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Bangalore, India

Vijaya Bhaskar S.,St Johns Pharmacy College | Seshagiri Rao J.V.L.N.,Andhra University | Sree V. Vijaya,St Johns Pharmacy College
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2013

A rapid, simple, reliable, accurate, and sensitive high performance liquid chromatography method for the determination of Tolterodine in raw materials and tablet dosage forms was developed and validated. Tolterodine was chromatogramed on Kromosil Symmetry C18 (150 × 4.6 mm; 5 μm) using a mobile phase consisting of phosphate buffer (pH 3.0) and acetonitrile in the ratio of 45:55 v/v. The flow rate was maintained at 1ml/min and eluents were detected with the retention time of 2.25 min. at 284 nm by using UV detector absorbance. The proposed method was validated by determining accuracy, precision, and linearity was observed in the range of 20 - 100μg/mL of the drug. The mean recovery of the drug was indicating high level of accuracy of the method. Due to its simplicity, accuracy and high precision of the proposed HPLC method was found to be appropriate for the estimation of Tolterodine in bulk and pharmaceutical dosage forms. Source


Suresh Kumar G.V.,St Johns Pharmacy College | Rajendraprasad Y.,Andhra University | Chandrashekar S.M.,St Johns Pharmacy College | Kistayya C.,Andhra University
European Journal of Medicinal Chemistry | Year: 2010

In the present study a series of 2-substituted-5-[isopropylthiazole] clubbed 1,2,4-triazole and 1,3,4-oxadiazole derivatives have been synthesized and characterized by IR, 1H NMR, 13C NMR and mass spectral analysis. Synthesized compounds were evaluated for their preliminary cytotoxicity, antimicrobial and antitubercular activity against Mycobacterium tuberculosis H37Rv strain by broth dilution assay method. Antimycobacterial activity tested against M. tuberculosis indicated that compounds 4b and 6g exhibited twofold enhanced potency than parent compound 1 and the results indicate that some of them exhibited promising activities and they deserve more consideration as potential antitubercular agents. Compound 3c, 4b and 6c exhibited good or moderate antibacterial inhibition and compounds 3h and 7c showed excellent antifungal activity. © 2010 Elsevier Masson SAS. All rights reserved. Source


Vijaya Bhaskar S.,St Johns Pharmacy College | Seshagiri Rao J.V.L.N.,St Johns Pharmacy College | Seetha Ramaiah P.,Andhra University
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2013

A simple, rapid, accurate, reliable, and sensitive high performance liquid chromatography method for the determination of Trapidil in raw materials and tablet dosage forms was developed and validated. Trapidil was chromatogramed on Hypersil Symmetry C18, (100 X 4.6 mm; 5 μm) using a mobile phase consisting of phosphate buffer (pH 3.0) and acetonitrile in the ratio of 45:55 v/v. The flow rate was maintained at 0.9 ml/min and eluents were detected with the retention time of 2.22 min. at 221 nm by using UV detector absorbance. The proposed method was validated by determining accuracy, precision, and linearity was observed in the range of 10 - 50 μg/mL of the drug. The mean recovery of the drug was indicating high level of accuracy of the method. Due to its simplicity, accuracy and high precision of the proposed HPLC method was found to be appropriate for the estimation of Trapidil in bulk and pharmaceutical dosage forms. Source


Kathiriya A.,St Johns Pharmacy College | Das K.,St Johns Pharmacy College | Kumar E.P.,St Johns Pharmacy College | Mathai K.B.,St Johns Pharmacy College
Iranian Journal of Cancer Prevention | Year: 2010

Background: Present investigations were carried out to evaluate an ethanolic extract of Oxalis corniculata Linn. For its anticancer and antioxidant activity in Ehrlich acsites carcinoma (EAC) - induced in swiss albino mice. Methods: Cancer was induced in mice by aspiration of peritoneal fluid of EAC inoculated mice and diluted to get 1x106 cells/ml in phosphate buffer. Cancerous animals were divided into four groups having six mice in each in which Group III and IV cancerous animals were treated with the ethanolic extract of Oxalis corniculata Linn. One hundred and 400 mg/kg b.w. respectively, whereas Group V cancerous animals received standard drug cyclophosphamide 25 mg/kg b.w. The test and standard drugs were administered orally 24 hrs after the inoculation of EAC for 9 days. The several changes of antitumor potential of EEOC were accessed. Biochemical parameters such as total protein, albumin, Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP), lipid peroxidation and enzymatic antioxidants were determined. Results: The present study revealed that EEOC showed significant antitumor and antioxidant activities in EAC bearing mice. The dose dependent reduction in body weight, tumor volume, packed cell volume, tumor cell counts and increase in median survival time (MST) and percentage increase in life span in EEOC treated animals were observed. There was a significant increase in RBC count; Hb content in EEOC treated animals and reduction in total WBC count. There was a significant increase in total protein and albumin content, but decrease in AST, ALT and ALP contents in EEOC treated animals. A significant decrease in liver MDA levels and increase in catalase and reduced glutathione levels were observed in EEOC treated animals. Conclusion: Results conclude that the EEOC was effective in inhibiting the tumor growth in ascitic and solid tumor models. The biochemical and antioxidants studies were also supported its antitumor properties. Source

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