequencing (for the cases where only the samples of putative full-siblings were available) were not sufficient to resolve family relationships issues. LR values were considered to be high enough in all cases. © 2015


Veremeichyk V.M.,State Committee of Forensic Examinations of the Republic of Belarus | Borovko S.R.,State Committee of Forensic Examinations of the Republic of Belarus | Kuzub N.N.,State Committee of Forensic Examinations of the Republic of Belarus
Forensic Science International: Genetics Supplement Series | Year: 2015

Autosomal loci with short tandem repeats (STR) are widely used for the human identification. Knowledge about the locus-specific mutation rates of STRs improves forensic probability calculations for the correct interpretation of diversity data and appropriate evaluation of genetic evidence in parentage and forensic analyses. Mutations of 17 STRs contained in two conventional kits of the AmpFlSTR® Identifiler® and PowerPlex®16 systems were studied in 11,744 paternity cases carried out during 2005–2015 in Belarus. In total, 283 mutations were identified at 15 from the 17 analyzed loci. The highest mutation rate was observed at FGA (0.00159), and the lowest at Penta E (0.00035). No mutation was observed at the TH01 and TPOX loci. © 2015 Elsevier Ireland Ltd


Borovko S.,State Committee of Forensic Examinations of the Republic of Belarus | Shyla A.,State Committee of Forensic Examinations of the Republic of Belarus | Korban V.,State Committee of Forensic Examinations of the Republic of Belarus | Borovko A.,State Committee of Forensic Examinations of the Republic of Belarus
Forensic Science International: Genetics | Year: 2015

Study of gender markers is a part of routine forensic genetic examination of crime scene and reference samples, paternity testing and personal identification. Amelogenin locus as a gender marker is included in majority of forensic STR kits of different manufacturers. In current study we report 11 cases of amelogenin abnormalities identified in males of Belarusian origin: 9 cases of AMELY dropout and 2 cases of AMELX dropout. Cases were obtained from forensic casework (n = 9) and paternity testing (n = 2) groups. In 4 out of 9 AMELY-negative cases deletion of AMELY was associated with the loss of DYS458 marker. In addition, we identified 3 males with SRY-positive XX male syndrome. Deletion of the long arm of the Y-chromosome was detected in two XX males. Loss of the major part of the Y-chromosome was identified in the third XX male. The presence of two X-chromosomes in XX males was confirmed with the use of Mentype® Argus X-8 PCR Amplification Kit. AMELY null allele observed in 2 out of 9 cases with AMELY dropout can be caused by mutation in the primer-binding site of AMELY allele. Primer-binding site mutations of AMELX can result in AMELX dropout identified in 2 cases with amplification failure of AMELX. Our study represents the first report and molecular genetic investigation of amelogenin abnormalities in the Belarusian population. © 2014 Elsevier Ireland Ltd.


PubMed | State Committee of Forensic Examinations of the Republic of Belarus
Type: | Journal: Forensic science international. Genetics | Year: 2015

Study of gender markers is a part of routine forensic genetic examination of crime scene and reference samples, paternity testing and personal identification. Amelogenin locus as a gender marker is included in majority of forensic STR kits of different manufacturers. In current study we report 11 cases of amelogenin abnormalities identified in males of Belarusian origin: 9 cases of AMELY dropout and 2 cases of AMELX dropout. Cases were obtained from forensic casework (n=9) and paternity testing (n=2) groups. In 4 out of 9 AMELY-negative cases deletion of AMELY was associated with the loss of DYS458 marker. In addition, we identified 3 males with SRY-positive XX male syndrome. Deletion of the long arm of the Y-chromosome was detected in two XX males. Loss of the major part of the Y-chromosome was identified in the third XX male. The presence of two X-chromosomes in XX males was confirmed with the use of Mentype() Argus X-8 PCR Amplification Kit. AMELY null allele observed in 2 out of 9 cases with AMELY dropout can be caused by mutation in the primer-binding site of AMELY allele. Primer-binding site mutations of AMELX can result in AMELX dropout identified in 2 cases with amplification failure of AMELX. Our study represents the first report and molecular genetic investigation of amelogenin abnormalities in the Belarusian population.

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