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Yao X.-Y.,Southern District Hospital of Manzhouli | Yang L.-M.,Inner Mongolia University | Hou J.-F.,Southern District Hospital of Manzhouli | Hou J.-F.,Inner Mongolia University | And 4 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2014

Background: Under a specific environment in vivo and in vitro, umbilical cord blood mesenchymal stem cells can be induced to differentiate into various tissues and cells, including neural stem cells. Objective: To investigate the safety and efficacy of human umbilical cord blood mononuclear cell transplantation via lumbar puncture in the treatment of delayed encephalopathy after acute carbon monoxide poisoning. Methods: Sixty patients with delayed encephalopathy after acute carbon monoxide poisoning were randomly divided into two groups: treatment group undergoing intrathecal injection of human umbilical cord blood mononuclear cells that were isolated using density gradient centrifugation method into the subarachnoid space of patients with delayed encephalopathy induced by acute carbon monoxide poisoning; control group undergoing hyperbaric oxygen and drug treatments. Mini-mental state examination (MMSE), improved Asworth muscle spasm degree and activity of daily living scale (ADL) were assessed in patients prior to and 3, 9, 12 months after cell transplantation. The MRI changes of the two groups were compared. Meanwhile, lung X-ray, electrocardiogram and blood biochemical test were also measured to evaluate the safety of human umbilical cord blood mononuclear cell transplantation. Results and Conclusion: At 3, 9, 12 month after cell transplantation, the Asworth muscle spasm degree was significantly lower in the treatment group than the control group (P=0.032). At 9 and 12 months after cell transplantation, scores on MMSE and ADL were also higher in the treatment group than the control group (P < 0.05). Change of neurological functions in patients from two groups showed a similar tendency. At 12 months after cell transplantation, MRI examination showed better outcomes in the treatment group than the control group. These findings indicate that intrathecal injection of human umbilical cord blood mononuclear cells is superior to hyperbaric oxygen in the treatment of delayed encephalopathy induced by acute carbon monoxide poisoning. Source

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