Sri Venkateswara Veterinary University
Tirupati, India
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Kumari K.N.,Sri Venkateswara Veterinary University
Comparative Clinical Pathology | Year: 2014

In the present study, haemato-biochemical changes associated with canine demodicosis are reported. Dogs (n, 16) affected with generalized demodicosis revealed significant reduction in total erythrocyte count and haemoglobin levels. All the dogs had normal packed cell volume. Affected dogs also showed leukocytosis accompanied by neutrophilia, eosinophilia and lymphopenia. Dogs had normal serum protein levels, normal A/G ratio, reduced serum albumin levels, increased serum globulin and cholesterol levels. Dogs with demodicosis did not show any significant difference in total T4 and free T4 levels when compare with health dogs. © 2014 Springer-Verlag London.

Ayodhya S.,Sri Venkateswara Veterinary University
Journal of Advanced Veterinary and Animal Research | Year: 2014

The present study was carried out during the month of January 2014 when a total of 148 dogs with history of various diseases were presented to the Campus Veterinary Hospital, Teaching Veterinary Clinical Complex, College of Veterinary Science, Rajendranagar, Hyderabad, India. Out of 148 dogs that were presented to the hospital, 48 dogs had the clinical signs of loss of hair, itching, and reduced food intake. The dogs were restless and continuously rubbed their bodies against the walls in the houses, and scratching with their legs. Clinical examination of the dogs revealed presence of alopecia, pruritus, and the formation of small crusts. All 48 dogs were treated with ivermectin by subcutaneous injection dosed at 0.02 mL/kg body weight at a weekly interval for 2 to 3 weeks. All dogs were bathed with cypermethrin shampoo weekly once for 2-3 weeks. In the present study, it was observed that ivermectin/cypermethrin combination therapy was effective for the management of tick infestation in dogs.

Rao P.P.,Ella Foundation | Reddy Y.N.,Sri Venkateswara Veterinary University | Ganesh K.,Genotypic Technology and 259 | Nair S.G.,Genotypic Technology and 259 | And 2 more authors.
Journal of Virological Methods | Year: 2013

Bluetongue (BT) is an economically important endemic disease of livestock in tropics and subtropics. In addition, its recent spread to temperate regions like North America and Northern Europe is of serious concern. Rapid serotyping and characterization of BT virus (BTV) is an essential step in the identification of origin of the virus and for controlling the disease. Serotyping of BTV is typically performed by serum neutralization, and of late by nucleotide sequencing. This report describes the near complete genome sequencing and typing of two isolates of BTV using Illumina next generation sequencing platform. Two of the BTV RNAs were multiplexed with ten other unknown samples. Viral RNA was isolated and fragmented, reverse transcribed, the cDNA ends were repaired and ligated with a multiplex oligo. The genome library was amplified using primers complementary to the ligated oligo and subjected to single and paired end sequencing. The raw reads were assembled using a de novo method and reference-based assembly was performed based on the contig data. Near complete sequences of all segments of BTV were obtained with more than 20× coverage, and single read sequencing method was sufficient to identify the genotype and serotype of the virus. The two viruses used in this study were typed as BTV-1 and BTV-9E. © 2013 Elsevier B.V.

Sreenivas D.,Sri Venkateswara Veterinary University
Veterinary World | Year: 2013

Dairy cattle and buffalo production has undergone a major transformation resulting into a substantial increase in milk production thus helping our country to achieve top position in milk production in the world. Even though various animal breeding and managementprogrammes have contributed to this improvement, there are constraints hampering the growth which require proper attention by initiating realistic breeding policies for genetic improvement of cattle and buffaloes. In view of this, optimum breeding policies for various classes of cattle and buffaloes are presented with their strengths and shortcomings. Technologies for selection of superior genotypes and multiplication of their germplasm are also being discussed. Finally, some breeding policy strategies for proper implementation of these programmes are recommended for adoption.

Nagalakshmi D.,Sri Venkateswara Veterinary University | Narsimha Reddy D.,Sri Venkateswara Veterinary University
Animal Nutrition and Feed Technology | Year: 2010

A complete diet was formulated with sugarcane bagasse as sole roughage source (28.5%) and processed with expander-extruder. The extruded diet was compared with a traditional system of feeding comprising concentrate mixture, ad libitum chopped sorghum straw and limited quantity of green hybrid Napier (10kg/animal/day) in terms of nutrient utilization and milk characteristics in 12 lactating Murrah buffaloes (6 in each group) for 180 days. The buffaloes consumed less (P<0.01) dry matter (DM) per kg metabolic body weight when fed processed complete diet than those fed conventional ration. However, the DM intakes in both the groups were higher than the standard recommended requirements (Kearl, 1982). The water intake/kg DM intake was higher (P<0.01) in expander extruder processed complete diet fed animals. Blending of bagasse with concentrates and expander extruder processing complete diet resulted in higher (P<0.01) crude protein digestibility. The digestibility of other nutrients and fibre fractions remained comparable among the groups. The digestible crude protein of complete ration was higher (P<0.05) than the conventional ration. The higher (P<0.01) intakes of total digestible nutrients and metabolizable energy by buffaloes fed conventional ration was a reflection of more roughage consumption (61.9% Vs 28.5%) by these animals. Milk yield, fat corrected milk (FCM) yield, fat and SNF % was similar between both the groups. The DMI/kg FCM production was significantly (P<0.05) lower on processed complete diet compared to conventional diet. The cost of feeding/kg milk production was lower (P<0.01) and reduced by 31.53% when expander extruder pellet complete diet containing sugarcane bagasse was fed to animals.

Eevuri T.R.,Sri Venkateswara Veterinary University | Putturu R.,Sri Venkateswara Veterinary University
Veterinary World | Year: 2013

The importance and use of herbal remedies (turmeric, tulsi, amla and aloe vera etc.) has been reviewed systematically. Turmeric (curcumin), acts as an antioxidant, antimutagenic, antiinflamatory and antimicrobial agent and protect liver against a variety of toxicants. Tulsi (eugenol) have anticancer properties, reduced blood glucose levels, total cholesterol levels and promotes immune system function. Amla, richest source of vitamin-c and it's active tannoid principles have antimicrobial, antidiabetics, anticarcinogenic properties and enhances immune property. Aloe vera contains phytochemicals (Saponins, flavonoids, alkaloids and phenols), which is an indication of cosmetic and medicinal value. Turmeric, tulsi, amla and aloe vera preparations increased the body weight gain, feed efficiency and decreased the feed intake. These preparations decreased the mortality rates and the cost of feed has been decreased from 6.2% to 13.5%. They have reduced the fat accumulation, increased dressing percentage, liver weight, spleen weight and whole giblet weights. Significant reduction of serum cholesterol, serum triglycerides and increased the humoral response against RD vaccine.

Nagalakshmi D.,Sri Venkateswara Veterinary University | Dhanalakshmi K.,Sri Venkateswara Veterinary University | Himabindu D.,Sri Venkateswara Veterinary University
Small Ruminant Research | Year: 2011

An experiment was conducted with 21 Nellore male lambs (9-10 months age) of uniform body weight (27.6±1.03kg) to assess the effect of replacing groundnut cake (GNC) totally with sunflower seed cake (SFC) and karanj seed cake (KSC) in sorghum stover-based complete diets on growth performance, nutrient utilisation, immune response and carcass traits. Lambs were randomly assigned to a control diet (13.0% GNC) and two test diets containing SFC (14.5%) and KSC (12.0%), as sole protein supplements and fed respective diets according to requirements recommended by the Indian Council of Agricultural Research, 1998 for a period of 155 days. Daily feed intake and fortnightly body weight of each lamb were recorded throughout the feeding trial. After 120 days, a metabolic trial of 6 days was conducted to determine nutrient digestibilities and balances. After the 75th day, all lambs were sensitised intramuscularly with heat-killed Brucella abortus S99 and 20% chicken red blood cell (RBC) suspension and a booster was given after 15 days to assess the humoral immune response. Antibody titres were estimated in serum collected from sensitised lambs on 7, 14, 21, 28 and 35th day post sensitisation. The cell-mediated immune response was assessed by delayed type hypersensitive (DTH) reaction against phytohemagglutinin-P and in vitro lymphocyte proliferative assay (LPA) on the 150th day of the experiment. On the 155th day, four representative lambs from each group were slaughtered for carcass studies. The karanjin content in KSC estimated by high-performance liquid chromatography (HPLC) was 0.325%, hence constituted 390mg karanjin per kg diet. Dry matter (DM), nutrient intake and digestibilities of proximate constituents, energy and fibre fractions by SFC- and GNC-fed lambs did not differ, but DM and nutrient intake by lambs which were fed KSC was lowered (P<0.05) compared to other diets. This resulted in lower (P<0.05) growth rates, nutrient efficiency and relatively higher digestibility co-efficients for DM, organic matter, crude fat, nitrogen-free extract, energy and neutral detergent fibre in KSC-fed lambs. Balance of phosphorus (P) was comparable among the groups, but calcium (Ca) and nitrogen (N) balance was lower (P<0.05) in KSC-fed lambs. Antibody titres against B. abortus and chicken RBC was depressed (P<0.05) in lambs which were fed KSC diet on the 7th day post sensitisation, while such depression was not observed with SFC feeding. The DTH response and in vitro LPA response were not affected by SFC, but the response was lower on KSC diet. No effect of variation in protein supplements was observed on carcass characteristics. The study indicated that the incorporation of SFC as the sole protein source in the diets of lambs had no effect on body weight, nutrient intake, nutrient digestibilities, immune response and carcass traits. But with 12% KSC inclusion in complete diets (390mg karanjin per kg), there was depressed performance and immunocompetence in lambs. © 2011 Elsevier B.V.

Gangagni Rao A.,Indian Institute of Chemical Technology | Surya Prakash S.,Indian Institute of Chemical Technology | Joseph J.,Indian Institute of Chemical Technology | Rajashekhara Reddy A.,Sri Venkateswara Veterinary University | Sarma P.N.,Indian Institute of Chemical Technology
Bioresource Technology | Year: 2011

A multi stage high rate biomethanation process with novel self mixed anaerobic digester (SMAD) was developed in the present study to reduce the hydraulic residence time (HRT), increase the volatile solids (VS) loading rate, improve the VS destruction efficiency and enhance the methane yield. Specific design features of SMAD were useful in mixing the digester contents without consuming power and de-alienated the problem of scum formation. In the first phase, poultry litter having 10% total solids (TS) was subjected to high rate biomethanation in multi stage configuration (SMAD-I and II in series with UASB reactor). It was observed that gross VS reduction of 58%, gross methane yield of 0.16m3kg-1 (VS reduced) and VS loading rate of 3.5kgVSm-3day-1 at HRT of 13days was obtained. In the second phase SMAD-II was bypassed from the process scheme keeping the other parameters same as in the first phase. The results obtained were not as encouraging as in the first phase. The study showed that multi stage configuration with SMAD design improved the anaerobic digestion process efficiency of poultry litter. © 2010 Elsevier Ltd.

Sivajothi S.,Sri Venkateswara Veterinary University | Reddy B.S.,Sri Venkateswara Veterinary University
Comparative Clinical Pathology | Year: 2015

Sarcoptes scabiei infestation in cats was rare. A 1-year-old tom cat was presented to the clinic with recurrent dermatitis, emaciation, severe itching and presence of skin lesions over the face, forehead and ears. Microscopic examination of skin scrapings from the skin lesions revealed the presence of mites. Based on the morphology of the mites, sarcoptic mange was confirmed. Cat was treated with parenteral ivermectin and supportive therapy. Complete recovery was observed by the fifteenth day of treatment. © 2014, Springer-Verlag London.

Mutha Rao M.,Sri Venkateswara Veterinary University | Uma Mahesh Y.,Sri Venkateswara Veterinary University
Buffalo Bulletin | Year: 2012

A study was undertaken to assess the comparative efficacy of three harvesting techniques viz., the aspiration, puncture and slicing methods on oocyte recovery in buffalo ovaries obtained from a local abattoir. Effect of ovarian status (luteal phase vs. non-luteal phase), ovarian volume (<1.5 cm3 vs. >1.5 cm 3), side of ovary (right vs. left) and number of visible follicles (No follicles vs.1-5 follicles vs. 6-10 follicles) on oocyte yield and quality were also analyzed. Among the three collection methods, the slicing technique yielded the highest number of total and good quality oocytes, respectively (7.98±0.70 and 3.23±0.30) followed by the puncture (3.46±0.31 and 1.25±0.17) and the aspiration methods (2.38±0.19 and 0.84±0.10). Irrespective of the method employed, a little over 1/3 of recovered oocytes were of poor quality (32.46-34.34%). Luteal phase ovaries (having CL) yielded lower numbers of oocytes compared to non-luteal phase (no CL) ovaries. Both left and right ovaries contributed equally to total as well as different quality grades of oocytes in all the methods. Ovarian volume non-significantly affected the oocyte yield, which was slightly higher in ovaries with a mean volume >1.5 cm3. The results indicate that slicing method is superior to the other two methods employed in this study to harvest a greater number of good quality as well as culture grade oocytes from buffalo ovaries.

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