SRA Intruments SpA

Cernusco sul Naviglio, Italy

SRA Intruments SpA

Cernusco sul Naviglio, Italy
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Magagna F.,University of Turin | Liberto E.,University of Turin | Reichenbach S.E.,University of Nebraska - Lincoln | Tao Q.,GC Image, LLC | And 5 more authors.
Journal of Chromatography A | Year: 2017

The possibility to transfer methods from thermal to differential-flow modulated comprehensive two-dimensional gas chromatographic (GC. ×. GC) platforms opens interesting perspectives for routine analysis of complex samples. Flow modulated platforms avoid the use of cryogenics, thereby simplifying laboratory operations and analyst supervision during intensive analytical sessions.This study evaluates the feasibility of transferring a fingerprinting method capable of classifying and discriminating cocoa samples based on the volatiles fraction composition according to their origin and processing steps. Previously developed principles of GC×GC method translation are applied to an original fingerprinting method, developed for a loop-type thermal modulated GC×GC-MS system, to engineer a method for a reverse-injection differential flow modulated platform (GC×2GC-MS/FID) with a dual-parallel secondary column and dual detection. Effective method translation preserves analytes elution order, 1D resolution, and 2D pattern coherence.The experimental results confirm the feasibility of translating fingerprinting method conditions while preserving the informative power of 2D peak patterns for sample classification and discrimination. Correct translation enables effective transfer of metadata (e.g., compound names and MS fragmentation patterns) by automatic template transformation and matching from the original/reference method to its translated counterpart. Although the adoption of a narrow bore (i.e. 0.1. mm dc ) column in the first-dimension enabled operation under close-to-optimal conditions with the differential-flow modulation platform, due to the dual-parallel columns in the second-dimension, it resulted in lower overall method sensitivity. Nevertheless, fingerprinting accuracy was preserved and most of the key-aroma compounds and technological markers were effectively mapped, thus limiting the loss of fingerprinting information. © 2017 Elsevier B.V.


Cordero C.,University of Turin | Rubiolo P.,University of Turin | Cobelli L.,SRA Intruments SpA | Stani G.,SRA Intruments SpA | And 4 more authors.
Journal of Chromatography A | Year: 2015

In this study, the first capillary flow technology reverse-inject differential flow modulator was implemented with different column configurations (lengths, diameters and stationary phase coupling) and detector combinations (mass spectrometry - MS and flame ionization detection - FID) to evaluate its potential in the quantitative profiling and fingerprinting of medium-to-highly complex essential oils. In particular, a parallel dual-secondary column dual-detection configuration that has shown to improve the information potential also with thermally modulated GC×GC platforms (MS identification reliability and accurate FID quantitation), was tested. Several system performance parameters (separation measure S GC×GC, modulation ratio M R , separation space used and peak symmetry) were evaluated by analyzing a mixture of volatiles of interest in the flavor and fragrance field. The systems demonstrating the best chromatographic performance were selected for quantitative profiling of lavender and mint essential oils and fingerprinting of vetiver essential oil. Experimental results demonstrate that careful tuning of column dimensions and system configurations yields improved: (a) selectivity; (b) operable carrier gas linear velocities at close-to-optimal values; (c) 2D separation power by extending the modulation period and (d) handling of overloaded peaks without dramatic losses in resolution and quantitative accuracy. © 2015 Elsevier B.V.


In this study, the first capillary flow technology reverse-inject differential flow modulator was implemented with different column configurations (lengths, diameters and stationary phase coupling) and detector combinations (mass spectrometry--MS and flame ionization detection--FID) to evaluate its potential in the quantitative profiling and fingerprinting of medium-to-highly complex essential oils. In particular, a parallel dual-secondary column dual-detection configuration that has shown to improve the information potential also with thermally modulated GC GC platforms (MS identification reliability and accurate FID quantitation), was tested. Several system performance parameters (separation measure SGC GC, modulation ratio MR, separation space used and peak symmetry) were evaluated by analyzing a mixture of volatiles of interest in the flavor and fragrance field. The systems demonstrating the best chromatographic performance were selected for quantitative profiling of lavender and mint essential oils and fingerprinting of vetiver essential oil. Experimental results demonstrate that careful tuning of column dimensions and system configurations yields improved: (a) selectivity; (b) operable carrier gas linear velocities at close-to-optimal values; (c) (2)D separation power by extending the modulation period and (d) handling of overloaded peaks without dramatic losses in resolution and quantitative accuracy.


PubMed | University of Turin, Agilent Technologies, University of Nebraska - Lincoln and SRA Intruments SpA
Type: | Journal: Journal of chromatography. A | Year: 2016

The possibility to transfer methods from thermal to differential-flow modulated comprehensive two-dimensional gas chromatographic (GCGC) platforms is of high interest to improve GCGC flexibility and increase the compatibility of results from different platforms. The principles of method translation are here applied to an original method, developed for a loop-type thermal modulated GCGC-MS/FID system, suitable for quali-quantitative screening of suspected fragrance allergens. The analysis conditions were translated to a reverse-injection differential flow modulated platform (GC2GC-MS/FID) with a dual-parallel secondary column and dual detection. The experimental results, for a model mixture of suspected volatile allergens and for raw fragrance mixtures of different composition, confirmed the feasibility of translating methods by preserving

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