Kurumbapet, India
Kurumbapet, India

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Sekar D.,Pondicherry Center for Biological science | Saravanan S.,Pondicherry Center for Biological science | Karikalan K.,Vellore Institute of Technology | Thirugnanasambantham K.,Pondicherry Center for Biological science | And 3 more authors.
Current Pharmaceutical Biotechnology | Year: 2015

The significant role of microRNAs (miRNAs) in stem cell development has been expansively discussed in recent publications and they are noticed to be involved in post transcriptional regulation of gene expression by its affinity towards the 3′UTR of target mRNA. Since the expression of miR-21 was high in cells that were derived from MSCs, recent evidence indicates that miR-21 plays a vital role in mesenchymal stem cells (MSCs) differentiation. Despite the fact that miR-21 can be considered as a powerful biomarker for MSCs differentiation, the number of studies related to the above scenario is very limited. This review highlights the recent publications related to the importance of miR-21 specifically in differentiation of MSCs. © 2015 Bentham Science Publishers.


Sekar D.,Pondicherry Center for Biological science | Thirugnanasambantham K.,Pondicherry Center for Biological science | Thirugnanasambantham K.,State Bio control Laboratory | Hairul Islam V.I.,Pondicherry Center for Biological science | And 2 more authors.
Cell Proliferation | Year: 2014

Use of sequencing approaches is an important aspect in the field of cancer genomics, where next-generation sequencing has already been utilized for targeting oncogenes or tumour-suppressor genes, that can be sequenced in a short time period. Alterations such as point mutations, insertions/deletions, copy number alterations, chromosomal rearrangements and epigenetic changes are encountered in cancer cell genomes, and application of various NGS technologies in cancer research will encounter such modifications. Rapid advancement in technology has led to exponential growth in the field of genomic analysis. The $1000 Genome Project (in which the goal is to sequence an entire human genome for $1000), and deep sequencing techniques (which have greater accuracy and provide a more complete analysis of the genome), are examples of rapid advancements in the field of cancer genomics. In this mini review, we explore sequencing techniques, correlating their importance in cancer therapy and treatment. © 2014 John Wiley & Sons Ltd.


Thirugnanasambantham K.,State Bio control Laboratory | Thirugnanasambantham K.,Pondicherry Center for Biological science | Durairaj S.,Pondicherry Center for Biological science | Saravanan S.,Pondicherry Center for Biological science | And 3 more authors.
Plant Molecular Biology Reporter | Year: 2015

Plants are nonmotile and are easily affected by both biotic and abiotic stresses. Plants have evolved themselves at both cellular and molecular level to fight against stress. Transcription factors are important among the stress-responsive genes, and their protein products are known to regulate the expression of other stress-responsive genes via binding to the regulatory elements. Among the plant transcription factors, ethylene response factor (ERF) is one of the largest subfamilies of Apetala2 (AP2)/ERF transcription factor family and is characterized with single AP2 domain. ERFs are a double-edged sword; though most of the ERFs are activators of stress-responsive genes, certain ERF could act as repressor, and this phenomenon of ERF has been well discussed in this review. Further, the expression of ERFs may be ethylene dependent or independent and is regulated by feedback mechanism. Apart from above regulation mechanism, expressions of ERFs are post-transcriptionally regulated by microRNAs (miRNAs), and miRNA expressions are in turn regulated by ERFs. The present review highlights the importance of ERFs in plant stress management and complexity in regulation of ERF expression in response to various stresses. © 2014, Springer Science+Business Media New York.


Thirugnanasambantham K.,Pondicherry Center for Biological science | Thirugnanasambantham K.,State Bio control Laboratory | Saravanan S.,Pondicherry Center for Biological science | Karikalan K.,Vellore Institute of Technology | And 4 more authors.
Computational Biology and Chemistry | Year: 2015

Momordica charantia (bitter gourd, bitter melon) is a monoecious Cucurbitaceae with anti-oxidant, anti-microbial, anti-viral and anti-diabetic potential. Molecular studies on this economically valuable plant are very essential to understand its phylogeny and evolution. MicroRNAs (miRNAs) are conserved, small, non-coding RNA with ability to regulate gene expression by bind the 3′ UTR region of target mRNA and are evolved at different rates in different plant species. In this study we have utilized homology based computational approach and identified 27 mature miRNAs for the first time from this bio-medically important plant. The phylogenetic tree developed from binary data derived from the data on presence/absence of the identified miRNAs were noticed to be uncertain and biased. Most of the identified miRNAs were highly conserved among the plant species and sequence based phylogeny analysis of miRNAs resolved the above difficulties in phylogeny approach using miRNA. Predicted gene targets of the identified miRNAs revealed their importance in regulation of plant developmental process. Reported miRNAs held sequence conservation in mature miRNAs and the detailed phylogeny analysis of pre-miRNA sequences revealed genus specific segregation of clusters. © 2015 Elsevier Ltd. All rights reserved.


PubMed | State Bio control Laboratory, Narayana Medical College and Hospital, Pondicherry Center for Biological science, Cryovault Biotech India Pvt. Ltd and Pondicherry University
Type: Journal Article | Journal: Clinics and research in hepatology and gastroenterology | Year: 2016

Despite promising developments of treatment, the mortality due to gastric cancer remains high and the mechanisms of gastric cancer initiation and the development also remains elusive. It has been reported that patients with positive serologic tests for H.pylori have a higher risk of the development of gastric cancer. microRNAs (miRNAs) are short non-coding RNA molecules consisting of 21-25 nucleotides (nt) in length. The miRNAs silence their cognate target genes by inhibiting mRNA translation or degrading the mRNA molecules by binding to their 3-untranslated (UTR) regions and plays a very important role in cancer biology. Recent evidences indicate that miR-21 is overexpressed in tumour tissue, including gastric cancer and plays a vital role in tumour cell proliferation, apoptosis, invasion and angiogenesis. Elevated levels of miR-21 is associated with downregulation of tumour suppressor genes, such as programmed cell death 4 (PDCD4), tissue inhibitor of metalloproteinase 3, phosphatase and tensin homolog (PTEN), tropomyosin 1, ras homolog gene family member B, and maspin. Silencing of miR-21 through the use of a miR-21 inhibitor affected cancer cell viability, induced cell cycle arrest and increased chemosensitivity to anticancer agents indicating that miR-21 functions as an oncogene. Although an increased expression level of miR-21 has been observed in gastric cancer, studies related to the role of miR-21 in gastric cancer progression is very limited. The main thrust of this mini review is to explain the potency of miR-21 as a prognostic and/or diagnostic biomarker and as a new target for clinical therapeutic for interventions of gastric cancer progression.


Thirugnanasambantham K.,UPASI Tea Research Foundation | Thirugnanasambantham K.,State Bio Control Laboratory | Muralidaran S.,UPASI Tea Research Foundation | Mandal A.K.A.,UPASI Tea Research Foundation | Mandal A.K.A.,Vellore Institute of Technology
Applied Biochemistry and Biotechnology | Year: 2014

Tea [Camellia sinensis (L.) O. Kuntze] is one of the most popular non-alcoholic beverages rich in phenolic compounds, which includes epigallocatechin gallate (EGCG), epigallocatechin (EGC), epicatechin gallate (ECG), epicatechin (EC) and catechin (C). Anthocyanidin reductase (ANR) is responsible for catechin biosynthesis in plants, and analysis of its protein sequences and structures will be valuable for further research in the field. We have screened our dormant bud-specific complementary DNA (cDNA) library and reported 1,322-bp cDNA encoding CsANR. Analysis of the sequence revealed the presence of 1,011-bp open reading frame with coding capacity for a polypeptide of 337 amino acids, flanked by 1,123- and 196-bp 5′ and 3′ untranslated regions, respectively. Theoretical molecular weight (MW) and isoelectric point (pI) of the deduced ANR protein were predicted (using ProtParam) to be 36.4 kDa and 6.54. For the first time, we have reported 3D model of ANR from C. sinensis. Quality of the predicted model was analysed with PROCHECK analysis. Molecular docking of modelled ANR revealed similar binding pockets for both substrates and products. Expression analyses of CsANR and accumulation pattern of catechins were observed to be varied with developmental age of tissue and seasonal condition. Variation in accumulation pattern of catechins and its fractions was found to be correlated with expression pattern of ANR. © 2014 Springer Science+Business Media.

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