St Johns Pharmacy College

Bangalore, India

St Johns Pharmacy College

Bangalore, India
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Senthil Kumar K.K.,St Johns Pharmacy College
Pharmacologyonline | Year: 2010

The present study is an investigation of anti-epileptic activity of Xanthium Strumarium L. (Family-compositae) is a well known plant which is being used in Indian traditional medicines for treating epilepsy, inflammation and tuberculosis. The petroleum ether extract of Xanthium Strumarium L. (PEXS) was subjected to acute toxicity and then screened for anticonvulsant activity on Maximal Electroshock (MES) and Pentylenetetrazole (PTZ) induced seizures models in albino wistar rats. Acute toxicity of extract was non toxic up to the recommended dose 2000 mg/kg. p.o. Animals were treated with PEXS at doses of 250 and 500 mg/kg body weight. Study results showed, the mean duration of extensor phase of treated groups reduced significant level than compared to control group. In Pentylenetetrazol induced seizure model, onset of myoclonic spasm and clonic convulsion was delayed in the PEXS treated groups. PEXS showed anti-epileptic activity against MES and PTZ animal models. However, further studies still needed to be carried on exposure of the extract to humans.


Das K.,St Johns Pharmacy College | Kathiriya A.K.,St Johns Pharmacy College | Kumar E.P.,Karpagaram University | Benson M.K.,St Johns Pharmacy College | Einstein J.W.,St Johns Pharmacy College
Brazilian Journal of Pharmacognosy | Year: 2012

The present study was carried out to evaluate the hepatoprotective activity of aqueous and ethanolic leaves extracts of Oxalis corniculata L., Oxalidaceae, against thioacetamide-induced hepatotoxicity. Hepatotoxicity was induced in Wistar rats of either sex by subcutaneous injection of thioacetamide. An aqueous and ethanolic extract of aerial parts of O. corniculata (200 and 400 mg/kg/day) were evaluated. Oral administration of O. corniculata aqueous and ethanolic leaves extract at 400 mg/kg resulted in a significant reduction in SGOT (146.42±2.54 and 136.75±1.37 IU/L respectively), SGPT (81.96±3.15 and 72.05±2.33 IU/L respectively), GGTP (16.6±0.49 and 15.02±0.68 IU/L respectively), ALP (241.86±3.94 and 202.42±5.37 IU/L respectively) and total bilirubin (0.226±0.00 mg/dL 0.288±0.01 mg/dL respectively) content that were lesser than positive control, thioacetamide damaged rats. Histology of the liver sections of the animals treated with the extract also showed dose dependent reduction of necrosis. Hence the study concluded that O. corniculata has potential hepatoprotective activity.


Suresh Kumar G.V.,St Johns Pharmacy College | Rajendraprasad Y.,Andhra University
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2010

A simple, precise and accurate reversed-phase liquid chromatographic method was developed and validated for simultaneous estimation of Telmisartan and Amlodipine in tablet formulations. The chromatographic separation was achieved on (Waters symmetry C18 250mm x 4.6mm 5μm) analytical column with mobile phase consisting mixture of Potassium dihydrogen phosphate (0.02M, pH 3.0 adjusted with ortho-phosphoric acid) and acetonitrile in ratio (60:40 v/v) at flow rate of 1.5ml/min and detector wavelength 237 nm. The retention time of Amlodipine and telmisartan was found to be 3.5 and 8.1 minutes respectively. The validation of the proposed method was carried out for its specificity, linearity, accuracy, precision, limit of detection and quantification for both Telmisartan and Amlodipine. The developed method can be used for routine quality analysis of titled drugs in combination in tablet formulation.


Suresh Kumar G.V.,St Johns Pharmacy College | Rajendraprasad Y.,Andhra University | Chandrashekar S.M.,St Johns Pharmacy College | Kistayya C.,Andhra University
European Journal of Medicinal Chemistry | Year: 2010

In the present study a series of 2-substituted-5-[isopropylthiazole] clubbed 1,2,4-triazole and 1,3,4-oxadiazole derivatives have been synthesized and characterized by IR, 1H NMR, 13C NMR and mass spectral analysis. Synthesized compounds were evaluated for their preliminary cytotoxicity, antimicrobial and antitubercular activity against Mycobacterium tuberculosis H37Rv strain by broth dilution assay method. Antimycobacterial activity tested against M. tuberculosis indicated that compounds 4b and 6g exhibited twofold enhanced potency than parent compound 1 and the results indicate that some of them exhibited promising activities and they deserve more consideration as potential antitubercular agents. Compound 3c, 4b and 6c exhibited good or moderate antibacterial inhibition and compounds 3h and 7c showed excellent antifungal activity. © 2010 Elsevier Masson SAS. All rights reserved.


The present investigation was aimed to study an anticonvulsant activity of aqueous extract of Desmodium triflorum (L.) DC., Fabaceae, in mice. Animal models of epilepsy namely the pentylenetetrazole, and maximal electroshock induced convulsion were used to evaluate the anticonvulsant effects of the extracts. The biochemical estimation was done by measuring the lipid peroxidation and reduced glutathione. In the pentylenetetrazole induced convulsion, aqueous extract of D. triflorum 800 mg/kg significant delayed the onset of convulsion, reduced the duration of convulsion (p<0.05) and reduced mortality. The aqueous extract of D. triflorum 800 mg/kg dose reduced hind limb tonic extension phase of maximal electroshock induced convulsion induced convulsion in mice (p<0.05). The pretreated aqueous extract of D. triflorum showed significant inhibition of lipid peroxidation and increases the reduced glutathione level in mice brain tissue (p<0.001). The results revealed that D. triflorum possesses a significant dose dependent anticonvulsant activity.


Dabheliya J.,St Johns Pharmacy College | Khan S.A.,St Johns Pharmacy College | Joshipura M.,St Johns Pharmacy College | Vasoya M.,St Johns Pharmacy College | And 2 more authors.
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2010

Withania coagulans has been widely used in folklore medicines. The diuretic activity of the aqueous extract of fruits of Withania coagulans was studied by invivo Lipschitz test model with slight modifications using furosemide as a standard. The volumes of urine, urinary concentration of sodium, potassium and chloride ions were the parameters of the study. The results indicated significant (P < 0.001) increase in the urine volume by 79.12 % and 71.02 % in 500 mg/kg and 750 mg/kg doses respectively, when compared to control group. Urinary electrolyte excretions were increased with both the doses when compared to control. Both the doses showed significant excretion of electrolytes but on contrary; 500 mg/kg dose was found to be more significant as compared to 750 mg/kg dose. From the present study, the diuretic activity of the aqueous extract of Withania coagulans has been justified and further confirms its use as a diuretic agent.


Gowda G.,St Johns Pharmacy College | Bhosle V.,St Johns Pharmacy College | Einstein J.W.,St Johns Pharmacy College | Das K.,St Johns Pharmacy College | Benson Mathai K.,St Johns Pharmacy College
Brazilian Journal of Pharmacognosy | Year: 2012

The present investigation was aimed to study an anticonvulsant activity of ethanolic extract of Desmodium triflorum (L.) DC., Fabaceae, in mice. Animal models of epilepsy namely the pentylenetetrazole (PTZ), isoniazid or isonicotinic hydrazide (INH) and maximal electroshock induced convulsion (MES) were used to evaluate the anticonvulsant effects of the extracts. The biochemical estimation was done by measuring the lipid peroxidation and reduced glutathione (GSH). In the PTZ induced convulsion, ethanolic extract of D. triflorum (EEDT) 400 mg/kg significant delayed the onset of convulsion, reduced the duration of convulsion and reduced mortality. Similarly a dose of 800 mg/kg of EDDT significantly delayed the onset of convulsion, reduced the duration of convulsion and showed 33.33% protection in mice against INH induced convulsion. Further no mortality was found. Both the doses reduced hind limb tonic extension (HLTE) phase of MES induced convulsion in mice. The pretreated EEDT showed significant inhibition of lipid peroxidation and increases the reduced glutathione level in mice brain tissue. The results revealed that D. triflorum possesses a significant dose dependent anticonvulsant activity.


Vijaya Bhaskar S.,St Johns Pharmacy College | Seshagiri Rao J.V.L.N.,St Johns Pharmacy College | Seetha Ramaiah P.,Andhra University
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2013

A simple, rapid, accurate, reliable, and sensitive high performance liquid chromatography method for the determination of Trapidil in raw materials and tablet dosage forms was developed and validated. Trapidil was chromatogramed on Hypersil Symmetry C18, (100 X 4.6 mm; 5 μm) using a mobile phase consisting of phosphate buffer (pH 3.0) and acetonitrile in the ratio of 45:55 v/v. The flow rate was maintained at 0.9 ml/min and eluents were detected with the retention time of 2.22 min. at 221 nm by using UV detector absorbance. The proposed method was validated by determining accuracy, precision, and linearity was observed in the range of 10 - 50 μg/mL of the drug. The mean recovery of the drug was indicating high level of accuracy of the method. Due to its simplicity, accuracy and high precision of the proposed HPLC method was found to be appropriate for the estimation of Trapidil in bulk and pharmaceutical dosage forms.


Vijaya Bhaskar S.,St Johns Pharmacy College | Seshagiri Rao J.V.L.N.,Andhra University | Sree V. Vijaya,St Johns Pharmacy College
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2013

A rapid, simple, reliable, accurate, and sensitive high performance liquid chromatography method for the determination of Tolterodine in raw materials and tablet dosage forms was developed and validated. Tolterodine was chromatogramed on Kromosil Symmetry C18 (150 × 4.6 mm; 5 μm) using a mobile phase consisting of phosphate buffer (pH 3.0) and acetonitrile in the ratio of 45:55 v/v. The flow rate was maintained at 1ml/min and eluents were detected with the retention time of 2.25 min. at 284 nm by using UV detector absorbance. The proposed method was validated by determining accuracy, precision, and linearity was observed in the range of 20 - 100μg/mL of the drug. The mean recovery of the drug was indicating high level of accuracy of the method. Due to its simplicity, accuracy and high precision of the proposed HPLC method was found to be appropriate for the estimation of Tolterodine in bulk and pharmaceutical dosage forms.


Kathiriya A.,St Johns Pharmacy College | Das K.,St Johns Pharmacy College | Kumar E.P.,St Johns Pharmacy College | Mathai K.B.,St Johns Pharmacy College
Iranian Journal of Cancer Prevention | Year: 2010

Background: Present investigations were carried out to evaluate an ethanolic extract of Oxalis corniculata Linn. For its anticancer and antioxidant activity in Ehrlich acsites carcinoma (EAC) - induced in swiss albino mice. Methods: Cancer was induced in mice by aspiration of peritoneal fluid of EAC inoculated mice and diluted to get 1x106 cells/ml in phosphate buffer. Cancerous animals were divided into four groups having six mice in each in which Group III and IV cancerous animals were treated with the ethanolic extract of Oxalis corniculata Linn. One hundred and 400 mg/kg b.w. respectively, whereas Group V cancerous animals received standard drug cyclophosphamide 25 mg/kg b.w. The test and standard drugs were administered orally 24 hrs after the inoculation of EAC for 9 days. The several changes of antitumor potential of EEOC were accessed. Biochemical parameters such as total protein, albumin, Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP), lipid peroxidation and enzymatic antioxidants were determined. Results: The present study revealed that EEOC showed significant antitumor and antioxidant activities in EAC bearing mice. The dose dependent reduction in body weight, tumor volume, packed cell volume, tumor cell counts and increase in median survival time (MST) and percentage increase in life span in EEOC treated animals were observed. There was a significant increase in RBC count; Hb content in EEOC treated animals and reduction in total WBC count. There was a significant increase in total protein and albumin content, but decrease in AST, ALT and ALP contents in EEOC treated animals. A significant decrease in liver MDA levels and increase in catalase and reduced glutathione levels were observed in EEOC treated animals. Conclusion: Results conclude that the EEOC was effective in inhibiting the tumor growth in ascitic and solid tumor models. The biochemical and antioxidants studies were also supported its antitumor properties.

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