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Saroja N.,P.A. College | Laxminarayana E.,Sreenidhi Institute of Science and Technology Autonomous | Prasad K.R.S.,Koneru Lakshmaiah College of Engineering | Shanmukha Kumar J.V.,Koneru Lakshmaiah College of Engineering
Der Pharma Chemica | Year: 2012

2-Bromo-7-formyl-9,9-diethylfluorene and 2-aminothiophenol/2-aminophenol were condensed to form 2-(7- Bromo-9,9-diethyl-2-fluorenyl)benzothiazole/benzoxazole (2a-b). 2-(7-bromo-9,9-diethyl-2-fluorenyl)benzothiazole/benzoxazole reacted with 3-hydroxydiphenylamine, in presence of Pd(dba) 0 and diphenylphosphinoferrocene to form 2-(7-(3-benzyloxydiphenylamino)-9,9-diethyl-2-fluorenyl))benzothiazole/benzoxazole (3a-b) then the compound was debenzylated to give 2-(7-(3-hydroxydiphenylamino)-9,9-diethyl-2-fluorenyl)benzothiazole/benzoxazole (4a-b).


Sanaboina C.,GVK Biosciences Private Ltd | Sanaboina C.,Sreenidhi Institute of Science and Technology Autonomous | Jana S.,GVK Biosciences Private Ltd | Chidara S.,GVK Biosciences Private Ltd | And 3 more authors.
Tetrahedron Letters | Year: 2012

Synthesis of indole alkaloid related compounds using Schiff base formation, intramolecular cyclization (or N-alkylation), and Pictet-Spengler reaction as a cascade one pot condensation has been reported. The cascade chemistry has been applied to the synthesis of (±)-harmicine as a key step. © 2012 Elsevier Ltd. All rights reserved.


Vutukuru P.,Mediciti Institute of Medical science | Vutukuru S.S.,Sreenidhi Institute of Science and Technology Autonomous
Asian Journal of Microbiology, Biotechnology and Environmental Sciences | Year: 2015

Current aquaculture practices potentially lead to increased levels of antibiotic residues and antibiotic-resistant bacteria both in aquaculture products and their immediate environment. Research is warranted on the environmental and health impact of the use of antibiotics in aquaculture. In this study, the toxic impact of doxycycline on the glycogen and protein content of D. rerio exposed to 5, 10, 20 and 40 mg/L for 24 and 96 h was investigated. Loss of scales, hyper secretion of mucus, loss of balance, irregular swimming patterns were noticed in the fish exposed to 10 mg/L, 20 mg/L and 40 mg/L. Significant decline in the glycogen was observed in the gill and muscle tissues in 20 and 40 mg/L at 24 h while this decrease was observed even at 10 mg/L at 96 h. However, the decrease in glycogen of viscera exposed to all concentrations was insignificant at 24 and 96 h. After 24 h, the total protein content in gill decreased by 17.53%, 30.89%, 48.36% and 66.56%, and by 25.50%, 38.14%, 53.99% and 75.75% from control at 96 h, in 5, 10, 20 and 40 mg/L, respectively. Similar trends were also observed in muscle and viscera. Insignificant decrease was observed in protein content in gill, muscle and viscera of fish exposed to 5 mg/L at 24 h and 96 h. Zebra fish can be effectively used as model for understanding drug toxicity. The drug induced significant changes in glycogen and protein content of gills, muscle, viscera of the fish demonstrating a time concentration relationship. The occurrence of doxycycline at >10 mg/L in aquatic ecosystems is a matter of great concern in public health perspective via transmittance of antibiotic resistant genes. © 2015 Global Science Publications.


Laxminarayana E.,Sreenidhi Institute of Science and Technology Autonomous | Karunakar T.,Sreenidhi Institute of Science and Technology Autonomous | Ravi Kumar B.,Sreenidhi Institute of Science and Technology Autonomous | Venkat Reddy P.,Sreenidhi Institute of Science and Technology Autonomous
Der Pharma Chemica | Year: 2012

The target molecules 1,2-dihydro-2,3-dimethyl-1-phenyl-4H-pyrazolo[4,3-e][1,2,4]triazin-5(6H) -one and 4-(2-(3,5- dimethyl-1H-pyrazol-4-yl)diazenyl)-1,2-dihydro-1,5-dimethyl-2-phenylpyra zol-3-one were synthesized by eco friendly microwave irradiation. The chemistry of the reactions employed in the synthesis of the target compounds together with their chemical behavior, are discussed and the structures of the newly synthesized compounds were confirmed by the IR and 1H-NMR and Mass spectral data.


Sajjalaguddam R.R.,Sreenidhi Institute of Science and Technology Autonomous | Paladugu A.,Sreenidhi Institute of Science and Technology Autonomous
Journal of Applied Pharmaceutical Science | Year: 2015

Flavonoids have aroused considerable interest because of their potential beneficial effect on human health. Quercetin is one of the most abundant natural flavonoids present in medicinal plants. In the present study, a comparative phytochemical analysis of bioactive compounds from leaf and leaf derived callus of Abutilon indicum L. was carried out. Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) analysis of methanolic extract of Abutilon leaf revealed the presence of quercetin. To test the effect of precursor in enhancing flavonoid content, in vitro studies were conducted by supplementing phenylalanine to actively growing callus cultures of Abutilon. More than threefold increase in quercetin content was obtained in elicitor induced callus compared to control. © 2015 Ramgopal Rao Sajjalaguddam and Anitha Paladugu.


Vutukuru S.S.,Sreenidhi Institute of Science and Technology Autonomous | Ganugapati J.,Sreenidhi Institute of Science and Technology Autonomous | Ganesh V.,Sreenidhi Institute of Science and Technology Autonomous | Atheeksha P.,Sreenidhi Institute of Science and Technology Autonomous | Potti R.B.,Sreenidhi Institute of Science and Technology Autonomous
Fish Physiology and Biochemistry | Year: 2016

Endocrine disrupting chemicals may induce adverse health effects in humans and wildlife. Recent studies demonstrate that endocrine disrupting chemicals like Bisphenol A (BPA), polychlorinated biphenyls (PCBs) and polybrominated diphenyl ether (PBDE) affect the reproductive characters shared by wide range of creatures including fish. An attempt was made to evaluate the toxicity of these chemicals on the vitellogenin protein of zebra fish (Danio rerio) using in silico approach. The protein structure of zebra fish vitellogenin was predicted using homology modelling, and the stereochemical quality of the model was validated by Ramachandran plot. The 3-D structure of vitellogenin was docked with the aforementioned chemicals that have demonstrated endocrine-disrupting activity. The pair-wise alignments between vitellogenin with phosvitin, lipovitellin-2 and YGP40 obtained by CLUSTALW alignment suggest that the vitellogenin contained lipovitellin-2- phosvitin- and YGP40-related amino acid sequences. Based on the prediction of CASTp and CLUSTALW, BPA and PCB predominantly interacted with lipovitellin-2 site of the protein, while PBDE interacts predominantly with the YGP40 site of the vitellogenin protein. The results indicate that the endocrine-disrupting chemicals (BPA, PCB and PBDE) dock with the vitellogenin cleavage sites lipovitellin-2 and YGP40 that play a crucial role in lipid–protein complex formation in the egg yolk. We hypothesize that these chemicals could potentially impair the egg yolk formation and eventually impact the zebra fish population which occupies an important niche among testing models used in drug discovery and related toxicity studies. © 2016 Springer Science+Business Media Dordrecht


Paramesh D.,Sreenidhi Institute of Science and Technology Autonomous | Kumar K.V.,Jawaharlal Nehru Technological University | Reddy P.V.,Sreenidhi Institute of Science and Technology Autonomous
Processing and Application of Ceramics | Year: 2016

Ni-Zn-Al mixed ferrite nanoparticles, with general formula NixZn1-xAlFeO4 (x = 0.0, 0.2, 0.4, 0.6, 0.8 and 1.0), were synthesized by sol-gel auto combustion technique. All prepared ferrite nanoparticles were characterized by X-ray diffraction, scanning electron microscopy, energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy and vibrating sample magnetometer. X-ray diffraction study confirmed the formation of the single phase cubic spinel structure in all ferrite samples. The crystallite size was calculated by the Debye-Scherrer formula and found to be in the range 15-46 nm. The lattice constant decreased with increasing Ni2+ ion concentration. Scanning electron microscopy images clearly indicate that the particles are very small but agglomerated. Energy dispersive X-ray was used to confirm the composition of the prepared powders. Fourier transform infrared spectra showed two main absorption bands of ferrite nanoparticles, the high frequency band (v1) around 600 cm-1 and the low frequency band (v2) around 400 cm-1 arising from tetrahedral (A) and octahedral (B) interstitial sites in the spinel lattice, respectively. Vibrating sample magnetometer results reported that the saturation magnetization, remanent magnetization and magnetic moments decrease with increasing Ni+2 ion concentration.


Rao K.R.,Dr. Reddys Laboratories Ltd | Raghunadh A.,Dr. Reddys Laboratories Ltd | Mekala R.,Dr. Reddys Laboratories Ltd | Meruva S.B.,Dr. Reddys Laboratories Ltd | And 6 more authors.
Tetrahedron Letters | Year: 2014

A dual reactant/catalyst role of glyoxylic acid in the reaction of isatoic anhydride with various amines afforded a novel, robust and rapid synthesis of 3-(un)substituted quinazolin-4(3H)-ones. This metal catalyst-free reaction proceeds via an unusual and unexpected cleavage of C-C bond. A shorter and common route to two alkaloids, that is, rutaecarpine and evodiamine is also accomplished. © 2014 Elsevier Ltd. All rights reserved.


PubMed | Sreenidhi Institute of Science and Technology Autonomous
Type: Journal Article | Journal: Fish physiology and biochemistry | Year: 2016

Endocrine disrupting chemicals may induce adverse health effects in humans and wildlife. Recent studies demonstrate that endocrine disrupting chemicals like Bisphenol A (BPA), polychlorinated biphenyls (PCBs) and polybrominated diphenyl ether (PBDE) affect the reproductive characters shared by wide range of creatures including fish. An attempt was made to evaluate the toxicity of these chemicals on the vitellogenin protein of zebra fish (Danio rerio) using in silico approach. The protein structure of zebra fish vitellogenin was predicted using homology modelling, and the stereochemical quality of the model was validated by Ramachandran plot. The 3-D structure of vitellogenin was docked with the aforementioned chemicals that have demonstrated endocrine-disrupting activity. The pair-wise alignments between vitellogenin with phosvitin, lipovitellin-2 and YGP40 obtained by CLUSTALW alignment suggest that the vitellogenin contained lipovitellin-2- phosvitin- and YGP40-related amino acid sequences. Based on the prediction of CASTp and CLUSTALW, BPA and PCB predominantly interacted with lipovitellin-2 site of the protein, while PBDE interacts predominantly with the YGP40 site of the vitellogenin protein. The results indicate that the endocrine-disrupting chemicals (BPA, PCB and PBDE) dock with the vitellogenin cleavage sites lipovitellin-2 and YGP40 that play a crucial role in lipid-protein complex formation in the egg yolk. We hypothesize that these chemicals could potentially impair the egg yolk formation and eventually impact the zebra fish population which occupies an important niche among testing models used in drug discovery and related toxicity studies.


Vutukuru S.S.,Sreenidhi Institute of Science and Technology Autonomous | Raparthi S.,Sreenidhi Institute of Science and Technology Autonomous | Basani K.,Sreenidhi Institute of Science and Technology Autonomous
International Journal of ChemTech Research | Year: 2013

Titanium dioxide nanoparticles (nTiO2) are usually perceived as non-toxic, and have been extensively used in many products and applications. Whilst short-term studies that revealed no/low toxicity of nTiO2 to cells and eco-relevant organisms, the long-term impact of nTiO2.on the environment and human health is still a concern and warrants research. Keeping this in view, we made an attempt to examine the biochemical changes in Danio rerio exposed to 2.5, 5.0, 7.5, 10 and 12.5 mg/L of nTiO2. There were noticeable changes in the oxygen consumed by the fishes exposed to these concentrations which shows that the toxicant proved to be a potential respiratory inhibitor (p<0.01) in 12.5mg/L and 10.0mg/L at the end of 96h, (p<0.05) in 12.5mg/L and 10.0mg/L at the end of 24h, (p<0.05) in 7.5mg/L at the end of 96h respectively, demonstrating a timeconcentration relationship. Significant decrease was observed in total glycogen and total protein at the end of 96h in fish exposed to 10 and 12.5 mg/L.

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