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Moscow, Russia

Samatov T.R.,SRC Bioclinicum | Samatov T.R.,Moscow State University of Mechanical Engineering | Wicklein D.,University of Hamburg | Tonevitsky A.G.,Moscow State University
Progress in Histochemistry and Cytochemistry | Year: 2016

L1CAM is a cell adhesion molecule of the immunoglobulin superfamily which was originally discovered as a major player in the development of the nervous system. L1CAM was demonstrated to have prognostic value in different cancers and to be a promising target for anti-cancer therapy. Here we overview the present data on L1CAM structure and function, regulation of its expression, role in cancer and therapeutic potential. © 2016 Elsevier GmbH. Source


Marx U.,TU Berlin | Walles H.,University of Wurzburg | Hoffmann S.,TU Berlin | Lindner G.,TU Berlin | And 6 more authors.
ATLA Alternatives to Laboratory Animals | Year: 2012

Various factors, including the phylogenetic distance between laboratory animals and humans, the discrepancy between current in vitro systems and the human body, and the restrictions of in silico modelling, have generated the need for new solutions to the ever-increasing worldwide dilemma of substance testing. This review provides a historical sketch on the accentuation of this dilemma, and highlights fundamental limitations to the countermeasures taken so far. It describes the potential of recently-introduced microsystems to emulate human organs in 'organ-on-a-chip' devices. Finally, it focuses on an in-depth analysis of the first devices that aimed to mimic human systemic organ interactions in 'human-on-a-chip' systems. Their potential to replace acute systemic toxicity testing in animals, and their inability to provide alternatives to repeated dose long-term testing, are discussed. Inspired by the latest discoveries in human biology, tissue engineering and microsystems technology, this review proposes a paradigm shift to overcome the apparent challenges. A roadmap is outlined to create a new homeostatic level of biology in 'human-on-a-chip' systems in order to, in the long run, replace systemic repeated dose safety evaluation and disease modelling in animals. Source


Samatov T.R.,SRC Bioclinicum | Tonevitskaya S.A.,Moscow State University of Mechanical Engineering
Progress in histochemistry and cytochemistry | Year: 2015

The metastatic cascade comprises the following steps in sequential manner: the future metastatic cell has to leave the primary tumor mass, degrade the surrounding extracellular matrix, extravasate and circulate within in the bloodstream. Thereafter it has to attach to the endothelium of a target organ, intravasate into the connective tissue and has to proliferate to form a clinically detectable metastasis. We overview the in vitro microfluidic platforms modelling the metastatic cascade and the evolution towards systems capable of recapitulating all the steps by a single comprehensive model. Copyright © 2015. Published by Elsevier GmbH. Source


Davydov I.I.,SRC Bioclinicum | Wohlgemuth I.,Max Planck Institute for Biophysical Chemistry | Artamonova I.I.,Russian Academy of Sciences | Urlaub H.,Max Planck Institute for Biophysical Chemistry | And 3 more authors.
Nature Communications | Year: 2013

The emergence of ribosomes and translation factors is central for understanding the origin of life. Recruitment of translation factors to bacterial ribosomes is mediated by the L12 stalk composed of protein L10 and several copies of protein L12, the only multi-copy protein of the ribosome. Here we predict stoichiometries of L12 stalk for >1,200 bacteria, mitochondria and chloroplasts by a computational analysis, and validate the predictions by quantitative mass spectrometry. The majority of bacteria have L12 stalks allowing for binding of four or six copies of L12, largely independent of the taxonomic group or living conditions of the bacteria, whereas some cyanobacteria have eight copies. Mitochondrial and chloroplast ribosomes can accommodate six copies of L12. The last universal common ancestor probably had six molecules of L12 molecules bound to L10. Changes of the stalk composition provide a unique possibility to trace the evolution of protein components of the ribosome. © 2013 Macmillan Publishers Limited. All rights reserved. Source


Turchinovich A.,German Cancer Research Center | Turchinovich A.,University of Heidelberg | Samatov T.R.,SRC Bioclinicum | Tonevitsky A.G.,Russian Academy of Medical Sciences | And 2 more authors.
Frontiers in Genetics | Year: 2013

Nuclease resistant extracellular miRNAs have been found in all known biological fluids. The biological function of extracellular miRNAs remains questionable; however, strong evidence suggests that these miRNAs can be more than just byproducts of cellular activity. Some extracellular miRNA species might carry cell-cell signaling function during various physiological and pathological processes. In this review, we discuss the state-of-the-art in the field of intercellular miRNA transport and highlight current theories regarding the origin and the biological function of extracellular miRNAs. © 2013 Turchinovich, Samatov, Tonevitsky and Burwinkel. Source

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