Sri Vasavi Institute of Pharmaceutical science

Tādepallegūdem, India

Sri Vasavi Institute of Pharmaceutical science

Tādepallegūdem, India

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Pedaprolu J.N.L.,Sri Vasavi Institute of Pharmaceutical science | Bonthu M.G.,Sri Vasavi Institute of Pharmaceutical science | Vatchavai B.R.,Sri Vasavi Institute of Pharmaceutical science | Kamatham S.S.,Sri Vasavi Institute of Pharmaceutical science | And 2 more authors.
Eurasian Journal of Analytical Chemistry | Year: 2017

A stability-indicating RP-HPLC method was developed and validated for the estimation of Liraglutide in bulk and pharmaceutical dosage forms. Shiseido C18 (250 mm x 4.6 mm I.D., 5 μm particle size) column was used as stationary phase with mobile phase consisting methanol+acetonitrile (80:20): phosphate buffer (pH 3.0 adjusted with ortho phosphoric acid) in the ratio of 75:25, v/v. The flow rate was maintained at 1.2 ml/min and effluents were monitored at 245 nm. The retention time was found to be 2.837 minutes. The forced degradation studies were performed as per ICH guidelines under acidic, alkali, oxidative, thermal, photostability and neutral conditions. The drug peak was well resolved from the peaks of degraded products. From the degradation studies it is evident that the drug showed instability under acidic, alkali, oxidative, thermal, photostability and neutral conditions. The linearity of the method was observed in the concentration range of 10-60 μg/ml with the number of theoretical plates & tailing factor being 5550 & 1.17 respectively with a correlation coefficient of 0.999. The percentage assay of Liraglutide was found to be 99.66%. The method was validated for its accuracy, precision and system suitability. The results obtained in the study were within the limits of ICH guidelines and hence this method can be used for the estimation of Liraglutide in bulk and pharmaceutical dosage forms. © Authors.


Mohan Gandhi B.,Sri Vasavi Institute of Pharmaceutical science | Lakshmana Rao A.,Vv Institute Of Pharmaceutical Science
Oriental Journal of Chemistry | Year: 2016

Novel HPTLC and stability indicating RP-HPLC methods were developed for simultaneous estimation of Moxifloxacin (MOX) and Dexamethasone (DEX) in ophthalmic dosage form. For HPTLC method, the separation was carried out on HPTLC aluminum plates using acetonitrile:water:ammonia (8:1:0.5 V/V/V) as mobile phase and developed plates were read at 266 nm. The drugs were resolved satisfactorily with Rf values of 0.09±0.01 and 0.74±0.01 for MOX and DEX, respectively. The RP-HPLC analysis is carried out on Shiseido C18 column (250 mm x 4.6 mm I.D., 5 μm), using 0.02M acetate buffer (pH is 4 adjusted with triethylamine) and acetonitrile in the ratio of 60:40 V/V with a flow rate of 1.2 m/min and the detection was carried out at 254 nm. The retention times were found to be 2.144±0.5 min and 4.732±0.5 min. for MOX and DEX respectively. Developed methods were validated as per ICH guidelines and were found to be within the limits. © 2016, Oriental Scientific Publishing Company. All rights reserved.


Thangavel N.,Sri Vasavi Institute of Pharmaceutical science | Gupta J.K.,Jadavpur University
E-Journal of Chemistry | Year: 2010

The antioxidant potency of various extracts of Andrographis stenophylla leaf was evaluated in vitro using ferric thiocyanate method. Reductive ability and free radical scavenging activity of the extracts were also investigated. Amounts of phenolic compounds in each of the extracts were determined using Folin-Ciocalteau reagent and compared to observe the correlation between antioxidant activities and total phenolic content. Methanol extract exhibited maximum antioxidant activity and was found to contain 2% of total phenolic compounds. Methanol extract was subjected to column chromatographic separation over silica gel G using ethyl acetate: formic acid: acetic acid: water. Fractions thus obtained were screened for their antioxidant activity. Among the eleven fractions screened, fraction C was more active than the standard butylated hydroxyanisole. Fraction C on further fractionation with n-butanol: acetic acid: water afforded two flavanoids namely acacetine and isosakuranetine. Fraction A was also shown to possess good antioxidant activity which was developed using TLC and indicated the presence of a terpenoid, Andrographolide. The structures of the isolated compounds were confirmed by UV, IR, MS, 1H and 13C NMR spectral data. This is the first report wherein Andrographolide, Acacetine and Isosakuranetine are isolated from Andrographis stenophylla leaf.


Tripathy S.,Utkal University | Pradhan D.,Utkal University | Anjana M.,Sri Vasavi Institute of Pharmaceutical Science
International Journal of Pharma and Bio Sciences | Year: 2010

The anti arthritic potential of Ammania baccifera Linn (Lythraceae) extracts were evaluated by taking chronic inflammatory models like cotton pallet induced granuloma and complete Freund's Adjuvant (CFA)induced arthritis in albino rats. Arthritis was induced by injecting 0.1ml of complete Freund's adjuvant below the plantar aponeurosis of the right hind paw. Treatment with the extracts and standard started on the day of induction of inflamogens and continue up to 28 days. In this study both the alcoholic and aqueous extracts significantly (p<0.01) decrease the paw edema on 28 th day. The extracts also significantly rectified the deranged hematological parameters. In cotton pallet granuloma inflammation was induced by implanting the sterilized pre weighed cotton pallet subcutaneous in the ventral region of the groin. Treatment continued up to 8 day. The decrease in weight of the cotton pallet as compared to the control was considered as anti-inflammatory effect of the extracts. In both the inflammatory models alcoholic extracts show more potency then the aqueous extracts in terms of percentage of inhibition of inflammation.


Latha Y.B.M.,Sri Vasavi Institute of Pharmaceutical science | Gowri Sankar D.,Andhra University
Asian Journal of Pharmaceutical and Clinical Research | Year: 2013

A simple, rapid and accurate and stability indicating RP-HPLC method was developed for the determination of ramipril in pure and tablet forms. The method showed a linear response for concentrations in the range of 100-500 μg/mL using Acetonitrile: Buffer solution in the ratio (70:30) as the mobile phase with detection at 225 nm and a flow rate of 0.8 mL/min and retention time 2.287min. The value of correlation coefficient, slope and intercept were, 0.999, 9318.72and179702, respectively. The method was validated for precision, recovery, ruggedness and robustness. The drug undergoes degradation under acidic, basic, peroxide and thermal degradation conditions. All the peaks of degraded product were resolved from the active pharmaceutical ingredient with significantly different retention time. As the method could effectively separate the drug from its degradation product, it can be employed as a stability indicating one.


Bhaskara Raju V.,Sri Vasavi Institute of Pharmaceutical science | Lakshmana Rao A.,VV Institute of Pharmaceutical science
Rasayan Journal of Chemistry | Year: 2011

An accurate and precise RP-HPLC method was developed for the determination of perindopril in tablet dosage forms. Separation of die drug was achieved on a reverse phase Cig column using a mobile phase consisting of phosphate buffer and acetonitrile in the ratio of 65:35 v/v. The flow rate was 0.6 ml/min and die detection wavelength was 209 nm. The linearity was observed in the range of 20-100 ug/ml with a correlation coefficient of 0.9997. The proposed method was validated for its linearity, accuracy, precision and robustness. This method can be employed for routine quality control analysis of perindopril in tablet dosage forms. © 2011 RASAYAN.


Raju V.B.,Sri Vasavi Institute of Pharmaceutical science | Rao A.L.,Vv Institute Of Pharmaceutical Science
E-Journal of Chemistry | Year: 2012

An accurate and precise HPLC method was developed for the determination of lisinopril. Separation of the drug was achieved on a reverse phase C 8 column using a mobile phase consisting of phosphate buffer and methanol in the ratio of 35:65v/v. The flow rate was 0.8 mL/min and the detection wavelength was 215 nm. The linearity was observed in the range of 20-60 μg/mL with a correlation coefficient of 0.9992. The proposed method was validated for its linearity, accuracy, precision and robustness. This method can be employed for routine quality control analysis of lisinopril in tablet dosage forms.


Sahoo P.K.,Sri Vasavi Institute of Pharmaceutical science | Behera P.,JKK Nataraja Dental College and Hospital
European Journal of Medicinal Chemistry | Year: 2010

A series of novel ketoprofen derivatives 4a-j bearing both amide and carbamate functionalities were prepared using benzotriazole. Selective reduction of ketoprofen produced hydroxy derivative 2, which reacts with one or 2 mol of 1-benzotriazole carboxylic acid chloride (1) gave benzotriazole derivatives 3a and 3b respectively. Antioxidative screenings revealed that the prepared compounds 3b and 4a-j possess excellent lipid peroxidation inhibition at 0.1 mM concentration. Two of the compounds 3b and 4g also showed high soybean lipoxygenase inhibition activity, where as the amidocarbamate derivatives of ketoprofen showed only weak reducing activity against 1,1-diphenyl-2- picrylhydrazyl radicals. No selective antiviral effects were noted for the tested compounds against a broad variety of DNA and RNA viruses. © 2010 Elsevier Masson SAS.


Sahoo P.K.,Sri Vasavi Institute of Pharmaceutical science | Behera P.,JKK Nataraja Dental College and Hospital
European Journal of Medicinal Chemistry | Year: 2010

The acetic acid derivatives of [1,2,4]triazino[4,3-a]benzimidazole (TBI) were synthesized and tested in vitro and in vivo as selective aldose reductase (ALR2) inhibitors. Compound PS11 showed highest inhibitory activity (IC50) 0.32 μM and was found to be effective in preventing cataract development in severely galactosemic rats when administered as an eyedrop solution. All the compounds investigated were selective for ALR2, since none of them inhibited appreciably aldehyde reductase, sorbitol dehydrogenase, or glutathione reductase. © 2009 Elsevier Masson SAS. All rights reserved.


Raju V.B.,Sri Vasavi Institute of Pharmaceutical science | Rao A.L.,Vallabhaneni Venkatadri Institute of Pharmaceutical science
Asian Journal of Chemistry | Year: 2012

A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous estimation of moexipril and hydrochlorthiazide in tablets. A column having 150 mm × 4.6 mm i.d. in isocratic mode with mobile phase containing acetonitrile:phosphate buffer (45:55; adjusted to pH 3) was used. The flow rate was 0.6 mL/min and effluent was monitored at 215 nm. The retention time (min) and linearity range (μg/mL) for moexipril and hydrochlorthiazide were (4.294, 3.368) and (20-60, 20- 60), respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of moexipril and hydrochlorthiazide in tablets.

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