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Verma G.,SRL | Kuri G.C.,Sri Sankaradeva Nethralaya | Bhattacharjee H.,Sri Sankaradeva Nethralaya | Bharali G.,Sri Sankaradeva Nethralaya | And 3 more authors.
Indian Journal of Ophthalmology | Year: 2016

Immunoglobulin G4 (IgG4-related diseases) affects various tissues and organs of the human body. Orbital, adnexal, and scleral inflammations were already reported in the medical literature. To the best of our knowledge, we report the first case of intraocular IgG4-associated inflammatory mass in the ciliary body mimicking as a melanoma in a 23-year-old female from Northeast India. Characteristic histopathology, immunohistochemistry in the tissue, protein chemistry, and raised serum IgG4 were supportive for the diagnosis. As this newly diagnosed disease has multi-organ affection and little is known about its pathogenesis particularly in eye and adnexa, the present case will open many challenges in clinico-pathological diagnosis and research in the future.

Konishi Y.,SRL | Hayashi H.,SRL | Suzuki H.,Sapporo Medical University | Yamamoto E.,Sapporo Medical University | And 2 more authors.
Analytical Biochemistry | Year: 2015

Quantifying levels of DNA methylation in tumors is a useful approach for the identification of potential tumor suppressors and to find biomarkers that can be used as prognostic or therapeutic indicators. In the current study, we compared three methods commonly used for quantifying DNA methylation - bisulfite pyrosequencing, quantitative methylation-specific PCR (Q-MSP), and MethyLight - by focusing on the CpG island of the gene encoding the microRNA-34b and microRNA-34c (miR-34b/c); aberrant regulation of this miR is associated with various human malignancies, including gastric cancer. Standard curve analysis using control DNA samples demonstrated the highest quantitative accuracy in Q-MSP analysis. We also carried out methylation analysis using gastric mucosa specimens obtained from gastric cancer patients. We found a high correlation between methylation levels determined by Q-MSP and those determined by MethyLight (R2 = 0.952), whereas the results of bisulfite pyrosequencing and the other two methods were less well correlated (R2 = 0.864 and R2 = 0.804 for Q-MSP and MethyLight, respectively). This may reflect possible PCR bias in the pyrosequencing technique, which we show can be corrected for by applying a cubic approximate equation to the original data. Thus, although results obtained by the different DNA methylation analysis techniques are largely comparable, an appropriate correction may be necessary for stringent comparison. © 2015 Elsevier Inc. All rights reserved.

Gupta N.,Fortis Hospital | Sanchety N.,Fortis Hospital | Verma P.S.,Fortis Hospital | Verma G.,SRL
Indian Journal of Pathology and Microbiology | Year: 2015

Malignant granular cell tumor (MGCT) is rare tumors that comprise 1-2% of all granular cell tumors. They commonly arise on lower extremity, nuchal region, chest wall, gastrointestinal tract, head, and neck but very rarely in breast. We report a case of a MGCT of breast with review of literature. The patient had noticed a breast mass 4 years back which was operated, and wide local excision was done. The tumor was diagnosed as MGCT. The tumor fulfilled 3 of the 6 criteria of Fanburg-Smith et al. The patient received 8 cycles of chemotherapy thereafter with 4 cycles of antharacycline and 4 of taxanes. However, the tumor reoccurred 4 years after resection and grew rapidly. Contrast-enhanced computed tomography done showed a large lobulated breast mass with axillary lymph node metastasis. She underwent Modified Radical Mastectomy with axillary clearance. The histopathology this time also revealed similar malignant tumor. To the best of our knowledge, only 7 cases have been reported in indexed English literature occurring primarily in breast.

PubMed | SRL
Type: Journal Article | Journal: International journal of oncology | Year: 2011

To establish a rapid, specific, sensitive and simple assay method for doxorubicin (DXR) in body fluid, monoclonal antibodies (MAbs) against DXR were generated by immunizing mice with keyhole limpet hemocyanin-DXR conjugate, cell fusion, and a one step, time saving screening ELISA method using aminoplate-coupled DXR via a glutaraldehyde bridge as solid phase antigen. Inhibition ELISA for DXR-immunoassay was established using anti-DXR MAb of the best producer (2E9) and aminoplate-coupled DXR as antigen and DXR ranging from 50 pg to 50 ng in the body fluid or in the cell extract could be detected. MAb 2E9 cross-reacted to various degrees to anthracycline compounds, such as some DXR analogues and derivatives, but did not recognize anthracene and anthraquinone structures.

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