SRU Biosystems Inc.

Woburn, MA, United States

SRU Biosystems Inc.

Woburn, MA, United States
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Patent
SRU Biosystems Inc. | Date: 2010-04-13

The invention provides methods of detecting a change in cell growth patterns, methods of screening many different antibodies in one receptacle, and methods of detecting specific binding of an antibody to a protein or cell, wherein the antibody is in a mixture of many different antibodies.


Patent
SRU Biosystems Inc. | Date: 2011-11-02

The invention provides methods for detecting interactions between phage and antigen and antibody using biosensors.


An optical arrangement for illuminating a surface of a biosensor having a periodic surface grating structure. The arrangement includes a light source generating light, collimating optics for collimating the light from the light source, and a first reflecting surface (e.g., prism surface) receiving light from the collimating optics and directing incident light onto a surface of the biosensor and a second spatially separated reflecting surface receiving light reflected from the surface of the biosensor. The arrangement further includes telecentric optics (e.g., telecentric lens) receiving light from the second surface of the prism. The telecentric lens directs light onto an entrance slit of a spectrometer. The arrangement increases the light collection efficiency at the spectrometer as compared to prior art arrangements.


Patent
SRU Biosystems Inc | Date: 2011-11-08

The invention provides methods of detecting activation of an immune cell, methods for detecting blocking or enhancing properties of a test reagent or stimuli on activation of one or more immune cells or platelets, methods for selecting hybridomas producing antibodies to an antigen for quality of strength of binding to the antigen, methods for determining if a subject has had an immune response to an immunogen, methods of isolating neutralizing antibodies for an immunogen, methods of classifying a B cell lymphoma, and methods for selecting activated B-cells expressing an antibody to one or more antigens.


An instrument system for detecting biochemical interactions on a biosensor, said instrument system comprising:(a) a biosensor comprising:(i) a two-dimensional grating;(ii) a substrate that supports the two-dimensional grating; wherein the refractive index of the two-dimensional grating is greater than the refractive index of the substrate; and(iii) one or more specific binding substances immobilized on the surface of the two-dimensional grating opposite of the substrate; wherein, when the biosensor is illuminated a resonant grating effect is produced on a reflected or transmitted radiation spectrum of incident light by generation of a leaky mode in the grating; and wherein the depth and period of the two-dimensional grating are less than the wavelength of the resonant grating effect;(b) a light source;(c) a first optical fiber coupled to the light source, wherein the first optical fiber illuminates the biosensor;(d) a second optical fiber that detects a signal reflected or transmitted from the surface of the biosensor; and(e) a spectrometer for determining spectra of the reflected or transmitted signal from the biosensor.


Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters.


A grating-based biosensor comprising a substrate having a periodic surface grating structure, wherein the periodic grating structure is constructed in a manner designed for optical interrogation of the sensor with excitation light at at least two discrete wavelengths and polarizations in an evanescent resonance detection mode to thereby produce a luminescence response from a sample placed on the biosensor at at least two discrete emission wavelengths.


Patent
Sru Biosystems Llc | Date: 2010-05-18

Performing high-resolution determination of the relative shift of the spectral properties of a biosensor. The shift in the resonance peak of the biosensor is indicative of the amount of material bound to the surface of the biosensor. A preferred biosensor is a Guided Mode Resonant Filter Biosensor (GMRFB). In one aspect of the invention, curve fitting is used to determine the relative location of the spectrum of the unexposed biosensor with respect to those spectra that are altered (e.g., shifted) by the presence of materials bound to the surface of the biosensor. In an alternative embodiment, the cross correlation function is used to detect spectral peak offsets between a reference spectrum and a spectrum measured from an exposed biosensor. In yet another alternative, maximal likelihood estimation techniques are used to determine the spectral shift or offs.


A grating-based sensor is disclosed that has a grating structure constructed and designed for both evanescent resonance (ER) fluorescence detection and label-free detection applications. Some embodiments are disclosed which are optimized for ER detection in an air mode, in which the sample is dry. Other embodiments are optimized for ER detection in liquid mode, in which the sample is suspended in liquid medium such as water. One and two-dimensional gratings are also disclosed, including gratings characterized by unit cells with central posts, central holes, and two-level, two-dimensional gratings. A readout system for such sensors is also disclosed. One embodiment includes a first light source optimized for collecting label - free detection data, a second light source optimized for collecting ER fluorescence amplification data, and at least one detector. In one embodiment, the detector is an imaging system and includes a CCD camera for collecting both ER and label-free data. In other embodiments, the at least one detector takes the form of a spectrometer for collection of label-free data and a photomultiplier for collecting ER data. In other embodiments, a single light source such as a tunable laser or broad band light source is used.


Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters.

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