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Lakshmi G.K.,Vvvanniaperumal College For Women | Krishnaveni K.,Sri Ramasamy Naidu Memorial College
Proceedings - 2014 World Congress on Computing and Communication Technologies, WCCCT 2014

In this paper, methods for automated extraction of multiple features of cytoplasm and nuclei from cervical cytology images are described. Edges of the image are enhanced by Edge Sharpening filter. Then Gaussian mixture model using Expectation Maximization and K-means clustering is used to segment the image into its components as background, nucleus and cytoplasm. Features have been identified for both multiple and single cervical cytology cells. For multiple cell images, nucleus to cytoplasm ratio is calculated. A mixture of features like center, perimeter, area, mean intensity of nucleus and cytoplasm are extracted from cells with single nucleus. These features may be used to determine the stage of cancer. © 2014 IEEE. Source

Kavitha T.,Sri Ramasamy Naidu Memorial College | Kulandaisamy A.,Raja Doraisingam Government Arts College | Thillaiarasu P.,Kalasalingam University
International Journal of ChemTech Research

Neutral tetradentate N 2O 2 complexes of Cu(II), Ni(II), Co(II), Zn(II) and VO(II) have been synthesized using the 1-phenyl-2,3-dimethyl-4-imino-(2-hydroxybenzylidene)-pyrozol-5-(α-imino)-indole- 3-propionic acid (H 2L). All the complexes were characterized by elemental analysis, molar conductivity, magnetic susceptibility data, IR, 1H-NMR, UV-Vis, FAB-Mass, and EPR spectral studies. The physicochemical studies and spectral data indicate that the ligand acts as a divalent tetradentate chelating agent. All the complexes have the general composition [ML)] (M= Cu(II), Ni(II), Co(II), VO(II) and Zn(II); L = Schiff base). The IR, UV-Vis., magnetic susceptibility measurements and EPR spectral data of the complexes suggest that all the complexes are square planar geometry around the central metal ion except VO(II) complex which has square pyramidal geometry. The lower conductivity data confirm the non-electrolytic nature of the complexes. The effect of redox potential on metal ion by the ligand environment is studied by cyclic voltametric measurements. The Schiff base and its metal complexes were utilized to test the in vitro antimicrobial activities, which gave good results in the presence of metal ion than the free ligand environment against the different species of microorganisms. Source

Gurusamy P.,Sri Ramasamy Naidu Memorial College | Muthukumar K.,Sri Ramasamy Naidu Memorial College | Muthukumar K.,Goethe University Frankfurt | Rajesh S.,Biomedical Research Laboratory | And 3 more authors.
Journal of Structural Biology

Dopamine (3,4-dihydroxyphenylethylamine, DA), an important neurotransmitter, exists in the cell bodies of the dopaminergic neurons of the substantia nigra. Oxidation of DA to its quinone and subsequent reaction with Adenine and Guanine in DNA result in the formation of depurinating adducts, thus causing DNA damage. In this article, we investigate the interaction of quinone metabolites of dopamine (DMQ) with models representing the structure of DNA using dispersion corrected density functional theory with an aim to evaluate the associated structural changes in DNA upon their interaction. Various binding sites for the DA metabolite on these DNA models have been considered and our computations on the activation barriers allowed us to identify preferential bonding sites for these metabolites analogous to experiments. Analysis of the geometry of these adducts in comparison to free base pairs reveals that the attack of DMQ causes remarkable changes in the structural properties. With our calculations, we propose that these structural alterations induce mutations by favoring the formation of depurinating adducts leading to mutagenic effects such as base mispairing, explaining the toxicological (carcinogenic and neurotoxic) behavior of DMQ. © 2012 Elsevier Inc. Source

Muthukumar K.,Sri Ramasamy Naidu Memorial College | Muthukumar K.,University of Cincinnati | Gurusamy P.,Sri Ramasamy Naidu Memorial College | Gurusamy P.,Biomedical Research Laboratory | And 2 more authors.
Computational and Theoretical Chemistry

Acrylamide (AA), a potent carcinogenic material to animals, is reported to be present in a wide range of human consumed foods at the levels of several lg/kg. Glycidamide (GA), a metabolic product of AA formed via epoxidation, has been found to be responsible for the carcinogenicity. In order to resolve the carcinogenicity of acrylamide to humans, the yet unknown mechanistic features of GA attack on the base pairs have to be understood. Hence, in this study we investigated the possible mechanism by which the reactions between (GA) and various models representing DNA occur. Our investigation by computing the activation barriers and analyzing thermodynamic parameters for the attack of GA on three different sites (N 3, N 7 and O) of guanine in the G:C pair indicated that N 7 is the preferred position in agreement with the experimental data. Further geometrical optimization studies on the GA/AA adducts with G:C pair revealed that only the attack of GA on G:C pair caused remarkable structural alterations in DNA viz., major and minor groves perhaps leading to its carcinogenic behavior. © 2010 Elsevier B.V. Source

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