News Article | November 23, 2016
TAMPA, Fla., Nov. 23, 2016 /PRNewswire-USNewswire/ -- The holiday season is upon us, which makes it prime time for toy buying. In fact, more than 65 percent of the toys and games sold in the United States each year are purchased between the Friday after Thanksgiving and Christmas. While...
News Article | October 31, 2016
TAMPA, Fla., Oct. 31, 2016 /PRNewswire-USNewswire/ -- Superheroes, pint-sized princesses and Pokémon characters invaded the hallways of St. Joseph's Children's Hospital in Tampa today. Some came on foot, others arrived in little red wagons and wheelchairs, and all were in search of...
Mikhail F.M.,University of Alabama at Birmingham |
Burnside R.D.,Laboratory Corporation of America |
Rush B.,Laboratory Corporation of America |
Ibrahim J.,St Josephs Childrens Hospital |
And 5 more authors.
Genetics in Medicine | Year: 2014
Purpose:The five segmental duplications (LCR22-D to-H) at the distal region of chromosome 22 band q11.2 in the region immediately distal to the DiGeorge/velocardiofacial syndrome deleted region have been implicated in the recurrent distal 22q11.2 microdeletions. To date, the distal 22q11.2 microdeletions have been grouped together as a single clinical entity despite the fact that these deletions are variable in size and position depending on the mediating LCR22s.Methods:Here, we report 13 new unrelated patients with variable size deletions in the distal 22q11.2 region as shown by cytogenomic array analyses. We compare our patients' clinical features with those of previously reported cases to better dissect the phenotypic correlations based on the deletion size and position.Results:Six patients had the 1.1-Mb deletion flanked by LCR22-D and-E, and presented clinically with a phenotype consistent with previously reported cases with distal 22q11.2 microdeletions. Three patients had the 1.8-Mb deletion flanked by LCR22-D and-F, and presented with a similar phenotype. Four patients had the 700-kb deletion flanked by LCR22-E and-F, and presented with a milder phenotype that lacked growth restriction and cardiovascular defects.Conclusion:We suggest that the recurrent distal 22q11.2 microdeletions do not represent a single clinical entity, and propose categorizing these deletions into three types according to their genomic position. All three deletion types are thought to be pathogenic and are most often de novo. They all share some presenting features but also have their unique features and risks. © American College of Medical Genetics and Genomics.
Chawla M.,Immunology Group |
Parikh P.,Immunology Group |
Saxena A.,Immunology Group |
Munshi M.,Immunology Group |
And 10 more authors.
Molecular Microbiology | Year: 2012
Host-generated oxidative stress is considered one of the main mechanisms constraining Mycobacterium tuberculosis (Mtb) growth. The redox-sensing mechanisms in Mtb are not completely understood. Here we show that WhiB4 responds to oxygen (O2) and nitric oxide (NO) via its 4Fe-4S cluster and controls the oxidative stress response in Mtb. The WhiB4 mutant (MtbΔwhiB4) displayed an altered redox balance and a reduced membrane potential. Microarray analysis demonstrated that MtbΔwhiB4 overexpresses the antioxidant systems including alkyl hydroperoxidase (ahpC-ahpD) and rubredoxins (rubA-rubB). DNA binding assays showed that WhiB4 [4Fe-4S] cluster is dispensable for DNA binding. However, oxidation of the apo-WhiB4 Cys thiols induced disulphide-linked oligomerization, DNA binding and transcriptional repression, whereas reduction reversed the effect. Furthermore, WhiB4 binds DNA with a preference for GC-rich sequences. Expression analysis showed that oxidative stress repressed whiB4 and induced antioxidants in Mtb, while their hyper-induction was observed in MtbΔwhiB4. MtbΔwhiB4 showed increased resistance to oxidative stress in vitro and enhanced survival inside the macrophages. Lastly, MtbΔwhiB4 displayed hypervirulence in the lungs of guinea pigs, but showed a defect in dissemination to their spleen. These findings suggest that WhiB4 systematically calibrates the activation of oxidative stress response in Mtb to maintain redox balance, and to modulate virulence. © 2012 Blackwell Publishing Ltd.
Kardashian G.,New York University |
Strongwater A.M.,St Josephs Childrens Hospital
Journal of Pediatric Orthopaedics Part B | Year: 2010
This report is a case of slipped capital femoral epiphysis in a nonambulatory patient with spastic quadriplegic type of cerebral palsy. Such a case is unusual as no weight-bearing forces were acting on the hip; however, spasticity may have played a role. To the best of our knowledge no earlier cases have been reported in the literature. © 2010 Wolters Kluwer Health | Lippincott Williams & Wilkins.
Ringewald J.M.,St Josephs Childrens Hospital
Current Opinion in Cardiology | Year: 2016
Purpose of review: Transcatheter pulmonary valve replacement has only been both approved and widely available for most congenital heart disease centers for a few years; its use and familiarity for interventionalists have greatly expanded our knowledge of its applicability to a multitude of clinical situations. Expanded worldwide use and longer time from implant have both served to better understand procedural limits and uncommon late adverse events. Recent findings: Although currently approved for implantation in the USA only in dysfunctional and circumferential right ventricle to pulmonary artery conduits, with expanded experience operators have been able to adapt the delivery of this valve in a large number of additional clinical scenarios. Rare technical limitations of the procedure, most importantly coronary compression, are now being better defined. Although not frequent, a significant number of infective endocarditis episodes have been reported, but more recently several studies have deepened our understanding of this late adverse event for the most commonly implanted transcatheter pulmonary valve prosthesis. Summary: Expanded and widened use has extended our understanding of who may benefit from transcatheter pulmonary valve implantation (TPVI), the current limits of TPVI, and uncommon but important late issues following TPVI. Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved.
Ghersin Z.J.,St Josephs Childrens Hospital |
Kuo D.J.,Rady Childrens Hospital San Diego
Journal of Pediatric Hematology/Oncology | Year: 2016
Melanotic neuroectodermal tumor of infancy is a rare benign tumor of neural crest origin. The tumor generally presents in the jawbones; however, it occasionally occurs in extracranial sites. Although 95% of these tumors present within the first year of life and 15% in extracranial locations, we report an unusual case of a 15-month-old male with melanotic neuroectodermal tumor of infancy of the epididymis. The patient underwent orchiectomy without adjuvant chemotherapy or radiation. Twenty months later, there was no sign of recurrence. In addition, we discuss the role of chemotherapy and radiation and the potential importance of molecular genetics in establishing guidelines for management. © 2016 Wolters Kluwer Health, Inc. All rights reserved.
Pourtaheri N.,Seton Hall University |
Pourtaheri N.,St Josephs Childrens Hospital |
Strongwater A.M.,St Josephs Childrens Hospital
Orthopedics | Year: 2012
Posttraumatic clavicle nonunion is rare, particularly in children. Four cases of clavicle fracture nonunion in patients aged 10 years and younger have been reported. A variety of techniques have been used to treat pediatric clavicle nonunions. A 10-year-old boy presented to our institution after a fall, sustaining a right closed midshaft clavicle fracture. No other injuries occurred, and neurovascular examination of the right upper extremity was normal. The fracture was initially treated with a sling for >4 months, and the fracture progressed to a hypertrophic nonunion. Serial radiographs failed to demonstrate progression to union. The patient continued to have pain with activity 4 months after his injury. Clavicle pseudarthrosis was considered; however, radiographs did not have the characteristic appearance of this condition. This fracture nonunion was treated with internal fixation and united with no complication. The patient was back to full activities of daily living 6 months postoperatively. He reported no tenderness at the fracture site or along the hardware. The treating surgeon (A.M.S.) prefers to remove hardware in young children, but the family declined removal. Pediatric posttraumatic nonunion of the clavicle is rare but can be safely treated with plate fixation, with excellent results.
De A.,St Josephs Childrens Hospital |
Myridakis D.,St Josephs Childrens Hospital |
Kerrigan M.,St Josephs Childrens Hospital |
Kiblawi F.,St Josephs Childrens Hospital
Texas Heart Institute Journal | Year: 2011
Varicella (chickenpox), a common childhood infection caused by the varicella-zoster virus, is self-limiting and usually benign. Although atypical manifestations of the virus are occasionally seen, it rarely presents with cardiovascular sequelae. Cardiovascular complications of varicella can include pericarditis, myocarditis, or endocarditis. Herein, we report the case of a 17-year-old boy who had varicella infection and severe chest pain. Examination revealed atypical electrocardiographic findings of pericarditis and remarkably elevated cardiac biomarker levels: peak cardiac troponin I, 37.2 ng/mL; total creatine kinase, 1,209 U/L; and creatine kinase-MB fraction, 133.6 ng/mL. After results of coronary angiography reliably excluded ischemia and myocardial infarction, the diagnosis was varicella myopericarditis. The patient was placed on a medical regimen during and after 5 days of hospitalization. In 2 weeks, he was asymptomatic, and at 6 months, he was doing well and had normal electrocardiographic and echocardiographic results. To our knowledge, cardiac enzyme elevations to these levels have not been reported in cases of cardiovascular sequelae of varicella. We discuss the diagnostic challenges of this atypical case and suggest that clinicians be aware that varicella disease is most often, but not always, benign. © 2011 by the Texas Heart ® Institute, Houston.
St Josephs Childrens Hospital | Date: 2012-02-10
Several bacterial species were isolated from marine segment obtained from seabed sediment at depths exceeding 1700 feet. At least four of the bacteria produced a compound that showed antibacterial activity against one or more multiple-drug-resistant (MDR) bacteria isolated from hospitals and clinics. One isolate, SJCH-12, exhibited a broad range of activity against MDR strains tested, including methicillin resistant Staphylococcus aureus (MRSA).