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Bhaskara Raju V.,Sri Vasavi Institute of Pharmaceutical science | Lakshmana Rao A.,VV Institute of Pharmaceutical science
Rasayan Journal of Chemistry | Year: 2011

An accurate and precise RP-HPLC method was developed for the determination of perindopril in tablet dosage forms. Separation of die drug was achieved on a reverse phase Cig column using a mobile phase consisting of phosphate buffer and acetonitrile in the ratio of 65:35 v/v. The flow rate was 0.6 ml/min and die detection wavelength was 209 nm. The linearity was observed in the range of 20-100 ug/ml with a correlation coefficient of 0.9997. The proposed method was validated for its linearity, accuracy, precision and robustness. This method can be employed for routine quality control analysis of perindopril in tablet dosage forms. © 2011 RASAYAN.


Sahoo P.K.,Sri Vasavi Institute of Pharmaceutical science | Behera P.,JKK Nataraja Dental College and Hospital
European Journal of Medicinal Chemistry | Year: 2010

The acetic acid derivatives of [1,2,4]triazino[4,3-a]benzimidazole (TBI) were synthesized and tested in vitro and in vivo as selective aldose reductase (ALR2) inhibitors. Compound PS11 showed highest inhibitory activity (IC50) 0.32 μM and was found to be effective in preventing cataract development in severely galactosemic rats when administered as an eyedrop solution. All the compounds investigated were selective for ALR2, since none of them inhibited appreciably aldehyde reductase, sorbitol dehydrogenase, or glutathione reductase. © 2009 Elsevier Masson SAS. All rights reserved.


Sahoo P.K.,Sri Vasavi Institute of Pharmaceutical science | Behera P.,JKK Nataraja Dental College and Hospital
European Journal of Medicinal Chemistry | Year: 2010

A series of novel ketoprofen derivatives 4a-j bearing both amide and carbamate functionalities were prepared using benzotriazole. Selective reduction of ketoprofen produced hydroxy derivative 2, which reacts with one or 2 mol of 1-benzotriazole carboxylic acid chloride (1) gave benzotriazole derivatives 3a and 3b respectively. Antioxidative screenings revealed that the prepared compounds 3b and 4a-j possess excellent lipid peroxidation inhibition at 0.1 mM concentration. Two of the compounds 3b and 4g also showed high soybean lipoxygenase inhibition activity, where as the amidocarbamate derivatives of ketoprofen showed only weak reducing activity against 1,1-diphenyl-2- picrylhydrazyl radicals. No selective antiviral effects were noted for the tested compounds against a broad variety of DNA and RNA viruses. © 2010 Elsevier Masson SAS.


Raju V.B.,Sri Vasavi Institute of Pharmaceutical science | Rao A.L.,VV Institute of Pharmaceutical science
E-Journal of Chemistry | Year: 2012

An accurate and precise HPLC method was developed for the determination of lisinopril. Separation of the drug was achieved on a reverse phase C 8 column using a mobile phase consisting of phosphate buffer and methanol in the ratio of 35:65v/v. The flow rate was 0.8 mL/min and the detection wavelength was 215 nm. The linearity was observed in the range of 20-60 μg/mL with a correlation coefficient of 0.9992. The proposed method was validated for its linearity, accuracy, precision and robustness. This method can be employed for routine quality control analysis of lisinopril in tablet dosage forms.


Thangavel N.,Sri Vasavi Institute of Pharmaceutical science | Gupta J.K.,Jadavpur University
E-Journal of Chemistry | Year: 2010

The antioxidant potency of various extracts of Andrographis stenophylla leaf was evaluated in vitro using ferric thiocyanate method. Reductive ability and free radical scavenging activity of the extracts were also investigated. Amounts of phenolic compounds in each of the extracts were determined using Folin-Ciocalteau reagent and compared to observe the correlation between antioxidant activities and total phenolic content. Methanol extract exhibited maximum antioxidant activity and was found to contain 2% of total phenolic compounds. Methanol extract was subjected to column chromatographic separation over silica gel G using ethyl acetate: formic acid: acetic acid: water. Fractions thus obtained were screened for their antioxidant activity. Among the eleven fractions screened, fraction C was more active than the standard butylated hydroxyanisole. Fraction C on further fractionation with n-butanol: acetic acid: water afforded two flavanoids namely acacetine and isosakuranetine. Fraction A was also shown to possess good antioxidant activity which was developed using TLC and indicated the presence of a terpenoid, Andrographolide. The structures of the isolated compounds were confirmed by UV, IR, MS, 1H and 13C NMR spectral data. This is the first report wherein Andrographolide, Acacetine and Isosakuranetine are isolated from Andrographis stenophylla leaf.

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