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Ma J.,South China Agricultural University | Ma J.,Sichuan Agricultural University | He Y.-H.,South China Agricultural University | Wu C.-H.,South China Agricultural University | And 3 more authors.
African Journal of Biotechnology | Year: 2012

Initial calli was induced from leaf base of aseptic shoots. These calli were co-cultivated with Agrobacterium tumefaciens strain LBA4404 harboring a plant expression vector pUHA1 containing a human cytochrome P4501A1 (CYP1A1) gene for 3 days. Then, the infected calli were transferred to differentiation medium. Adventitious buds were generated after about 10 day's incubation. The generated shoots were cultured on a medium containing 30 to 50 mg L -1 Km for screening. The selected Km-resistant shoots were subsequently transferred to rooting medium for rooting. We derived 95 Km-resistant plants from four infection groups in total. The transformation frequency was 0.12 to 2.69%. Further analysis by polymerase chain reaction (PCR) and Southern blotting confirmed that the positive frequency of Km-resistant plants was 53.58%. We also confirmed that these protocols below were essential for A. tumefaciens-mediated genetic transformation of pineapple calli: taking agar as medium gelling agent, adding AS to co-culture medium, increasing selection times and gradually increasing the concentration of Km in the selection medium. © 2012 Academic Journals. Source


Li L.,South China Agricultural University | Li L.,Sericulture and Agri Food Research Institute | Wang S.B.,South China Agricultural University | Wang S.B.,South Subtropical Crop Research Institute | And 4 more authors.
Acta Horticulturae | Year: 2013

The contents and their dynamic arrangements of six antioxidants and total antioxidant capacity in the three mango (Mangifera indica L.) cultivars, 'Tainong No.1' ('Haden' × 'Irwin'), 'Yuexi No.1' (the progeny of 'Carabao') and 'Chunhuang' ('White' × 'Keitt'), were estimated at fruit harvest-maturity (harvesting stage) and edible-ripening stages in South China. The results showed that, both at fruit harvesting and edible-ripening stages, total carotenoid contents and total antioxidant capacity were in the order: 'Yuexi No.1'>'Tainong No.1'>'Chunhuang'; the ascorbic acid (AA) content was 'Tainong No.1'>'Yuexi No.1'>'Chunhuang'; total polyphenol content was 'Yuexi No.1'>'Chunhuang'>'Tainong No.1'; reduced glutathione (GSH) content was 'Tainong No.1'>'Chunhuang'>'Yuexi No.1'. At fruit harvesting stage, total flavonoid content was 'Tainong No.1'>'Chunhuang'>'Yuexi No.1' and α-tocopherol content was 'Yuexi No.1'>'Tainong No.1'>'Chunhuang'. And at fruit edible-ripening stage, total flavonoid content was 'Yuexi No.1'>'Tainong No.1'>'Chunhuang' and α-tocopherol content was 'Tainong No.1'>'Yuexi No.1' >'Chunhuang'. In addition, total flavonoid content at edible-ripening stage was significantly higher than that at harvesting stage in each cultivar, and 'Yuexi No.1' possessed a conspicuous high level at this stage. © ISHS 2013. Source


Ma J.,South China Agricultural University | Ma J.,Sichuan Agricultural University | He Y.,South China Agricultural University | Wu C.,South China Agricultural University | And 3 more authors.
Plant Molecular Biology Reporter | Year: 2012

A somatic embryogenesis receptor-like kinase (SERK) gene, designated as AcSERK1, was isolated from pineapple (Ananas comosus cv. Shenwan). AcSERK1 shared all the characteristic domains of the SERK family, including five leucine-rich repeats, one proline-rich region motif, transmembrane domain, and kinase domains. Somatic embryogenic cultures of pineapple were established following transfer of callus cultures to Murashige and Skoog (1962) medium containing 2,4-dichlorophenoxyacetic acid. The role of AcSERK1 during establishment of somatic embryogenesis in culture was investigated. The AcSERK1 was highly expressed during embryogenic competence acquisition and global embryo formation in culture. These findings were obtained along with morphological changes in callus cultures exhibiting embryogenic potential. Overall, levels of expression of AcSERK1 were lower in nonembryogenic tissues and organs than in embryogenic callus. In situ hybridization analysis revealed that AcSERK1 expression was detected in embryogenic tissues, including single competent cells, meristematic centers wherein embryogenic structures are formed, and global embryos. These results suggested that AcSERK1 expression was associated with induction of somatic embryogenesis and that it could be used as a potential marker gene to monitor the transition of pineapple callus tissues into competent and embryogenic cells and tissues. © 2011 Springer-Verlag. Source


He Y.D.,Chinese Academy of Agricultural Sciences | Li R.M.,Institute of Tropical Bioscience and Biotechnology | Wei C.B.,South Subtropical Crop Research Institute | Sun G.M.,South Subtropical Crop Research Institute
Advanced Materials Research | Year: 2014

As an essential element, calcium plays a key role in plant development. The present study aimed at assessing the effects of exogenous calcium on root activity, plant growth and endogenous hormone contents in pineapple seedlings. Major experimental methods included hydroponic culture with six concentrations (0, 5, 10, 20, 40, 80 mg/L) of CaCl2 and the use of high performance liquid chromatography (HPLC) for its endogenous hormone contents. After cultivating for 48 days, the seedlings were sampled and the reduction of TTC (2,3,5-triphenyitetrazolium chloride) method was determined to assess the root activity, and the determination of endogenous hormone contents was carried out by HPLC. The results showed that the shoot fresh weight, the root activity, root length and root weight increased significantly in response to the 20 mg/L Ca2+ treatment, and all these parameters seemed to be suppressed at higher Ca2+ concentrations. The contents of endogenous hormone ZT, GA3 and IAA were evidently higher at 40 mg/L Ca2+, with ZT, GA3 and IAA reach values as high as 2.31, 31.48 and 16.57 μg/g, respectively, while the highest concentration of ABA (0.026 μg/g) appeared at 5 mg /L Ca2+ concentration. © (2014) Trans Tech Publications, Switzerland. Source

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