Lachmann K.,Fraunhofer Institute for Surface Engineering and Thin Films |
Dohse A.,Fraunhofer Institute for Surface Engineering and Thin Films |
Thomas M.,Fraunhofer Institute for Surface Engineering and Thin Films |
Pohl S.,Helmholtz Center for Infection Research |
And 4 more authors.
HAKONE 2010 - 12th International Symposium on High Pressure Low Temperature Plasma Chemistry | Year: 2010
In modern medicine human mesenchymal stem cells are gaining increasing importance. Using helium-based gas mixtures (He + N2 + H2) as well as helium enriched with suitable film-forming agents the inner surface of polymer bags can be modified with a plasma process developed at Fraunhofer IST. The effect of plasma treatment is investigated by FTIR-ATR spectroscopy as well as surface tension determination based on contact angle measurements. Plasma treatment in nominally pure helium increases the surface tension of the polymer foil due to the presence of oxygen traces in the gas and oxygen diffusing through the gas-permeable foil, resp., reacting with surface radical centres formed in the discharge. For the same reason the area density of primary amino groups on the surface obtained by treatment in mixtures with nitrogen is comparably small. With regard to their applicability in cell cultivation three different coatings (bases on APTMS, DACH and TMOS) were tested. It is shown that primary amino groups play an important role in cell cultivation of adherent growing bone marrow mesenchymal stem cells.
He F.,Helmholtz Center for Infection Research |
He F.,University of Luxembourg |
Chen H.,Helmholtz Center for Infection Research |
Probst-Kepper M.,Stadtisches Klinikum Braunschweig GmbH |
And 7 more authors.
Molecular Systems Biology | Year: 2012
Human FOXP3 + CD25 + CD4 + regulatory T cells (Tregs) are essential to the maintenance of immune homeostasis. Several genes are known to be important for murine Tregs, but for human Tregs the genes and underlying molecular networks controlling the suppressor function still largely remain unclear. Here, we describe a strategy to identify the key genes directly from an undirected correlation network which we reconstruct from a very high time-resolution (HTR) transcriptome during the activation of human Tregs/CD4 + T-effector cells. We show that a predicted top-ranked new key gene PLAU (the plasminogen activator urokinase) is important for the suppressor function of both human and murine Tregs. Further analysis unveils that PLAU is particularly important for memory Tregs and that PLAU mediates Treg suppressor function via STAT5 and ERK signaling pathways. Our study demonstrates the potential for identifying novel key genes for complex dynamic biological processes using a network strategy based on HTR data, and reveals a critical role for PLAU in Treg suppressor function. © 2012 EMBO and Macmillan Publishers Limited All rights reserved.