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Woburn, MA, United States

Huang C.-S.,University of Illinois at Urbana - Champaign | Chaudhery V.,University of Illinois at Urbana - Champaign | Pokhriyal A.,University of Illinois at Urbana - Champaign | George S.,University of Illinois at Urbana - Champaign | And 6 more authors.
Analytical Chemistry | Year: 2012

A photonic crystal (PC) surface is demonstrated as a high-sensitivity platform for detection of a panel of 21 cancer biomarker antigens using a sandwich enzyme-linked immunosorbent assay (ELISA) microarray format. A quartz-based PC structure fabricated by nanoimprint lithography, selected for its low autofluorescence, supports two independent optical resonances that simultaneously enable enhancement of fluorescence detection of biomarkers and label-free quantification of the density of antibody capture spots. A detection instrument is demonstrated that supports fluorescence and label-free imaging modalities, with the ability to optimize the fluorescence enhancement factor on a pixel-by-pixel basis throughout the microarray using an angle-scanning approach for the excitation laser that automatically compensates for variability in surface chemistry density and capture spot density. Measurements show that the angle-scanning illumination approach reduces the coefficient of variation of replicate assays by 20-99% compared to ordinary fluorescence microscopy, thus supporting reduction in limits of detectable biomarker concentration. Using the PC resonance, biomarkers in mixed samples were detectable at the lowest concentrations tested (2.1-41 pg/mL), resulting in a three-log range of quantitative detection. © 2011 American Chemical Society. Source


Huang C.-S.,406 West Green Street | George S.,University of Illinois at Urbana - Champaign | Lu M.,406 West Green Street | Lu M.,SRU Biosystems Inc. | And 5 more authors.
Analytical Chemistry | Year: 2011

We report on the use of photonic crystal surfaces as a high-sensitivity platform for detection of a panel of cancer biomarkers in a protein microarray format. The photonic crystal surface is designed to provide an optical resonance at the excitation wavelength of cyanine-5 (Cy5), thus providing an increase in fluorescent intensity for Cy5-labeled analytes measured with a confocal microarray scanner, compared to a glass surface. The sandwich enzyme-linked immunosorbent assay (ELISA) is undertaken on a microarray platform to undertake a simultaneous, multiplex analysis of 24 antigens on a single chip. Our results show that the resonant excitation effect increases the signal-to-noise ratio by 3.8- to 6.6-fold, resulting in a decrease in detection limits of 6-89%, with the exact enhancement dependent upon the antibody-antigen interaction. Dose-response characterization of the photonic crystal antibody microarrays shows the capability to detect common cancer biomarkers in the <2 pg/mL concentration range within a mixed sample. © 2011 American Chemical Society. Source


Zheng J.,University of Illinois at Urbana - Champaign | Ge C.,University of Illinois at Urbana - Champaign | Wagner C.J.,University of Illinois at Urbana - Champaign | Meng L.,SRU Biosystems Inc. | And 2 more authors.
IEEE Photonic Society 24th Annual Meeting, PHO 2011 | Year: 2011

We have demonstrated a tunable external cavity ring laser, in which the lasing wavelength can be controlled by external illumination that excites a nonlinear dye within a photonic crystal resonant reflector. © 2011 IEEE. Source


Trademark
X Body Inc. and SRU Biosystems Inc. | Date: 2007-01-30

Biomolecular screening sensors; optical scanners and high throughput screening instruments used to measure the output from biomolecular screening sensors; laboratory wellplates, namely, laboratory ware embedded with optical resonance structures used for biomolecular screening; holographic diffraction gratings used for biomolecular screening.


Trademark
X Body Inc. and SRU Biosystems Inc. | Date: 2008-11-25

Biomolecular screening sensors; optical scanners and high throughput screening instruments used to measure the output from biomolecular screening sensors; laboratory wellplates, namely, laboratory ware embedded with optical resonance structures used for biomolecular screening; holographic diffraction gratings used for biomolecular screening.

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