Pābna, Bangladesh
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Momin M.A.M.,Jahangirnagar University | Momin M.A.M.,Square Pharmaceuticals Ltd | Rashid M.M.,Square Pharmaceuticals Ltd | Urmi K.F.,Jahangirnagar University | Rana M.S.,Jahangirnagar University
Research Journal of Pharmacy and Technology | Year: 2013

The present study was undertaken to investigate the antioxidant and cytotoxicity potential of methanol and petroleum ether extracts of the Trichosanthes dioica roxb. (PM, PE), Benincasa hispida cong (CM, CE), Trichosanthes cucumerina linn. (CHM, CHE) plants. For phytochemical screening, crude extract was tested for the presence of different chemical groups. In Trichosanthes dioica roxb, presence of alkaloids, saponins and flavonoids for methanol extract and carbohydrate, alkaloids are for Pet ether extract were identified. Carbohydrates, steroids, alkaloids, saponins and tannins are present in the methanol extract of Benincasa hispida cong; the pet ether extract was found to have carbohydrates, steroids and tannins. The methanolic extract of Trichosanthes cucumerina linn was found to have carbohydrates, steroids, alkaloids, tannins and glycosides. Carbohydrates, steroids, alkaloids, saponins and glycosides were present in the pet ether of this plant. In vitro antioxidant activity of the extracts were performed using DPPH radical scavenging, total antioxidant capacity, total phenol content, total flavonoid content, nitric oxide (NO) scavenging, and cupric reducing antioxidant capacity assays. The most prominent antioxidant activity was observed with the CHM in the DPPH free radical scavenging activity with an IC50 value of 858.41±30.12729 μg/ml in comparison with that of standard ascorbic acid (IC50 value of 15.707± 1.181μg/ml. In total antioxidant capacity method, PE showed the highest activity (873.3±60.38692 mg ascorbic acid/g). The total phenolic and flavonoids content were determined by Folin-Ciocalteu Reagent and aluminum chloride colorimetric method respectively. The highest total phenols and total flavonoids content were found in PM with the value of 259.375±61.87184 mg gallic acid/g and 52.95±0.212132 mg quercetin/g, respectively. In nitric oxide (NO) scavenging, the most prominent antioxidant activity was observed in CE with IC50 value of 1021.9± 16.235 μg/ml. The Cupric reducing capacity of the extracts were strong and dose dependent manner, and PE showed very good dose depended reducing capacity that is comparable to ascorbic acid. The cytotoxicity was determined using brine shrimp lethality bioassay. The most potent cytotoxicity was shown by CHM with LC50 value 2.59 μg/ml among all the tested extracts. The comparison of different plant extracts used in the present study in various tests showed wide variation in phenolic content and varying degrees of radical scavenging and reducing capacity. The obtained results indicate that the investigated plants could be potential sources of natural antioxidants and can be used for various types of diseases. © RJPT.


Ullah M.,University of Development Alternative | Ullah M.,SQUARE Pharmaceuticals Ltd
American Journal of Pharmacology and Toxicology | Year: 2015

Water For Injections (WFI) which is the main vehicle of Large Volume Parenterals (LVPs) should be free from trace amount of genotoxic impurities. This review gives emphasis on quantification of genotoxic trace metals during qualification of WFI in LVPs manufacturing unit. According to ICH guidelines, impurities related to drug substances are classified into three main categories: Organic impurities, inorganic (elemental) impurities and residual solvents. Within these categories, genotoxic impurities form a special case that poses a significant safety risk, even at low concentrations, because they may be mutagenic and are therefore potentially damaging to DNA. As a result they can lead to mutations or cause cancer. Chemical carcinogens most often directly or after xenobiotic metabolism, act as genotoxic causes to induce DNA damage. The roles of trace metals (some of which are either genotoxic or non-genotoxic) in cancer development and inhibition have a complex character and have raised many questions because of their essential and toxic effects on people’s health. Trace metals such as cadmium, nickel, arsenic, beryllium and chromium (VI) have been recognized as human or animal carcinogens by International Agency for Research on Cancer (IARC). There are several genotoxic chemicals like residual solvent, impurities and trace metals present in Pharmaceuticals to form carcinogens. Regulatory body like FDA and EMEA has fixed up specific limits for these elemental impurities. The toxicity of an elemental impurity is related to its extent of exposure (bioavailability). In that sense, parenteral dosage forms has its most possibility to be bioavailable than the other dosage forms, the limit of genotoxic impurities are 10 times lower than that of oral dosage forms by United States Pharmacopoeia (USP). The present article is for the importance of identification and quantification of the trace amount of the metal genotoxic impurities in Water For Injection (WFI) during qualification (IQ, OQ, PQ) as a preventive measure to control the production and distribution of WFI for Large Volume Parenteral (LVP) production. © 2015 Mohabbat Ullah.


Zahan R.,Atish Dipankar University of Science and Technology | Badrul Alam M.,Atish Dipankar University of Science and Technology | Saiful Islam M.,International Islamic University Chittagong | Sarker G.C.,Square Pharmaceuticals Ltd | And 5 more authors.
International Journal of Cancer Research | Year: 2011

The research study was conducted to determine the antitumor effect of the flower of Alangium salvifolium (crude extract and diethylether fractions) against Ehrlich Ascites Carcinoma (EAC) in mice at the doses of 10 mg kg-1 body weight intraperitoneally. Extract/fractions was administered for nine consecutive days. Twenty-four hours of last dose and 18 h of fasting, the mice were sacrificed and antitumor effect was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight and hematological parameters of EAC bearing host. Significant (p<0.001) increases of survival times 30±0.96 and 25±0.40 days for crude extract and diethylether fraction of the A. salvifolium (10 mg kg-1) treated tumor bearing mice, respectively were confirmed with respect to the control group (20±0.13 days). The extract/fraction also decreased the body weight of the EAC tumor bearing mice. Hematological studies reveal that the heamoglobin (Hb) content was decreased in EAC treated mice whereas restoration to near normal levels was observed in extract treated animals. There was a significant (p<0.001) decrease in RBC count and increase in WBC counts in extract/fraction treated animals when compared to EAC treated animals. From the result it was showed that the extract has significant anticancer activity and that is comparable to that of Bleomycin. © 2011 Academic Journals Inc.


Saiful Islam M.,International Islamic University Chittagong | Badrul Alam M.,Atish Dipankar University of Science and Technology | Zahan R.,Atish Dipankar University of Science and Technology | Sarker G.C.,Square Pharmaceuticals Ltd | And 7 more authors.
International Journal of Cancer Research | Year: 2011

The present study was designed to investigate the antioxidant and anti neoplastic activities of Ocimum sanctum Linn. (OS) leaves. Antioxidant potential of the OS extract was evaluated in vitro by DPPH (1, 1diphenyl-2-picrylhydrazyl) and NO (Nitric Oxide) scavenging assay and reducing power assay method. OS extract showed prominent scavenging activity in all the methods with IC50 value of 16.39±0.31 and 16.20±0.33 μg mL-1 for DPPH and NO assay method, respectively. In reducing power assay, OS extract also showed significant (p<0.001) activity. In addition, total phenolic and flavonoid content and total antioxidant capacity were also determined. The anti neoplastic effect of the leaves of OS against Ehrlich Ascites Carcinoma (EAC) in mice at the doses of 50 mg kg-1 body weight intraperit one ally. Significant (p<0.001) increases of survival times 33±1.81 days for crude extract of the (50 mg kg-1) treated tumor bearing mice were confirmed with respect to the control group (20±0.12 days). Hematological studies reveal that the Heamoglobin (Hb) content was decreased in EAC treated mice whereas restoration to near normal levels was observed in extract treated animals. There was a significant (p<0.001) decrease in RBC count and increase in WBC counts in extract/fraction treated animals when compared to EAC treated animals. From the result it was showed that the extract has significant antioxidant as well as anti neoplastic activity. © 2011 Academic Journals Inc.


Subhan M.A.,Bangladesh University | Subhan M.A.,Osaka Kyoiku University | Uddin N.,Bangladesh University | Sarker P.,Square Pharmaceuticals Ltd. | And 2 more authors.
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | Year: 2015

Abstract A novel multi-metal nanocomposite oxide Ag2O·CeO2·ZnO has been prepared by co-precipitation of their carbonates from aqueous solutions of the metal nitrates following calcinations and annealing 5 h at 450 °C and 4 h at 600 °C. Ag2O·CeO2·ZnO has been characterized by XRD, SEM, EDS and PL spectra. According to XRD results the crystallite size of Ag2O·CeO2·ZnO varies in the range of 19-111 nm with an average size of 50 nm, which is in good agreement with SEM results. Elemental analysis was performed by SEM-EDS. Emissions of Ag2O·CeO2·ZnO has been observed in UV (NBE emission), visible and NIR regions at 325 nm excitation by a line of He-Cd laser. Photocatalytic as well as anti-bacterial activities have been studied. The nano composite Ag2O·CeO2·ZnO shows an excellent photocatalytic dye degradation activity. © 2015 Elsevier B.V.


Subhan M.A.,Bangladesh University | Uddin N.,Bangladesh University | Sarker P.,Square Pharmaceuticals Ltd. | Azad A.K.,Bangladesh University of Engineering and Technology | Begum K.,Bangladesh University of Engineering and Technology
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy | Year: 2015

A novel tri-metallic oxide nanocomposite CeO2·CuO·ZnO has been synthesized by a simple co-precipitation method. The nanocomposite has been characterized by XRD, SEM, EDS, FTIR and PL spectra. The crystallite size of the CeO2·CuO·ZnO was calculated using XRD data. The crystallite size of the CeO2·CuO·ZnO mixed metal oxide annealed at 600 °C is found to be in range of 15.34-44.81 nm, with an average size of 29.51 nm. Excitation at different wavelengths showed PL in UV and visible regions. It has been found that PL behavior of CeO2·CuO·ZnO is excitation wavelength dependent. This flexible PL property is conflicting to well-known Kasha's rule of excitation wavelength dependence of emission spectrum. The catalyst shows better photo-catalytic dye degradation efficiency in slightly alkaline pH in presence of H2O2. Nanocomposite CeO2·CuO·ZnO was found to be effective against pathogenic bacteria. © 2015 Elsevier B.V. All rights reserved.


Huque A.,East West University of Bangladesh | Huque A.,Square Pharmaceuticals Ltd | Biswas S.,Jahangirnagar University | Biswas S.,Square Pharmaceuticals Ltd | And 7 more authors.
International Journal of Pharmaceutical and Clinical Research | Year: 2015

The aim of the study to investigate the analgesic, anti-inflammatory and anxiolytic effect of the methanolic extract obtained from Solanum surattense leaves in mice to authenticate the scientific base of traditional use. Methanol were used to extract the phytochemicals. Separation of compounds was done through gravity column chromatography and Observation of TLC Plate for methanol extract was performed with Naked Eye, Under UV light and applied iodine vapor depending on Rf value for the marking of Fluorescent spots. Methanol extract of Solanum surattense leaves was selected for analgesic, anti-inflammatory and anxiolytic activity. Analgesic and anti-inflammatory were measured by acetic acid-induced writhing inhibition test and xylene induced ear edema test. Open field test (OFT), Elevated plus maze (EPM), Hole cross test and Hole board test were the screening tests used to assess the anxiolytic activity on mice. The crude dried methanol extract was prepared in doses of 100 and 200 mg/kg body weight administered orally to the mice. Extract of Solanum Surattense statistically showed noticeable analgesic as well as anti-inflammatory activity against control. But this extract did not ensure anxiolytic and tranquilizing activity except at high doses(2x) on total ambulation criteria during open field test and at low doses (1x) on close arm in elevated plus maze test against control. The report from studies hints the traditional uses of this plant as analgesic and anti-inflammatory. Further investigation including specific compound isolation of extract can claim the scientific basis as analgesic and anxiolytic agent. © 2015, International Journal of Pharmaceutical and Clinical Research. All rights reserved.


Ur Rashid M.H.,Khulna University | Ur Rashid M.H.,Square Pharmaceuticals Ltd | Biswas S.,Square Pharmaceuticals Ltd | Biswas S.,Jahangirnagar University | And 6 more authors.
Asian Journal of Pharmaceutical and Clinical Research | Year: 2015

Introduction: Pithcellobium dulce (Roxb.) benth, tree 5-18 m high, with pendulous branches, short, sharp stipular spines. It belongs to Fabaceae family. This plant’s accession number is 31,388 as per Bangladesh national herbarium. Methods: For the investigation of its phytochemical and biological activities, the concentrated ethanolic extract of the leaf were extracted. Objective: This crude extract was tested for the presence of various chemical groups and alkaloid, flavonoids, saponin and reducing sugar were identified. The traditional medicinal use of this plant prompted us to check analgesic, anti-bacterial, anti-diarrheal and cytotoxic effects. Result and Discussion: The ethanol extract of the leaf produced 29.4% and 61.54% protection or writhing inhibition in mice at orally dosage of 250 mg/kg (p<0.001) and 500 mg/kg (p<0.001) body weight of mice, respectively. This was comparable to the standard drug diclofenac sodium at the dose of 25 mg/kg of body weight. This extract increased mean latent period and decreased the frequency of defecation significantly at the oral dose of 500 mg/kg body weight, which is also comparable to standard loperamide at dose of 50 mg/kg body weight. This leaf extract also showed antimicrobial activity only against shigella dyst-1 among 10 bacterial species culture. In addition, it also showed cytotoxic effect-50% (LC 50) mortality of Brine Shrimp nauplii occurred at 20 µg/ml and 90% (LC90) mortality at 80 µg/ml ethanolic leaves crude extract. Conclusion: Most of the medicinal plants of Bangladesh have more or less biological activity. It was a preliminary study; further study can be done for the fulfillment of this experiment based on different extract concentration. © 2015, Asian Journal of Pharmaceutical and Clinical Research. All rights reserved.


PubMed | Square Pharmaceuticals Ltd., Bangladesh University of Engineering and Technology and Bangladesh University
Type: | Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy | Year: 2015

A novel tri-metallic oxide nanocomposite CeO2CuOZnO has been synthesized by a simple co-precipitation method. The nanocomposite has been characterized by XRD, SEM, EDS, FTIR and PL spectra. The crystallite size of the CeO2CuOZnO was calculated using XRD data. The crystallite size of the CeO2CuOZnO mixed metal oxide annealed at 600 C is found to be in range of 15.34-44.81 nm, with an average size of 29.51 nm. Excitation at different wavelengths showed PL in UV and visible regions. It has been found that PL behavior of CeO2CuOZnO is excitation wavelength dependent. This flexible PL property is conflicting to well-known Kashas rule of excitation wavelength dependence of emission spectrum. The catalyst shows better photo-catalytic dye degradation efficiency in slightly alkaline pH in presence of H2O2. Nanocomposite CeO2CuOZnO was found to be effective against pathogenic bacteria.

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