Sri Venkateswara Veterinary University
Tirupati, India
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Kumari K.N.,Sri Venkateswara Veterinary University
Comparative Clinical Pathology | Year: 2014

In the present study, haemato-biochemical changes associated with canine demodicosis are reported. Dogs (n, 16) affected with generalized demodicosis revealed significant reduction in total erythrocyte count and haemoglobin levels. All the dogs had normal packed cell volume. Affected dogs also showed leukocytosis accompanied by neutrophilia, eosinophilia and lymphopenia. Dogs had normal serum protein levels, normal A/G ratio, reduced serum albumin levels, increased serum globulin and cholesterol levels. Dogs with demodicosis did not show any significant difference in total T4 and free T4 levels when compare with health dogs. © 2014 Springer-Verlag London.

Rao D.S.T.,Sri Venkateswara Veterinary University
International Journal of Pharma and Bio Sciences | Year: 2012

Of 320 buffaloes within 60 days after calving, with the history of reduced feed intake and decreased milk yield presented, 90 (28.13%) buffaloes were found suffering with PPI. On detailed clinical examination and urinalysis, it was observed that 43 (47.78%) buffaloes had hepatic insufficiency. Out of 43 buffaloes of hepatic insufficiency, 23 (25.56 %) had hepatic insufficiency alone. Urine samples were positive for bile pigments. Serum samples were analysed for glucose, calcium, phosphorus, magnesium, total bilirubin, cholesterol, AST, GGT, total protein and albumin and compared with control buffaloes. Buffaloes with hepatic insufficiency were randomly divided in to two different groups. One group (n=11) animals were given Rintose at the rate of 0.5g/ kg b wt iv for one day and inj. Livadex Forte 10 ml im for 3 days. The second group (n=12) animals were given Rintose at the rate of 0.5g/ kg b wt iv for one day and inj. Toxol 10 ml im for 3 days. There was significant improvement in serum glucose, AST, GGT and calcium levels after the treatments and the therapeutic efficacy was found to be same.

Reddy G.V.B.,Sri Venkateswara Veterinary University | Sen A.R.,National Research Center on Meat | Nair P.N.,National Research Center on Meat | Reddy K.S.,Sri Venkateswara Veterinary University | And 2 more authors.
Meat Science | Year: 2013

The antioxidant and antimicrobial efficacy of grape seed extract (GSE) was studied in restructured mutton slices (RMS) under aerobic and vacuum packaging conditions during refrigerated storage. The RMS treated with grape seed extract (GSE) had significantly (P. <. 0.05) lower thiobarbituric acid reactive substance (TBARS) values and free fatty acids (FFA) % compared to control (C) and butylated hydroxy anisole (BHA) treated RMS during storage at 4. ±. 1. °C. Addition of GSE significantly (P. <. 0.05) reduced the total psychrophilic and coliform counts in RMS during refrigerated storage. The GSE treated mutton slices recorded significantly (P. <. 0.05) superior scores of color, flavor, juiciness and overall palatability than C and BHA treated RMS. The TBARS values, FFA % and microbial counts increased significantly (P. <. 0.05) during storage. It can be concluded that GSE has excellent antioxidant and antimicrobial properties compared to control and BHA treated RMS during refrigerated storage under aerobic and vacuum conditions. © 2013 Elsevier Ltd.

Devasena B.,Sri Venkateswara Veterinary University | Adilaxmamma K.,Sri Venkateswara Veterinary University
Buffalo Bulletin | Year: 2016

Present study was undertaken in order to study Delonix elata (L) Gamble for its nutritive value so as to use this particular tree leaves as livestock feed. The findings shown that, Delonix elata (L) Gamble contained, 32.8, 90.9, 25.2, 2.4, 18.2, 9.1, and 45.1% of DM, OM, CP, EE, CF, TA and NFE, respectively. The cell wall constituents (%) were, 46.5 (NDF), 24.3 (ADF), 22.2 (hemicellulose) and 5.3 (ADL). The determination of different fraction of carbohydrates and protein as per CNCPS (Cornell Net Carbohydrate and Protein System) revealed that, carbohydrate fraction A and protein fraction B2 contents were more, indicating the readily available nutrients are more in this forage. The IVDMD and IVOMD were 76.7 and 77.4%, respectively. In sacco DM degradability indicated readily soluble component a-14.3, insoluble but degradable component, b-68.7, rate constant at which ‘b’ is degraded c- 0.0586 and effective degradable dry matter (EDDM) of 55.8%. While the protein degradability study indicated a-18.3, b-60.4, c- 0.0904 and effective degradable protein (EDP) was 60.8%. The total tannin content was 4.1 g%. It can be concluded that, Delonix elata can be used as livestock feed. © 2016, Kasetsart University. All rights reserved.

Reddy B.S.,Sri Venkateswara Veterinary University | Sivajothi S.,Sri Venkateswara Veterinary University
Comparative Clinical Pathology | Year: 2016

Malassezia pachydermatis is the most common causative organism for development of Malassezia dermatitis in dogs. An adult pug was presented to the clinic with chronic skin abnormalities, rancid odour from the body and pruritus. Lesion distribution was noticed on the ventral chin, neck, abdomen and inner surface of the legs. Skin scrapings, hair plucks and tape impression smears were examined, and footprint-shaped Malassezia organisms were identified in impression smears. Blood was collected to diagnose underlying factor for development of chronic recurrent Malassezia dermatitis. Up on blood tests, lowered total T4 (2.02 μg/dL) and free T4 (0.68 ng/dL) were detected. Treatment was given with oral ketoconazole (5-mg/kg body weight) resulted in improvement of clinical signs within two weeks of therapy. Thyroxine (at 20-μg/kg body weight BID, PO) was administered, and thyroxine levels in blood were in between the normal range after 2 months. No recurrence of dermatitis and pruritus was observed during the observatory period of six months. © 2015, Springer-Verlag London.

Reddy B.S.,Sri Venkateswara Veterinary University | Sivajothi S.,Sri Venkateswara Veterinary University
Comparative Clinical Pathology | Year: 2016

Canine generalised demodicosis is recognised as a severe skin disease of dogs, and studies on CD4+ and CD8+ T cells in this disease condition have been limited. The aim of the present study was to record the CD4+ and CD8+ T cells in the peripheral blood of dogs affected with generalised demodicosis. Whole blood was collected from dogs with generalised demodicosis for estimation of CD4+ and CD8+ T cells by flow cytometry. The demodicosis-affected dogs had lower number of CD4+ T cells, increased CD8+ T cells, and reduced CD4+/CD8+ ratio compared to healthy controls. Present abnormalities in the study are related to the immunological response of the dogs to the mites in a disease process. © 2015, Springer-Verlag London.

Nagalakshmi D.,Sri Venkateswara Veterinary University | Dhanalakshmi K.,Sri Venkateswara Veterinary University | Himabindu D.,Sri Venkateswara Veterinary University
Small Ruminant Research | Year: 2011

An experiment was conducted with 21 Nellore male lambs (9-10 months age) of uniform body weight (27.6±1.03kg) to assess the effect of replacing groundnut cake (GNC) totally with sunflower seed cake (SFC) and karanj seed cake (KSC) in sorghum stover-based complete diets on growth performance, nutrient utilisation, immune response and carcass traits. Lambs were randomly assigned to a control diet (13.0% GNC) and two test diets containing SFC (14.5%) and KSC (12.0%), as sole protein supplements and fed respective diets according to requirements recommended by the Indian Council of Agricultural Research, 1998 for a period of 155 days. Daily feed intake and fortnightly body weight of each lamb were recorded throughout the feeding trial. After 120 days, a metabolic trial of 6 days was conducted to determine nutrient digestibilities and balances. After the 75th day, all lambs were sensitised intramuscularly with heat-killed Brucella abortus S99 and 20% chicken red blood cell (RBC) suspension and a booster was given after 15 days to assess the humoral immune response. Antibody titres were estimated in serum collected from sensitised lambs on 7, 14, 21, 28 and 35th day post sensitisation. The cell-mediated immune response was assessed by delayed type hypersensitive (DTH) reaction against phytohemagglutinin-P and in vitro lymphocyte proliferative assay (LPA) on the 150th day of the experiment. On the 155th day, four representative lambs from each group were slaughtered for carcass studies. The karanjin content in KSC estimated by high-performance liquid chromatography (HPLC) was 0.325%, hence constituted 390mg karanjin per kg diet. Dry matter (DM), nutrient intake and digestibilities of proximate constituents, energy and fibre fractions by SFC- and GNC-fed lambs did not differ, but DM and nutrient intake by lambs which were fed KSC was lowered (P<0.05) compared to other diets. This resulted in lower (P<0.05) growth rates, nutrient efficiency and relatively higher digestibility co-efficients for DM, organic matter, crude fat, nitrogen-free extract, energy and neutral detergent fibre in KSC-fed lambs. Balance of phosphorus (P) was comparable among the groups, but calcium (Ca) and nitrogen (N) balance was lower (P<0.05) in KSC-fed lambs. Antibody titres against B. abortus and chicken RBC was depressed (P<0.05) in lambs which were fed KSC diet on the 7th day post sensitisation, while such depression was not observed with SFC feeding. The DTH response and in vitro LPA response were not affected by SFC, but the response was lower on KSC diet. No effect of variation in protein supplements was observed on carcass characteristics. The study indicated that the incorporation of SFC as the sole protein source in the diets of lambs had no effect on body weight, nutrient intake, nutrient digestibilities, immune response and carcass traits. But with 12% KSC inclusion in complete diets (390mg karanjin per kg), there was depressed performance and immunocompetence in lambs. © 2011 Elsevier B.V.

Gangagni Rao A.,Indian Institute of Chemical Technology | Surya Prakash S.,Indian Institute of Chemical Technology | Joseph J.,Indian Institute of Chemical Technology | Rajashekhara Reddy A.,Sri Venkateswara Veterinary University | Sarma P.N.,Indian Institute of Chemical Technology
Bioresource Technology | Year: 2011

A multi stage high rate biomethanation process with novel self mixed anaerobic digester (SMAD) was developed in the present study to reduce the hydraulic residence time (HRT), increase the volatile solids (VS) loading rate, improve the VS destruction efficiency and enhance the methane yield. Specific design features of SMAD were useful in mixing the digester contents without consuming power and de-alienated the problem of scum formation. In the first phase, poultry litter having 10% total solids (TS) was subjected to high rate biomethanation in multi stage configuration (SMAD-I and II in series with UASB reactor). It was observed that gross VS reduction of 58%, gross methane yield of 0.16m3kg-1 (VS reduced) and VS loading rate of 3.5kgVSm-3day-1 at HRT of 13days was obtained. In the second phase SMAD-II was bypassed from the process scheme keeping the other parameters same as in the first phase. The results obtained were not as encouraging as in the first phase. The study showed that multi stage configuration with SMAD design improved the anaerobic digestion process efficiency of poultry litter. © 2010 Elsevier Ltd.

Sivajothi S.,Sri Venkateswara Veterinary University | Reddy B.S.,Sri Venkateswara Veterinary University
Comparative Clinical Pathology | Year: 2015

Sarcoptes scabiei infestation in cats was rare. A 1-year-old tom cat was presented to the clinic with recurrent dermatitis, emaciation, severe itching and presence of skin lesions over the face, forehead and ears. Microscopic examination of skin scrapings from the skin lesions revealed the presence of mites. Based on the morphology of the mites, sarcoptic mange was confirmed. Cat was treated with parenteral ivermectin and supportive therapy. Complete recovery was observed by the fifteenth day of treatment. © 2014, Springer-Verlag London.

Mutha Rao M.,Sri Venkateswara Veterinary University | Uma Mahesh Y.,Sri Venkateswara Veterinary University
Buffalo Bulletin | Year: 2012

A study was undertaken to assess the comparative efficacy of three harvesting techniques viz., the aspiration, puncture and slicing methods on oocyte recovery in buffalo ovaries obtained from a local abattoir. Effect of ovarian status (luteal phase vs. non-luteal phase), ovarian volume (<1.5 cm3 vs. >1.5 cm 3), side of ovary (right vs. left) and number of visible follicles (No follicles vs.1-5 follicles vs. 6-10 follicles) on oocyte yield and quality were also analyzed. Among the three collection methods, the slicing technique yielded the highest number of total and good quality oocytes, respectively (7.98±0.70 and 3.23±0.30) followed by the puncture (3.46±0.31 and 1.25±0.17) and the aspiration methods (2.38±0.19 and 0.84±0.10). Irrespective of the method employed, a little over 1/3 of recovered oocytes were of poor quality (32.46-34.34%). Luteal phase ovaries (having CL) yielded lower numbers of oocytes compared to non-luteal phase (no CL) ovaries. Both left and right ovaries contributed equally to total as well as different quality grades of oocytes in all the methods. Ovarian volume non-significantly affected the oocyte yield, which was slightly higher in ovaries with a mean volume >1.5 cm3. The results indicate that slicing method is superior to the other two methods employed in this study to harvest a greater number of good quality as well as culture grade oocytes from buffalo ovaries.

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