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Gaukhar A.,South Kazakhstan State University | Alibayev N.,South Kazakhstan State University | Alibayev N.,Southwestresearch Institutes of Animal Husbandry | Saparbekova A.A.,South Kazakhstan State University | And 2 more authors.
Life Science Journal | Year: 2014

The effective way of selection of pregnant mares as producers of the gonadotropic hormones, promoting improvement of quality of preparation of a preparation of pregnant mare serum with high hormonal activity and an optimum ratio of FSH/LH is developed. Biological qualities of pregnant mare serum of different age and pregnancy terms - hormonal activity, a ratio of follicle-stimulating and luteinizing hormones for preparation of a hormonal biological product of PMS (pregnant mare serum) with the use of methods of diagnostics of a pregnancy of mares on mature males of lake frogs, and the immunofermental analysis for definition of a ratio of hormones in blood plasma are investigated. On a variety of activity of PMS and FSH and LH ratio in blood serum essential influence renders to the age of donor mares and their terms of a pregnancy. The influence of age of pregnant mares on FSH/LH ratio in blood serum, received from them is 0.107±0.046 with reliability at level of the first probability threshold (P<0.05), and influence terms of a pregnancy of donors on level of concentration of gonadotropic hormones in serum is authentic above at the significance of P<0.001 (0.447±0.033). The influence of age of donors on activity of PMS and on FSH/LH ratio in serum is not more than 9.9 % and 20.4 % respectively, and influence of FSH/LH ratio on activity of PMS is not less than 63.6 % and not more than 73.2 %. On the basis of the carried-out pilot studies the new way of selection of pregnant mares as producers of gonadotropic hormones is developed for production of PMS with the high activity. Thus, the optimum day for blood draw from 3-5 year donor mares is considered between 65- 80 days of pregnancy. For donors of 6-8 years the best term of the period is from 60 to 75 day and for advanced age of 9 and 10 years is from 55 to 65th day of pregnancy.

Gaukhar A.,South Kazakhstan State University | Nuraddin A.,South Kazakhstan State University | Nuraddin A.,Southwestresearch Institutes of Animal Husbandry | Svitous A.,Baltic Foundation
Advances in Environmental Biology | Year: 2014

The new method of receiving gonadotrophins from serum of pregnant mares is based on methods of settling of ballast proteins and gonadotrophins, determination of its hormonal activity, a ratio of follicle stimulating and lutheinizing hormones in a preparation, differing by that 3-5 donor mares with term of pregnancy of 65-85 days, 6-8 year donor mares with term of a pregnancy of 60-75 days and 9-10 year donors with term of 55-65 days are selected as producers of PMSG. This development provides a high outcome of a gonadothropic hormone from initial raw materials and stabilizes their variability. © 2014 AENSI Publisher All rights reserved.

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