Charan Gowda B.K.,Sri Siddhartha Academy of Higher Education |
Kokila G.,Sri Siddhartha Academy of Higher Education |
Gopinathan P.A.,Sri Sankara Dental College |
Praveen K.S.,Sri Siddhartha Academy of Higher Education
Journal of Clinical and Diagnostic Research | Year: 2017
Introduction: Forensic dentistry is a branch of dentistry which in collaboration with legal profession serves an important role to maintain justice system of a country. Forensic dentists play a major role in identification of an individual. Within the literature various methods have been found to be useful in gender differentiation. An attempt was made for differentiation of gender using picrosirius red and polarization microscopy. Aim: To evaluate picrosirius red and polarization microscopy as a tool for gender differentiation by observing birefringence pattern and distribution of thick and thin collagen fibers in males and females. Materials and Methods: Labial mucosal tissue obtained from 30 deceased individuals (18 male and 12 female) during autopsy was fixed in 10% formalin at 12th hour. Tissue was processed, sectioned and stained using picrosirius red stain and the birefringence pattern of collagen fibers were studied with polarization microscope. The results were statistically analyzed using Z-test and one-way ANOVA to draw the significance. Results: The proportion of thick and thin fibres among males and females were compared. It was found that there was statistically significant difference in proportion of thick and thin fibers between male and female. Thick fibres in males were (78.13%) more than females (65.74%) and thin fibres were more in females (34.24%) than males (21.32%). Conclusion: Picrosirius red and polarization microscopy may be used as a tool for gender differentiation. Yet the manner of death has to be considered during gender differentiation using this method, as in the present study out of 30 cases studied three cases of death due to debilitating diseases and poison consumption showed altered collagen distribution. © 2017, Journal of Clinical and Diagnostic Research. All rights reserved.
Krishnan V.,Sri Sankara Dental College |
Bhatia A.,Sree Chitra Tirunal Institute for Medical Science and Technology |
Varma H.,Sree Chitra Tirunal Institute for Medical Science and Technology
Dental Materials | Year: 2016
Objectives: Enamel damage resulting or arising from/associated with orthodontic treatment such as white spot lesions and surface deterioration after debonding brackets along with incipient carious lesions are considered problems not amenable for routine restorations due to its invasive nature. The present study was aimed at synthesizing and characterizing nHAp and 25 and 50. mol% strontium nHAp as a surface application modality for dental enamel remineralization/repair. Methods: 25 and 50. mol% Sr nHAp was synthesized and characterized in comparison with custom made pure nHAp initially with the help of transmission and scanning electron microscopy as well as toxicological assessment. Further, comparative evaluation of these novel synthesized strontium substituted particles was assessed for its efficacy in repairing damaged enamel with the help of atomic force microscopy, scanning electron microscopy and micro indentation testing. Results: There is increase in crystallinity and reduced particle size favoring dissolution and re-precipitation through small incipient carious lesions and soft white spot areas with 25% Sr-nHAp. Sr doped specimens showed more cell viability in comparison with pure nHAP make it less cytotoxic and hence a biologically friendly material which can be safely applied in patient's mouth. AFM images obtained from 25% and 50% Sr nHAp treated specimens clearly indicated increased roughness in surface topography and performed well with micro indentation test. Significance: The novel synthesized Sr doped nHAp forms an improved treatment modality to tackle the long standing quest for solving the problem of enamel loss with incipient carious lesions and WSL from orthodontic procedures. © 2016 Academy of Dental Materials.
Jodalli P.S.,Yenepoya Dental College and Hospital |
Ankola A.V.,KLE University |
Hebbal M.,KLE University |
Vikneshan M.,Sri Sankara Dental College
Community Dental Health | Year: 2013
Objectives: To assess the perceptions of and concerns regarding dental fluorosis among 12-15 year-old schoolchildren in an area where fluorosis is endemic and to find any association with Tooth Surface Index of Fluorosis (TSIF) scores. Basic Research Design: A cross sectional study. Participants: 316 children aged 12-15 years with varying level of dental fluorosis selected from 696 children screened for fluorosis. Methods: The study was undertaken in three villages in rural Gadag, Karnataka, India, with fluoride levels in the drinking water ranging from 2.2 to 4.5 ppm as estimated by the SPADNS spectrophotometric method. Children's fluorosis was assessed under natural lighting conditions on the labial surface of upper anterior teeth using the TSIF 8-point scale. A self-administered questionnaire assessed knowledge, perceptions and concerns. Data were analysed by frequency distributions and Spearman's correlation coefficients. Main outcome measures: Aesthetic perceptions and TSIF scores. Results: The children were aware of the fluoride presence and its health effects. TSIF scores correlated with: the appearance of front teeth (r=0.135, p=0.015); feeling fluorosis would hinder smiling (r=0.116, p=0.040); feeling distress due to fluorosis (r=0.111, p=0.048). Conclusion: Children were aware of fluoride and its health effects. They also perceived dental fluorosis as an aesthetic problem. © BASCD 2013.
PubMed | ECHS Polyclinic, MR Ambedkar Dental College, Government Dental College and Research Institute, Guru Gobind Singh Institute of Dental science and Sri Sankara Dental College
Type: Journal Article | Journal: Indian journal of dental research : official publication of Indian Society for Dental Research | Year: 2016
Oral squamous cell carcinoma (OSCC) is the 8 th most common aggressive epithelial malignant neoplasm worldwide today. The eosinophil may be a multifaceted cell that can be associated with wound-healing processes, as well as to tissue damage which has increased the speculations around tumor-associated tissue eosinophilia in malignant tumors. The aim of this study was to detect the role and quantitative analysis of tumor-associated tissue eosinophils in different histological grades of OSCC.A retrospective study was carried out in sixty cases of histopathologically graded OSCCs. Tissue sections of 4 thickness were made from paraffin-embedded tissue blocks and were stained with hematoxylin and eosin. Eosinophils were counted under randomly selected twenty high-power (40) fields. Data were subjected to statistical analysis using ANOVA test.Higher mean eosinophils were recorded in well-differentiated squamous cell carcinoma (WDSCC) followed by moderately differentiated squamous cell carcinoma (MDSCC) and poorly differentiated squamous cell carcinoma (PDSCC) groups, respectively. The difference in mean eosinophils was found to be statistically significant between WDSCC and MDSCC (P < 0.001), as well as between WDSCC and PDSCC (P < 0.001).Tumor-associated tissue eosinophil count is higher in WDSCC as compared to moderate and PDSCC.
PubMed | Sri Sankara Dental College
Type: Journal Article | Journal: Journal of clinical and diagnostic research : JCDR | Year: 2015
Capillary Haemangioma is a benign vascular tumour characterized by proliferation of blood vessels that are primarily reported to be a developmental hamartomatous lesion of infancy and childhood. Pyogenic granuloma is a non-neoplastic benign lesion found in the oral cavity having a striking predilection for occurrence in the gingiva. The present case report is an atypical presentation of capillary haemangioma on gingiva which is considered to be extremely rare. The lesion in this case was clinically diagnosed as pyogenic granuloma but histopathologically as capillary haemangioma. These lesions present as a diagnostic dilemma to the clinician and can lead to serious complications if not carefully managed.
PubMed | Vishnu Dental College, Sri Sankara Dental College and Reader
Type: Journal Article | Journal: Journal of clinical and diagnostic research : JCDR | Year: 2016
Amputation of all or part of a limb may be due to systemic disease, vascular disease, infection, local injury or trauma. Partially amputated lower limbs present a variety of unique clinical and prosthetic challenges, because of distinctly different amputation levels of the lower limb. A female patient with history of Partial Foot Amputation (PFA) surgery at metatarsophalangeal joint level, due to crush injury reported for prosthetic rehabilitation. This case was successfully rehabilitated using room temperature vulcanizing (RTV) medical grade silicone for fabrication of foot prosthesis. Though limited in its function, it amply proves that the clinical and laboratory techniques used in fabrication of maxillofacial prosthesis can be effectively adapted for the fabrication of body prosthesis like toes and feet to enhance the quality of life of the patients. The patient was reviewed every year for three years.
PubMed | Salem College, Hail University and Sri Sankara Dental College
Type: Journal Article | Journal: Journal of cancer research and therapeutics | Year: 2016
Sialic acids are important terminal sugars of the carbohydrate chains of cell membrane glycoproteins and glycolipids. The progressive rise in total sialic acid (TSA) levels has been significantly associated with tumor progression and metastasis.Samples of 2 ml of unstimulated whole saliva were collected from 20 healthy patients, and 20 cases of histopathologically confirmed oral pre-cancer patients and 20 cases of histopathologically confirmed oral squamous cell carcinoma (OSCC) patients, using the method of Navazesh. Estimation of total sialic acid was done by the histochemical method of Yao et al.The study revealed significantly elevated total sialic acid (TSA) levels in the saliva of oral precancer and OSCC patients, yielding a mean value of 59.75 7.29 mg/dl and 204.85 60.38 mg/dl, respectively, against the mean value of 21.65 5.71 mg/dl of the healthy controls. Also, high total sialic acid (TSA) levels in the well-differentiated squamous cell carcinoma cases were appreciable to those of moderately differentiated ones.The result suggests the correlation of elevated salivary TSA levels with the progression of OSCC. In conclusion, the study indicates that the glycoprotein metabolism is significantly altered in the saliva of patients, with both oral pre-cancer and OSCC.
PubMed | Sri Sankara Dental College and Regional Cancer Center
Type: | Journal: Journal of addiction | Year: 2016
Objectives. Limited information is available on adolescent tobacco and alcohol use in rural Kerala, the southernmost state in India. The study was conducted to estimate the prevalence of tobacco and alcohol use among adolescent school students and further to understand the extent of knowledge pertaining to tobacco before and after conducting awareness programmes in schools. Methods. A cross-sectional study was conducted in 10 government schools of rural Thiruvananthapuram district of Kerala state based on a multistaged sampling design. Using a pretested semistructured questionnaire, prevalence and patterns of tobacco use by students and their households, as well as students knowledge on tobacco hazards before and after delivering antitobacco messages, were collected. Results. The overall prevalence of self-reported ever users of tobacco in the current academic year was 7.4% (95% CI 5.86-8.94), while that of ever alcohol users was 5.6% (95% CI 4.25-6.95). Knowledge assessment scores revealed a significant increase in the mean knowledge scores after posttraining evaluation (mean score = 10.34) when compared to pretraining evaluation (mean score = 9.26) (p < 0.0001). Conclusion. Apart from antitobacco awareness programmes, strict monitoring of trade of tobacco and alcohol products near educational institutions has to be conducted consistently to curb the problem.
PubMed | Maharashtra Institute of Dental science and Research, Prince Sattam bin Abdul Aziz University, Sri Sankara Dental College and Coorg Institute of Dental science
Type: Journal Article | Journal: Kathmandu University medical journal (KUMJ) | Year: 2016
Background Dental wastes are materials that have been utilized in dental clinics, which are no longer wanted for use and therefore discarded. Improper disposal of these dental wastes can cause harm to the dentist, the people in immediate vicinity of the dentist. Objective The present study was conducted to assess the knowledge and practices regarding Dental waste management among private practitioners. Method The study population included 186 private practitioners in three districts of Karnataka (Coorg, Mysore, Hassan), south India. A pre-tested self-administered questionnaire was distributed to assess the knowledge and practices regarding dental waste management. Descriptive statistics was used to summarize the results. The data will be statistically analyzed using chi-square test, correlation. Result Out of 186 study subjects, 71(38%) were females and 115(62%) were males, Chisquare analysis showed highly significant association between qualification of the study participants and their knowledge, practice of dental waste management. A statistically significant (0.0001) correlation was found between the knowledge and practice scores. Conclusion There was a lacunae of knowledge regarding proper biomedical waste disposal among the participants. In order to fill this vacuum CDE (Continuing Dental Education) programs have to be conducted in pursuance to maintain health of the community.
PubMed | Dental Officer, Sri Sankara Dental College, M R Ambedkar Dental College, Geetanjali Dental and Research Institute and 2 more.
Type: Journal Article | Journal: Journal of clinical and diagnostic research : JCDR | Year: 2016
The imprint produced by the lip is termed as lip print and the name given to the examination of lip print is referred to as cheiloscopy.The present study was done to compare the efficacy of three different materials used in cheiloscopy - Sudan III, Indigo and Aluminium powder.A total of 60 individuals belonging to the age group of 18-25 years were included in this study. Visible and latent lip prints were reproduced on white cotton fabric, white satin fabric and white clay cup. The results were statistically analysed using chi square test.Sudan III dye and Aluminium powder showed significant results when used for the development of visible lip prints (p<0.05), but didnt give any significant results for development of latent lip prints. Indigo dye gave significant results for development of both visible and latent lip prints (p<0.05).The study reveals the usefulness of visible and latent lip print in individual identification.