Zalejska-Fiolka J.,Medical University of Silesia, Katowice |
Wielkoszynski T.,Medical University of Silesia, Katowice |
Rokicki W.,Medical University of Silesia, Katowice |
Dabrowska N.,Medical University of Silesia, Katowice |
And 8 more authors.
BioMed Research International | Year: 2015
We hypothesized that addition of substances with antioxidant activity could decrease the concentrations of biomarkers of oxidative stress and inflammatory process, thus inhibiting nonalcoholic steatohepatitis development. We investigated the influence of lipoic acid (ALA) and garlic administration on the development of adverse changes in rabbit liver and serum under oxidative stress conditions induced with HFD from oxidized oils. We determined 8-hydroxy-2′-deoxyguanosine (8OHdG) and malondialdehyde (MDA) in liver homogenates, total oxidant status (TOS), lipid peroxides (LOO) and tumor necrosis factor alpha (TNF) in blood serum, and TNF and IL-1 genes expression in liver. The results indicate that the intake of dietary ALA and garlic was significantly associated with decreases of 8OHdG and MDA levels in rabbits' liver tissue as well as TOS and LOO levels in rabbits' serum. Similarly, TNF and IL-1 gene expressions were suppressed due to ALA and garlic supplementation. The histopathological analysis confirmed that HFD results in liver disorder leading to steatosis. This adverse effect of HFD was ameliorated by the supplementation of ALA and garlic. The obtained results indicate a beneficial effect of ALA and garlic administration by reducing the oxidative stress intensity and the levels of some proinflammatory cytokines in rabbits fed HFD. © 2015 Jolanta Zalejska-Fiolka et al. Source
Rychter P.,Jan Dlugosz University in Czestochowa |
Pamula E.,AGH University of Science and Technology |
Orchel A.,SPLMS in Sosnowiec |
Posadowska U.,AGH University of Science and Technology |
And 10 more authors.
Journal of Biomedical Materials Research - Part A | Year: 2015
The aim of the presented study was preparation, analysis of properties, and in vitro characterization of porous shape-memory scaffolds, designed for large bone defects treatment using minimally invasive surgery approach. Biodegradable terpolymers of l-lactide/glycolide/trimethylene carbonate (LA/GL/TMC) and l-lactide/glycolide/ε-caprolactone (LA/GL/Cap) were selected for formulation of these scaffolds. Basic parameters of shape memory behavior (i.e. recovery ratio, recovery time) and changes in morphology (SEM, average porosity) and properties (surface topography, water contact angle, compressive strength) during shape memory cycle were characterized. The scaffolds preserved good mechanical properties (compressive strength about 0.7 to 0.9 MPa) and high porosity (more than 80%) both in initial shape as well as after return from compressed shape. Then the scaffolds in temporary shape were inserted into the model defect of bone tissue at 37°C. After 12 min the defect was filled completely as a result of shape recovery process induced by body temperature. The scaffold obtained from LA/GL/TMC terpolymer was found the most prospective for the planned application thanks to its appropriate recovery time, high recovery ratio (more than 90%), and cytocompatibility in contact with human osteoblasts and chondrocytes. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3503-3515, 2015. © 2015 Wiley Periodicals, Inc. Source