Hwang J.Y.,National Institute of Fisheries Science |
Kwon M.G.,National Institute of Fisheries Science |
Jung S.-H.,National Institute of Fisheries Science |
Park M.A.,Southeast Sea Fisheries Research Institute |
And 4 more authors.
Fish and Shellfish Immunology | Year: 2017
Viral hemorrhagic septicemia (VHS) is a highly contagious disease of cultured flounder caused by VHS virus (VHSV). To develop effective VHSV vaccines, it is essential to understand the molecular mechanisms underlying the host's protective response against VHSV. The purpose of this study is to clarify which genes are involved in the protective response of olive flounder after VHSV vaccination. We first injected olive flounder intraperitoneally with 107 TCID50 heat-inactivated VHSV vaccine and evaluated the vaccine efficacy at 20 °C. Fish vaccinated with heat-inactivated VHSV were significantly protected compared to non-vaccinated fish, with a relative percentage survival of 83%. To analyze the vaccination-induced changes in the expression profiles of genes, kidneys were collected from control and vaccinated fish at days 1, 3, and 7 after vaccination and global gene expression profiling was carried out by RNA sequencing. The analysis revealed that 15,001 genes were differentially expressed by at least 2-fold between vaccinated fish and non-vaccinated controls. Of these, 58 genes clustered into the acute phase response, Toll-like receptor, interferon-inducible/regulatory proteins, and apoptosis pathways. These data provided insights into the molecular mechanisms underlying the protective immune response of olive flounder against heat-inactivated VHSV vaccine and might aid future studies to develop a highly immunogenic vaccine against VHSV in flounder. © 2017 Elsevier Ltd
Kim J.N.,West Sea Fisheries Research Institute |
Choi J.H.,Southeast Sea Fisheries Research Institute |
Jo H.-S.,Kunsan National University
Ocean Science Journal | Year: 2016
A single specimen of the pelagic shrimps, Pasiphaea japonica Omori, 1976 (Pasiphaeidae) collected in the southeastern waters of Korea is described and illustrated. Although this species occurs widely in the Indo-West Pacific including the Japanese coast of the East/Japan Sea and the middle and southern parts of the East China Sea, this is the first record of the species and the genus in Korean waters. The species is distinguished from other congeners by the following combination of characteristics: non-carinate dorsal sixth abdominal somite with a terminal tooth, rudimentary pleurobranch on the eighth thoracic somite, merus of the first pereopod with more than eight spines, and almost entirely transparent white color. © 2016, Korea Ocean Research & Development Institute (KORDI) and the Korean Society of Oceanography (KSO) and Springer Science+Business Media Dordrecht.
Kim J.K.,Pukyong National University |
Baek H.-J.,Pukyong National University |
Kim J.-W.,Gangwon Province College |
Chang D.-S.,Subtropical Fisheries Research Center |
Kim J.-I.,Southeast Sea Fisheries Research Institute
Fisheries and Aquatic Science | Year: 2012
Scartelaos gigas is an amphibious mudskipper species that inhabits mud flats in Korea, China, and Taiwan. This fish is at risk of extinction because of its very restricted habitat and overexploitation. Information about this fish's reproductive characteristics is needed for species conservation. The sexual maturity and early life history of S. gigas were investigated through histological methods and direct observation of eggs in the wild, respectively. In total, 560 individuals of S. gigas were collected with the aid of fishermen from March 2003 to October 2003 at Jung-do Island, southwest Korea. Through microscopic observations of gonadal development, it was determined that S. gigas of both sexes were immature in April, but began to reach maturity in May, and were then fully mature by June, which was maintained until July. In August, some female fish developed early oocytes, but by September oocytes were observed to have degenerated and had been absorbed. Spawned eggs were elliptical and had an average size of 1.37 mm (long axis) by 0.69 mm (short axis). The newly hatched larvae (3.03 mm total length, TL) had an open mouth and anus, two melanophores near the anus, and one large melanophore between the 18th and 19th myomeres. The larvae (3.18 mm TL) showed absorption of the yolk and oil globule within 5 days after hatching and became prelarvae. This species should be considered vulnerable or conservation-dependent, and thus parental fish need to be protected from fishermen during the main spawning season (June). © The Korean Society of Fisheries and Aquatic Science.
Choi J.-H.,Southeast Sea Fisheries Research Institute |
Park W.-G.,Pukyong National University
Crustaceana | Year: 2016
Total length, and the length of carapace and telson, are generally used to measure the size of mysid crustaceans. However, this method may not be as accurate for measuring these animals, since mysids commonly bend their abdomen when they are collected and preserved. Besides, the fragile carapace of mysids may result in less accurate outputs in such size measurements. The aim of this study was to determine the relationship between carapace length and statolith diameter in Mysida. The carapace length and statolith diameter were measured in 1199 individuals of Neomysis nigra Nakazawa, 1910. The statolith diameter was significantly correlated with carapace length. It is suggested that the statolith diameter of mysids could be used as an alternative measurement for characterizing body size in these crustaceans. © National Institute of Fisheries Sciences, Korea 2016.
Cepolacanthus kimi, a new genus and species of copepod (Cyclopoida: Taeniacanthidae) parasitic on Bandfish Acanthocepola abbreviata (Valenciennes, 1835) (Actinopterygii: Cepolidae) caught off the Iraqi coast
PubMed | Kyungpook National University, Southeast Sea Fisheries Research Institute, Fountain and University of Basrah
Type: Journal Article | Journal: Zootaxa | Year: 2016
A new genus and species, Cepolacanthus kimi, of the family Taeniacanthidae is established based on adult female specimens collected from the gills of Bandfish Acanthocepola abbreviata (Valenciennes, 1835) (Actinopterygii: Perciformes: Cepolidae) caught off the coast of Iraq. The new genus is characterised by the following combination of features: 1) the urosome is composed of five somites, with the last urosomite probably being a compound somite formed from the failure of the third and fourth abdominal somites to separate; 2) the seta on the maxillary basis is displaced proximally from the free spinulated element; 3) the terminal claw (endopod) of the maxilliped is long and attenuate; 4) the endopod of legs 2-4 is 2-segmented; and 5) the terminal exopodal segment of leg 4 bears a prominent distolateral protuberance.Cepolacanthus kimi gen. et sp. nov. is the first copepod reported from A. abbreviata and the second taeniacanthid species recorded from the Iraqi coast. A new replacement name, Suncheonacanthus nom. nov., is proposed for the preoccupied generic name, Triacanthus Kim & Moon, 2013.
PubMed | Southeast Sea Fisheries Research Institute, Korea Polar Research Institute and Incheon National University
Type: Journal Article | Journal: PloS one | Year: 2016
Antifouling biocides such as organotin compounds and their alternatives are potent toxicants in marine ecosystems. In this study, we employed several molecular and biochemical response systems of the Pacific oyster Crassostrea gigas to understand a potential mode of action of antifouling biocides (i.e. tributyltin (TBT), diuron and irgarol) after exposure to different concentrations (0.01, 0.1, and 1 g L-1) for 96 h. As a result, all the three antifouling biocides strongly induced the antioxidant defense system. TBT reduced both enzymatic activity and mRNA expression of Na+/K+-ATPase and acetylcholinesterase (AChE). Lower levels of both Na+/K+-ATPase activity and AChE mRNA expression were observed in the diuron-exposed oysters compared to the control, while the irgarol treatment reduced only the transcriptional expression of AChE gene. We also analyzed transcript profile of heat shock protein (Hsp) superfamily in same experimental conditions. All antifouling biocides tested in this study significantly modulated mRNA expression of Hsp superfamily with strong induction of Hsp70 family. Taken together, overall results indicate that representative organotin TBT and alternatives have potential hazardous effects on the gill of C. gigas within relatively short time period. Our results also suggest that analyzing a series of molecular and biochemical parameters can be a way of understanding and uncovering the mode of action of emerging antifouling biocides. In particular, it was revealed that Pacific oysters have different sensitivities depend on the antifouling biocides.
PubMed | Jeju National University, Marine Life Research, Southeast Sea Fisheries Research Institute and Korea Institute of Oriental Medicine
Type: | Journal: Fish & shellfish immunology | Year: 2016
Glutathione reductase (GSR) is an enzyme that catalyzes the biochemical conversion of oxidized glutathione (GSSG) into the reduced form (GSH). Since the ratio between the two forms of glutathione (GSH/GSSG) is important for the optimal function of GSH to act as an antioxidant against H
PubMed | Southeast Sea Fisheries Research Institute and Korea Food Research Institute
Type: Journal Article | Journal: Marine pollution bulletin | Year: 2016
From 2011 to 2013, we conducted a full sanitary survey of pollution sources in proximity to a designated shellfish growing area in Korea, and their impact on the sea area therein. From this area, 836 seawater samples and 93 oyster samples were examined to evaluate their bacteriological quality. There were 483 potential pollution sources in the drainage area of the Jaranman-Saryangdo area, including 38 sources discharging water. It demonstrates that while many pollution sources have been identified, no significant impact occurred within the designated shellfish growing area. Variations in fecal coliform (FC) levels in seawater were closely related to rainfall. The FC levels of seawater and oysters from the designated area met the regulation limits set by various countries. Our study indicates that the oysters produced in this area are apparently safe for raw consumption based on their bacterial quality.
PubMed | Southeast Sea Fisheries Research Institute
Type: Journal Article | Journal: Systematic parasitology | Year: 2015
A new species of Anchistrotos Brian, 1906 (Copepoda: Cyclopoida: Taeniacanthidae), parasitic in the branchial cavities of the filamentous shrimpgoby Myersina filifer (Valenciennes) (Perciformes: Gobiidae) from Korea is described. The new species is most closely related to A. tangi Venmathi Maran, Moon & Adday, 2014, but differs from it by the following combination of characters in the adult female: the U-shaped rostrum, the distal margin of the anal somite lacks patches of spinules, the proximal segment of the maxilliped is without seta, and the maxilliped claw is armed with long and small naked setae. This is the tenth species of the genus and a key is provided to distinguish all nominal species.
PubMed | Southeast Sea Fisheries Research Institute
Type: Journal Article | Journal: Bulletin of environmental contamination and toxicology | Year: 2015
From 2009 to 2013, 80 oyster and 16 seawater samples were collected from the southern coast of Korea, including designated shellfish growing areas for export. The concentrations and bioaccumulation of heavy metals were determined, and a potential risk assessment was conducted to evaluate their hazards towards human consumption. The cadmium (Cd) concentration in oysters was the highest of three hazardous metals, including Cd, lead (Pb), and mercury (Hg), however, below the standards set by various countries. The metal bioaccumulation ratio in oysters was relatively high for zinc and Cd but low for Hg, Pb, arsenic, and chromium. The estimated dietary intakes of all heavy metals for oysters accounted for 0.02%-17.75% of provisional tolerable daily intake. The hazard index for all samples was far <1.0, which indicates that the oysters do not pose an appreciable hazard to humans for the metal pollutants of study.