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Gnanaprakasam Thankam F.,Sree ChitraTirunal Institute for Medical science and Technology | Muthu J.,Sree ChitraTirunal Institute for Medical science and Technology
Carbohydrate Polymers | Year: 2014

Alginate based hybrid copolymer hydrogels with unidirectional pore morphology were prepared to achieve synergistic biological performance for cardiac tissue engineering applications. Alginate based hybrid copolymer (ALGP) were prepared using alginate and poly(propylene fumarate) (HT-PPF) units. Different hybrid bimodal hydrogels were prepared by covalent crosslinking using poly(ethylene glycol diacrylate) and vinyl monomer viz acrylic acid, methyl methacrylate, butyl methacrylate and N-N′-methylene-bis-acrylamide and ionic crosslinking with calcium. The morphologically modified hydrogels (MM-hydrogels) with unidirectional elongated pores and high aspect ratio were prepared. MM-hydrogels favour better mechanical properties; it also enhances cell viability and infiltration due to unidirectional pores. However, the crosslinkers influence the fibroblast infiltration of these hydrogels. Synthesis of collagen and fibroblast infiltration was greater for alginate copolymer crosslinked with poly(ethylene glycol diacrylate-acrylic acid (ALGP-PA) even after one month (288%). This hybrid MM-hydrogel promoted cardiomyoblast growth on to their interstices signifying its potent applications in cardiac tissue engineering. © 2014 Published by Elsevier Ltd.


Deepa R.,Sree ChitraTirunal Institute for Medical science and Technology | Paul W.,Sree ChitraTirunal Institute for Medical science and Technology | Anilkumar T.V.,Sree ChitraTirunal Institute for Medical science and Technology | Sharma C.P.,Sree ChitraTirunal Institute for Medical science and Technology
Journal of Biomaterials and Tissue Engineering | Year: 2013

For fabricating skin grafts with differential healing properties, enrichment with biomolecules/cells may be desirable. Earlier, we have developed a biomaterial-quality chitosan for biomedical application. Here we evaluated the wound healing potential of the chitosan-preparation and its variant, prepared by loading homologus fibroblast. Dermal fibroblasts isolated from rabbit skin were seeded on chitosan sponge to fabricate a homologous fibroblast loaded chitosan graft (HFLC). Full thickness excision wounds created on the rabbit dorsum were grafted with these two types of the chitosan sponges; naked or non cellular chitosan graft (NCC) and the homologous fibroblast loaded chitosan graft (HFLC). Post-graft skin-wound samples were examined histomorphologically at 7th, 14th and 21st day for evaluating the nature of the tissue reaction induced by the grafts. The wound healing parameters considered were the extent of re-epithelialisation, collagen deposition and angiogenesis, the thickness of epidermis, number of proliferating cells, vimentin positive cells and alpha smooth muscle actin cells. The results suggested that both the grafts aided wound healing but the HFLC induced a differential pattern of healing at 7 and 14 days featured by enhanced angiogenesis, desmoplasia and a hyperkeratotic dermis. Under therapeutic conditions, the HFLC may be useful for regulating the extent of collagen deposition in the early phases of a healing wound. © 2013 American Scientific Publishers, All rights reserved.


Revi D.,Sree ChitraTirunal Institute for Medical science and Technology | Paul W.,Sree ChitraTirunal Institute for Medical science and Technology | Anilkumar T.V.,Sree ChitraTirunal Institute for Medical science and Technology | Sharma C.P.,Sree ChitraTirunal Institute for Medical science and Technology
Journal of Biomedical Materials Research - Part A | Year: 2014

This study evaluated the modulatory effect of chitosan sponge co-cultured with keratinocyte and fibroblast on wound healing. Dermal fibroblasts and keratinocyte isolated from rabbit skin were co-cultured on chitosan sponge, to fabricate cell-loaded chitosan tissue engineered construct. Full thickness excision wounds created on the rabbit dorsum were treated with three types of graft materials - a noncellular chitosan graft, homologous keratinocyte fibroblast loaded chitosan, and a commercial product. Postgraft skin-wound samples were examined histomorphologically at 7th, 14th, and 28th day after staining with hematoxylin and eosin, picrosirius red and/or immunohistochemistry. Wound healing parameters considered were the extent of re-epithelialization, collagen deposition, and neoangiogenesis. The number of proliferating cells, vimentin positive cells, and alpha smooth muscle actin cells were also quantified. The histology results suggested that the grafts aided wound healing but, the cell-loaded graft induced a differential pattern of healing and had lower scarring tendency. The cell-loaded tissue construct may be useful as a therapeutic graft for treating wounds where there is a total loss of tissue and cells as in burn injury. © 2013 Wiley Periodicals, Inc. J BiomedMater Res Part A: 102A: 3273-3281, 2014 © 2013 Wiley Periodicals, Inc.

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