Nitra, Slovakia
Nitra, Slovakia

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Krockova J.Z.,SPU v Nitre | Massanyi P.,SPU v Nitre | Sirotkin A.V.,CVZV | Pivko J.,CVZV | And 5 more authors.
Journal of Trace Elements in Medicine and Biology | Year: 2011

The present study was aimed at investigating effects of nickel (NiCl2) on secretion of testosterone (T), cell viability, ultrastructure and apoptosis in mouse Leydig cells. Testosterone release was measured after 48h of culture with 15.67, 31.25, 62.5, 125, 250, 500 and 1000μmol/L NiCl2 or without NiCl2 using radioimmunoassay. Cell viability was assessed by a MTT (metabolic activity assay). Quantification of apoptotic cells was performed using TUNEL assay and the ultrastructural changes were analyzed using transmission electron microscopy. The viability was decreased after addition of ≥250μmol/L NiCl2. A concentration-dependent depression of T production was observed. The percentage of apoptotic cells was significantly increased only after addition of 125, 250 and 1000μmol/L NiCl2. After addition of ≥250μmol/L NiCl2 higher incidence of euchromatin was observed. Lipid droplets and vacuoles in cytoplasm were increased after addition of ≥125μmol/L NiCl2. NiCl2 induced decrease in numbers of mitochondria and smooth endoplasmic reticulum after treatment with ≥500μmol/L NiCl2. Our findings suggest a negative effect of NiCl2 on steroidogenesis, viability, apoptosis and ultrastructure of mouse Leydig cells. © 2010 Elsevier GmbH.


PubMed | SPU v Nitre
Type: Journal Article | Journal: Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) | Year: 2011

The present study was aimed at investigating effects of nickel (NiCl(2)) on secretion of testosterone (T), cell viability, ultrastructure and apoptosis in mouse Leydig cells. Testosterone release was measured after 48h of culture with 15.67, 31.25, 62.5, 125, 250, 500 and 1000mol/L NiCl(2) or without NiCl(2) using radioimmunoassay. Cell viability was assessed by a MTT (metabolic activity assay). Quantification of apoptotic cells was performed using TUNEL assay and the ultrastructural changes were analyzed using transmission electron microscopy. The viability was decreased after addition of 250mol/L NiCl(2). A concentration-dependent depression of T production was observed. The percentage of apoptotic cells was significantly increased only after addition of 125, 250 and 1000mol/L NiCl(2). After addition of 250mol/L NiCl(2) higher incidence of euchromatin was observed. Lipid droplets and vacuoles in cytoplasm were increased after addition of 125mol/L NiCl(2). NiCl(2) induced decrease in numbers of mitochondria and smooth endoplasmic reticulum after treatment with 500mol/L NiCl(2). Our findings suggest a negative effect of NiCl(2) on steroidogenesis, viability, apoptosis and ultrastructure of mouse Leydig cells.

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