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Cambridge, United Kingdom

Bakewell D.J.,University of Liverpool | Bailey J.,University College London | Holmes D.,University College London | Holmes D.,Sphere Fluidics
Materials Today: Proceedings | Year: 2016

Nanoparticle interactions with micron-scale environments can be induced using dielectrophoresis (DEP). A new DEP signaling and additive noise model is developed from first principles. It is applied to fluorescence microscope CCD video images of fluorescently labeled, 200 nm diameter, latex nanospheres moved by pulsed, positive DEP (pDEP) onto 5 micron planar castellated electrode edges. The model increases the signal-to-noise ratio (SNR) for the time dependent spatial mean of the video frame fluorescence intensity and thereby enables correlation with the spatial standard deviation (std) statistic. Deterministic pDEP-induced collections are approximately linearly correlated, whereas thermally-driven, random, nanoparticle movements are nonlinearly correlated. © 2016 Elsevier Ltd. Source


Duellberg C.,The Francis Crick Institute | Cade N.I.,The Francis Crick Institute | Holmes D.,Sphere Fluidics | Surrey T.,The Francis Crick Institute
eLife | Year: 2016

The function of microtubules relies on their ability to switch between phases of growth and shrinkage. A nucleotide-dependent stabilising cap at microtubule ends is thought to be lost before this switch can occur; however, the nature and size of this protective cap are unknown. Using a microfluidics-assisted multi-colour TIRF microscopy assay with close-to-nm and sub-second precision, we measured the sizes of the stabilizing cap of individual microtubules. We find that the protective caps are formed by the extended binding regions of EB proteins. Cap lengths vary considerably and longer caps are more stable. Nevertheless, the trigger of instability lies in a short region at the end of the cap, as a quantitative model of cap stability demonstrates. Our study establishes the spatial and kinetic characteristics of the protective cap and provides an insight into the molecular mechanism by which its loss leads to the switch from microtubule growth to shrinkage. © Duellberg et al. Source


Chokkalingam V.,Radboud University Nijmegen | Tel J.,Radboud University Nijmegen | Wimmers F.,Radboud University Nijmegen | Liu X.,Sphere Fluidics | And 4 more authors.
Lab on a Chip - Miniaturisation for Chemistry and Biology | Year: 2013

Here, we present a platform to detect cytokine (IL-2, IFN-γ, TNF-α) secretion of single, activated T-cells in droplets over time. We use a novel droplet-based microfluidic approach to encapsulate cells in monodisperse agarose droplets together with functionalized cytokine-capture beads for subsequent binding and detection of secreted cytokines from single cells. This method allows high-throughput detection of cellular heterogeneity and maps subsets within cell populations with specific functions. © 2013 The Royal Society of Chemistry. Source


Grant
Agency: GTR | Branch: Innovate UK | Program: | Phase: Collaborative Research & Development | Award Amount: 234.41K | Year: 2014

Sphere Fluidics Limited is an SME focusing on the development of novel, single cell analysis technologies. MedImmune, the global biopharmaceuticals arm of AstraZeneca, is a pioneer in biopharmaceutical discovery and development. Both companies have pooled their synergistic, science skills to propose the development of a unique, integrated microfluidic instrument that uses miniaturisation and novel chips to process pools of cells and rapidly isolate single cells for applications in biopharmaceutical research and development.


Patent
CAMBRIDGE ENTERPRISE Ltd and Sphere Fluidics | Date: 2011-08-17

This invention relates to methods and systems for providing chemical and/or biological materials. Thus we describe a method of providing a chemical or biological material in a quantised form, the method comprising: preparing an emulsion comprising a plurality of microdroplets each holding a controlled amount of said material; storing said chemical or biological material in said microdroplets in said emulsion; transferring a portion of said emulsion microdroplets to a container for transportation; transporting said container to a user of said material, wherein said user receives said material in a quantised form in said microdroplets of said emulsion; and de-emulsifying said received microdroplets containing said material in quantised form to extract said material for subsequent use.

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