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Xu J.,Kyushu University | Zhang P.,Kyushu University | Kusakabe T.,Kyushu University | Mon H.,Kyushu University | And 6 more authors.
Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics

Abstract We reported previously that baculovirus AcMNPV host-ranges in silkworm strains are controlled by a novel third chromosomal locus. To further isolate the potential host factor and uncover the functional pathway involved, in this study we analyzed hemolymph proteins from AcMNPV-resistant or -sensitive silkworm strains infected with baculoviruses. All the protein spots from 2D electrophoresis were characterized by MALDI-TOF MS and further systematically assessed for differentially regulated proteins at different stages of infection. Subsequently, six candidates were selected for functional analysis using Bm5 cells, where the candidates were knocked-down or overexpressed. We observed that mRNA expression levels of beta-N-acetylglucosaminidase and prophenoloxidase subunit 2 are significantly upregulated during AcMNPV infections in Bm5 cells. Ultimately, we found that RNA interference of ribosomal protein RpL34 causes serious damages to cell viability as well as abortive infection, indicating that ribosomal components are essential for productive baculovirus infection. © 2015 Elsevier Inc. Source

Li Q.,Chongqing Medical and Health School | Tan X.,Southwest UniversityChongqing | Zheng X.,Chongqing Medical and Health School | Tang W.,Chongqing Medical and Health School | And 2 more authors.
Journal of Molecular Structure

Abstract A novel resonance Rayleigh scattering method was developed for the determination of fast green (FCF) in grape wine. In pH 2.5 Britton Robinson (BR) buffer solution, the scattering signal of acridine orange (AO) was remarkably enhanced after adding trace amount of FCF and forming an ion-association complex, which not only resulted in the change of absorption spectrum, fluorescence spectra, but also led to a significant enhancement of resonance Rayleigh scattering (RRS), frequency doubling scattering (FDS), and second order scattering (SOS). The linear ranges and detection limits for RRS, SOS and FDS were 2-45 × 10-6 mol L-1, 2-24 × 10-6 mol L-1, 2-20 × 10-6 mol L-1, and 8.0 × 10-8 mol L-1, 4.7 × 10-7 mol L-3, 1.0 × 10-7 mol L-3, respectively. In this work, the optimum conditions, the influencing factors and the effects of coexisting substances on the reaction were investigated. The method can be applied to the determination of FCF in grape wine and the results were satisfactory. © 2015 Elsevier B.V. Source

Peng X.,Southwest UniversityChongqing | Chu J.,CAS Chongqing Institute of Green and Intelligent Technology | Aldalbahi A.,King Saud University | Rivera M.,University of Puerto Rico at San Juan | And 3 more authors.
Applied Surface Science

Multi-walled carbon nanotubes (MWCNTs) and single-walled carbon nanotubes (SWCNTs) were dispersed in the biopolymer kappa-carrageenan (KC) to form a flexible composite via evaporative casting method. The glycerin was used as plasticizer to increase the flexibility of the composite. The KC—CNTs was examined by using FESEM and Raman, and then the humidity sensing properties of the samples were characterized under alternating current (AC). The purpose for using AC power supply is to avoid the possible polarization effect during measurements of the humidity properties. The experimental data exhibit that the fabricated sensors have high response to relative humidity (RH) with good repeatability, stability, and low hysteresis. A phenomenon that the impedance of the sensor decreases with ascending RH was also found and the basic sensing mechanisms were discussed. © 2016 Elsevier B.V. Source

Luo Y.,Southwest UniversityChongqing | Miao H.,Southwest UniversityChongqing | Yang X.,Southwest UniversityChongqing

Abstract Glutathione (GSH), playing roles as both a reducing reagent and protecting ligand, has been successfully employed for synthesizing Cu nanoclusters (CuNCs@GSH) on the basis of a simple and facile approach. The as-prepared CuNCs exhibited a fluorescence emission at 600 nm with a quantum yield (QY) of approximately 3.6%. Subsequently, the CuNCs described here was employed as a broad-range pH sensor by virtue of the fluorescence intensity of CuNCs responding sensitively to pH fluctuating in a linear range of 4.0-12.0. Meanwhile, these prepared CuNCs were applied for detections of vitamin B1 (VB1) on the basis of positively charged VB1 neutralizing the negative surface charge of CuNCs, thus leading to the instability and aggregations of CuNCs, and further facilitating to quench their fluorescence. In addition, the proposed analytical method permitted detecting VB1 with a linear range of 2.0×10-8-1.0×10-4 mol L-1 as well as a detection limit of 4.6×10-9 mol L-1. Eventually, the practicability of this sensing approach was validated by assaying VB1 in human urine samples and pharmaceutical tablets, confirming its potential to broaden avenues for assaying VB1. © 2015 Elsevier B.V. Source

Li S.-K.,Southwest UniversityChongqing | Chen A.-Y.,Southwest UniversityChongqing | Chai Y.-Q.,Southwest UniversityChongqing | Yuan R.,Southwest UniversityChongqing | Zhuo Y.,Southwest UniversityChongqing
Electrochimica Acta

In this work, a novel and sensitive electrochemiluminescence (ECL) aptasensor for mucin 1 (MUC1) was designed based on cascading amplification of nicking endonuclease-assisted target recycling and rolling circle amplifications (RCA). In the presence of target MUC1, Nt.BbvC I-assisted target recycling process generated massive single-stranded primers which were available for subsequent RCA reactions on the as-prepared sensing interface. With rationally sequence designed, massive long DNA molecules with multiple G-quadruplex units were formed to generate the G-quarduplex/hemin complexes in the presence of hemin, which could enhance the ECL efficiency of luminol-H2O2 system due to its strongly catalytic performance toward the coreactant H2O2. The proposed aptasenor for MUC1 detection showed a limit of detection down to 0.71 fg/mL and a wide linear range from 10 fg/mL to 10 ng/mL with acceptable stability, sensitivity, and possessed promising potential applications in clinical diagnostics. More significantly, it would be available for generalizing the proposed strategy to detect a series of other relative biomarkers. © 2016 Elsevier Ltd Source

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