Sri Sankara Arts and Science College

Kānchipuram, India

Sri Sankara Arts and Science College

Kānchipuram, India
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PubMed | Tata Memorial Center, Sri Sankara Arts and Science College, University of Alabama at Birmingham and University of Texas Health Science Center at Tyler
Type: Journal Article | Journal: Clinical cancer research : an official journal of the American Association for Cancer Research | Year: 2016

The goal of this study was to understand the role of altered mitochondrial function in breast cancer progression and determine the potential of the molecular alteration signature in developing exosome-based biomarkers.This study was designed to characterize the critical components regulating mitochondrial function in breast tumorigenesis. Experiments were conducted to assess the potential of these molecules for exosome-based biomarker development.We observed a remarkable reduction in spontaneous metastases through the interplay in mitochondria by SH3GL2, vesicular endocytosis-associated protein and MFN2, an important regulator of mitochondrial fusion. Following its overexpression in breast cancer cells, SH3GL2 translocated to mitochondria and induced the production of superoxide and release of cytochrome C from mitochondria to the cytoplasm. These molecular changes were accompanied by decreased lung and liver metastases and primary tumor growth. SH3GL2 depletion reversed the above phenotypic and associated molecular changes in nontumorigenic and tumorigenic breast epithelial cells. Loss of SH3GL2 and MFN2 expression was evident in primary human breast cancer tissues and their positive lymph nodes, which was associated with disease progression. SH3GL2 and MFN2 expression was detected in sera exosomes of normal healthy women, but barely detectable in the majority of the women with breast cancer exhibiting SH3GL2 and MFN2 loss in their primary tumors.This study identified a new mitochondria reprogramming pathway influencing breast cancer progression through SH3GL2 and MFN2. These proteins were frequently lost in breast cancer, which was traceable in the circulating exosomes. Clin Cancer Res; 22(13); 3348-60. 2016 AACR.


Sivakumar S.,Sri Sankara Arts and Science College | Sivakumar S.,University of Madras | Niranjali Devaraj S.,University of Madras
Journal of Diabetes and Metabolic Disorders | Year: 2014

Background: Osteopontin (Eta, secreted sialoprotein 1, opn) is secreted from different cell types including cancer cells. Three splice variant forms namely osteopontin-a, osteopontin-b and osteopontin-c have been identified. The main astonishing feature is that osteopontin-c is found to be elevated in almost all types of cancer cells. This was the vital point to consider it for sequence analysis and structure predictions which provide ample chances for prognostic, therapeutic and preventive cancer research.Methods: Osteopontin-c gene sequence was determined from Breast Cancer sample and was translated to protein sequence. It was then analyzed using various software and web tools for binding pockets, docking and druggability analysis. Due to the lack of homological templates, tertiary structure was predicted using ab-initio method server - I-TASSER and was evaluated after refinement using web tools. Refined structure was compared with known bone sialoprotein electron microscopic structure and docked with CD44 for binding analysis and binding pockets were identified for drug designing.Results: Signal sequence of about sixteen amino acid residues was identified using signal sequence prediction servers. Due to the absence of known structures of similar proteins, three dimensional structure of osteopontin-c was predicted using I-TASSER server. The predicted structure was refined with the help of SUMMA server and was validated using SAVES server. Molecular dynamic analysis was carried out using GROMACS software. The final model was built and was used for docking with CD44. Druggable pockets were identified using pocket energies.Conclusions: The tertiary structure of osteopontin-c was predicted successfully using the ab-initio method and the predictions showed that osteopontin-c is of fibrous nature comparable to firbronectin. Docking studies showed the significant similarities of QSAET motif in the interaction of CD44 and osteopontins between the normal and splice variant forms of osteopontins and binding pockets analyses revealed several pockets which paved the way to the identification of a druggable pocket. © 2014 Sivakumar and Niranjali Devaraj; licensee BioMed Central Ltd.


Koodalingam A.,University of Madras | Koodalingam A.,Sri Sankara Arts and Science College | Mullainadhan P.,University of Madras | Arumugam M.,University of Madras
Acta Tropica | Year: 2013

We recently reported the presence of potent anti-mosquito activity in aqueous kernel extract of the soapnut, Sapindus emarginatus, and demonstrated its impact on marker enzymes in larvae and pupae of the vector mosquito, Aedes aegypti. As a sequel to these findings, the present study elucidates immunotoxicity of this extract with respect to hemocyte-mediated cellular immune responses in fourth instar larvae and pupae as well as cuticular melanization reaction in the larvae of A. aegypti. The exposure of these two developmental stages of the mosquito to the soapnut extract at a lethal threshold concentration neither affected hemocyte viability tested up to 3. h in vitro nor did it influence the hemocyte count. By contrast, exposure of the mosquito larvae and pupae to this extract significantly reduced the ability of their hemocytes to bind yeast cells, an important early event in the process of non-self recognition by immune cells. Consequently, the phagocytic activity of these hemocytes against yeast cells was also found to be adversely affected upon exposure of larvae and pupae to the extract. Besides, a perceptible initial delay in melanization reaction at the injured site of the cuticle in the extract-exposed larvae was observed. All these findings demonstrate, for the first time, the immuno-suppressive potential of a botanical biocide in the vector mosquito. © 2013 Elsevier B.V.


PubMed | SRM University, King Saud University, Tamil University, P.A. College and 2 more.
Type: | Journal: Fish & shellfish immunology | Year: 2016

The effect of diet supplemented with Shilajit, a multi-component natural mineral substance on the antioxidant activity, immune response, and disease resistance in freshwater prawn, Macrobrachium rosenbergii (de Man) against Aeromonas hydrophila is reported. The total hemocyte count (THC) and phagocytic activity significantly increased with 2gkg(-1) supplemented diet on first week and with other enriched diets on weeks 2 and 4. The respiratory burst (RB) activity and glutathione peroxidase (GPx) activity were significantly increased with 2gkg(-1) supplemented diet on weeks 1 and 2 whereas 2 and 4gkg(-1) diets on week 4. The phenoloxidase (PO) activity increased significantly with 2gkg(-1) diet only on second week and with other enriched diets only on fourth week. The superoxide dismutase (SOD) activity increased significantly with any enriched diet during the experimental period except with 6gkg(-1) diets on first week. However, the glutathione reductase (GR) activity was enhanced significantly only with 2gkg(-1) enriched diets on weeks 2 and 4. The cumulative mortality of the prawn fed with 2 and 4gkg(-1) enriched diets was 10% and 15% whereas with 6gkg(-1) diet the mortality was 20%. The results suggest that diet enriched with Shilajit at 2gkg(-1) or 4gkg(-1) positively enhances the antioxidant activity, immunity, and disease resistance in M.rosenbergii against A.hydrophila.


Gayathri S.,Sri Sankara Arts and Science College | Saravanan D.,Sri Sankara Arts and Science College | Radhakrishnan M.,Sri Sankara Arts and Science College | Balagurunathan R.,Periyar University | Kathiresan K.,Annamalai University
Indian Journal of Biotechnology | Year: 2010

In the present study, bioprospecting aspects of endophytic bacteria, isolated from leaves of 5 mangroves and 2 salt-marsh plant species, were explored. In total, 104 bacterial isolates were isolated from the leaf samples of mangrove plants of Pichavaram, Tamil Nadu. Thirty six fast growing isolates were selected and screened for biological activities. Of 36 isolates, 28 (77%) showed antimicrobial activity, and 94.4% of isolates exhibited pectinase, 58.3% protease and 52.7% inulinase and invertase activities. Plant growth promoting activity, such as, ammonia and acetoin production, was shown by 22 (61.1%) and 25 (69.4%) endophyte isolates, respectively; whereas 26 isolates (72.2%) showed nitrogen fixing activity. Six isolates (16.6%) showed phosphate solubilization activity and 7 isolates (19.4%) produced indole acetic acid (IAA). Further, malachite green and phenol degrading activities were observed in 12 (33.3%) and 20 (55.5%) endophytic bacterial isolates, respectively. More than 20 endophytic bacteria were sensitive to antibiotics like streptomycin and trimethoprim, while 31 isolates (86.1%) were resistant to vancomycin and bacitracin. Thirty four (94.4%) and 31 (86.6%) endophytic isolates exhibited growth onto a medium containing upto 7.5 and 10% NaCl concentrations, respectively. The present study has proved that the mangroves are the sources of endophytic bacteria with bioprospecting potential, which deserves further studies.


Sarala V.,Sri Sankara Arts and Science College | Radhakrishnan M.,Sri Sankara Arts and Science College | Balagurunathan R.,Periyar University
International Journal of ChemTech Research | Year: 2011

Biofouling bacteria were isolated from fouling sample collected from boat and other marine structures around Parangipettai coastal area (lat.11° 29′N; long.79° 46′E) and its population was estimated as 8.15×106 CFU/gram. Based on the result of adherence study, five different biofouling bacterial isolates were selected, characterized and identified as Pseudomonas sp., Alteromonas sp., Bacillus sp., Staphylococcus sp., and Serratia sp. Crude bioactive metabolites from 15 plant samples (leaves) were collected and were extracted using methanol and tested against biofouling bacteria by disc diffusion method in which nine extracts showed inhibition against biofouling bacteria. Andrographis paniculata was selected for further isolation of antifouling compound, as it showed maximum of 13-21mm zone of inhibition. Of the various solvents tested for extraction methanol extract showed best activity followed by ethanol, ethyl acetate, n-hexane and aqueous extract. Antifouling compound was separated by TLC. The active compound separated in TLC was detected by bioautography in which the first spot (Rf value - 0.96) showed antifouling activity. Based on the results of phytochemical analysis the active compound was identified as terpenoids. The partially purified fraction was tested for biofouling inhibition and prevention by cover glass method, and wooden stick method. In both the studies the partially purified fraction showed good activity. Further investigation of active compound from A. paniculata will leads to the development of economically cheaper and ecofriendly antifouling compounds.


Saravanan D.,Sri Sankara Arts and Science College | Bharathi S.,Sri Sankara Arts and Science College | Radhakrishnan M.,Sri Sankara Arts and Science College | Balagurunathan R.,Periyar University
Journal of Applied Pharmaceutical Science | Year: 2012

This study deals with bacterial prospecting from forest soil with special reference to antimicrobial substances. Total of 25 morphologically different bacterial colonies were isolated from soil samples collected from Anaimalai forest and Parambikulam tiger reserve forest, Western Ghats, India. About 12 (48%) out of 25 isolates showed antibacterial activity in which strain AF1 showed inhibitory activity against more number of test pathogens. Bioactive substance from strain AF1 was produced by adopting submerged fermentation and extracted usingethyl acetate and chloroform. In disc diffusion method, ethyl acetateextract showed good antibacterial activity (9-17 mm zone of inhibition). Active fraction present in the ethyl acetate extract was determined by thin layer chromatography based bioautography. Findings of thiswork supported that the forest ecosystems investigated in this study will be potential place for bacterial bioprospecting.


Albino Wins J.,Holy Cross College | Murugan M.,Sri Sankara Arts and Science College
International Journal of Pharma and Bio Sciences | Year: 2010

The effect of textile effluent was studied with respect to germination and growth of black gram Vigna mungo (L.) Hepper. In lower concentration the germination ratio and growth are relatively higher than the control, but gradual decrease in the germination of seeds, seedling growth with increase in effluent concentration was observed. The best germination and seedling growth was observed in 25%concentration with growth promoting effect, and significantly better than control. Beyond 25% effluent, root and shoot length decreased. Thus the textile mill effluent can be safely used for irrigation purposes with proper treatment and dilution at 25%.


Koodalingam A.,Sri Sankara Arts and Science College | Deepalakshmi R.,Sri Sankara Arts and Science College | Ammu M.,Sri Sankara Arts and Science College | Rajalakshmi A.,Sri Sankara Arts and Science College
Journal of Asia-Pacific Entomology | Year: 2014

Many of the neem based botanical biocides are currently studied to a greater extent because of the possibility of their use in eco-friendly control of pests and vectors. However, no report was available to assess the impact of neem based formulation, NeemAzal on marker enzymes and hemocyte mediated cellular immune responses of important vector mosquito A. aegypti. The NeemAzal found to exert larvicidal and pupicidal activities against A. aegypti developmental stages. The pupae appear to be more susceptible to the treatment. Further, a significant increase in the level of total protein (31%), α-carboxylesterase (121%), β-carboxylesterase (46%), acid phosphatase (62%) and alkaline phosphatase (37%) was observed in larvae upon exposure to NeemAzal. Moreover, treated pupae showed increased level of acetylcholinesterase (116%) and acid phosphatase (43%) while α-carboxylesterase (34%), β-carboxylesterase (12%) levels were simultaneously decreased, and no significant changes in alkaline phosphatase were noticed. Qualitative analysis also revealed that the exposure considerably modulated the larval β-carboxylesterase isoenzyme profile whereas little changes were noticed on phosphatases. On the other hand hemocyte viability of larvae (18%) and pupae (16%) as well as phagocytic ability of larval (48%) and pupal hemocytes (44%) against yeast target was significantly reduced upon NeemAzal exposure. We demonstrated for the first time that the NeemAzal differentially affected the marker enzymes and created immuno-suppressive state by reducing the phagocytic ability of hemocytes of larvae and pupae of A. aegypti. © 2013 Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society.


Arunachalam C.,Sri Sankara Arts and Science College | Narmadhapriya D.,Sri Sankara Arts and Science College
Asian Journal of Pharmaceutical and Clinical Research | Year: 2011

Monascus fermented rice has been traditionally used as a natural food colorant and food preservative of meat and fish for centuries. Monascus fermented products gradually regarded as the functional food because the monacolin K (antihypercholesterolemic agents), γ-aminobutyric acid (GABA) (hypotensive agent), and dimerumic acid (antioxidant) were found. This review article describes about the History and traditional use of Monascus fermented rice, Fermentation conditions for pigment production and Pharmacological aspects which include effects in cholesterol lowering, Anti-Diabetic Activity, Anti-Inflammation and prevention of Osteoporosis.

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