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Naderi M.,Khorramshahr Marine Science and Technology University | Safahieh A.,Khorramshahr Marine Science and Technology University | Madiseh S.D.,South of Iran Aquaculture Research Center | Zolgharnein H.,Khorramshahr Marine Science and Technology University | Ghatrami E.R.,Khorramshahr Marine Science and Technology University
Toxicology and Industrial Health | Year: 2015

In the present study, the effects of xenoestrogen 4-nonylphenol (4-NP) and 17β-estradiol (E2) on the synthesis of vitellogenin (VTG) in immature male yellowfin seabream (Acanthoparus latus) were assessed to determine the potential use of this species as a biological model for studying endocrine disrupters in the Musa estuary in southern seawaters of Iran. A total number of 104 fish were injected by 10, 50, 100, 150 and 200 μg g-1 week-1 of 4-NP and 2 μg g-1 week-1 of E2, and their blood plasma was obtained for biochemical analysis on days 0, 7 and 14. The hepatosomatic index (HSI) of each fish was also recorded at each sampling time. The results showed that 4-NP significantly induced VTG synthesis in A. latus, which was detected by gel electrophoresis as two major protein bands (∼210 and 191 KDa). The induction of VTG was also determined indirectly by measuring the alkali-labile phosphate, total plasma calcium and protein. All of these VTG indicators significantly increased in the plasma of 4-NP- and E2-treated fish. Meanwhile, a significant dose-dependent increase was observed in HSI, indicating the activation of hepatic VTG production in 4-NP- and E2-injected fish. Furthermore, the plasma alanine aminotransferase and aspartate aminotransferase concentrations significantly increased in 4-NP-treated fish, presumably reflecting a situation of hepatic tissue damage due to 4-NP toxicity. Based on the results, it is suggested that A. latus could be used successfully as a model species for future studies of endocrine disrupting contaminants in the Musa estuary. © The Author(s) 2012.


Dehghan Madiseh S.,South of Iran Aquaculture Research Center | Esmaily F.,South of Iran Aquaculture Research Center | Marammazi J.Gh.,South of Iran Aquaculture Research Center | Koochaknejad E.,South of Iran Aquaculture Research Center | Farokhimoghadam S.,Islamic Azad University at Tehran
Iranian Journal of Fisheries Sciences | Year: 2012

Benthic invertebrates are a well-established target in environmental quality status evaluations. The AMBI (AZTI's Marine Biotic Index) was developed to determine the impacts and the quality status of soft-bottom marine benthic communities. The aim of this study was investigating multivariate pattern of benthic invertebrate community and application of AMBI (AZTI's Marine Biotic Index) for determining quality of health status in Khuzestan coastal waters. Seasonal samplings were collected from eight creeks over one year study by Peterson grab sampler, beginning from October 2005. All creeks are characterized by muddy bottom. According to sensitivity to environmental stress, macrobenthic animals classified into 5 ecological groups. Among 28 identified faunal groups the most abundant groups were polychaets, molluscs, crustaceans and nematodes, respectively. Two way ANOVAs showed only seasonal significant differences in mean abundance (P<0.05) and Margalef richness index values (P<0.05), while all of studied creeks shows similar biological characters based on benthic communities. According to annual mean of AMBI value all creeks were in ecological group III with slightly polluted condition, except for Darvish that was in unpolluted category. In the present study, although the number of species initially increased, but due to appearance of dominant species such as Capitella sp. and nematods (as opportunist species), diversity values reduced. In general, according to AMBI and Biotic index values, the most creeks are classified into unpolluted and slightly polluted categories except for Zangy, Doragh and Patil in summer and also Zangy and Bihad in winter which was in moderate to heavily polluted category. Different types of exploitation, industrial and shipping activities in this area could result in unbalanced to polluted status in benthic animals (as the best indicators in sediment quality assessment) so these results can be acceptable.


Hadi Alavi S.M.,University of South Bohemia | Jorfi E.,South of Iran Aquaculture Research Center | Hatef A.,University of South Bohemia | Saheb Mortezavi S.A.,South of Iran Aquaculture Research Center
Aquaculture Research | Year: 2010

Spermatozoa concentration, ionic composition, osmolality, glucose and total protein contents of seminal plasma and sperm motility were determined in Barbus sharpeyi (Cyprinidae, Teleosotei). Spermatozoa concentration ranged from 9.77 to 20.20 × 10 9 spermatozoa mL -1. Osmolality (mOsmol kg -1) and ionic contents (mM L -1) of the seminal plasma were 274.5±9.0, 70.0±3.4 Na +, 28.8±0.9 K +, 101.7±3.1 Cl -, 0.9±0.1 Mg 2+ and 2.1±0.1 Ca 2+ respectively. Total protein and glucose were 5.3±0.2 g L -1 and 76.7±4.3 mM L -1 respectively. Sperm motility was initiated in a hypo-osmotic condition, composed of either an ionic (KCl or NaCl) or a non-ionic (sucrose) activation medium. Duration of sperm motility was very short: <2 min after activation in distilled water. Percentage of motile spermatozoa was significantly higher in an activation medium containing NaCl compared with that of distilled water. An activating medium containing NaCl or KCl higher than 150 mM or sucrose higher than 275 mM totally inhibited the activation of sperm motility. Immediately after sperm activation, wave(s) propagated along the flagellum, but waves were restricted to the proximal part of the flagellum (close to the head) at 1 min post activation. Studied characteristics in the present study were compared with those of other cyprinids for understanding inter-species differences. © 2010 The Authors. Aquaculture Research © 2010 Blackwell Publishing Ltd.


Jahani N.,South of Iran Aquaculture Research Center | Nabavi S.N.B.,Iran University of Science and Technology | Dehghan M.S.,South of Iran Aquaculture Research Center | Mortezaie S.R.S.,South of Iran Aquaculture Research Center | Fazeli N.,Iran University of Science and Technology
Iranian Journal of Fisheries Sciences | Year: 2012

The present work has been carried out to investigate the probable effects of fish cage culture on benthic communities as a pollution and stress indicator and to evaluate the biotic health condition using BOPA index, in Ghazale Creek (Khowre-Mussa - Persian Gulf). Monthly sampling from 4 stations was carried out from June 2007 to March 2008 (during nine months). Stations were selected from under the cage to 400 m distant (as control site) in Ghazale Creek. Three samples were taken at each station for macrobenthos and one for sediment grain size and total organic matter (TOM), using a 0.0125 m2 van veen grab. Also physical-chemical parameters sampling from three stations was done (during nine months). Stations were under cage station, 50 m and 400 m far from cages in Ghazale Creek.The percentage of total organic matter (TOM) in sediment ranged from 6.11 to 23.26 and the range of silty-clay percentage was from 4.76 to 97.47. The dominant macrobenthos groups were Polychaets (60.62%), Mulluska (19.67%), Crustacea (16.49%). Macrobenthic abundance, biomass and diversity index values in the under cage station were less than that in the control station. Comparing the results of BOPA with the guidelines shows that all stations had bad environmental conditions. The under cage station was more polluted than the control station. The range of physical-chemical parameters in water were: DO (6.5-11.43) ppm, BOD (1.5-10.9) ppm, Salinity (43-45.6) ppt, NO2- (0.006-0.29) ppb, NO3- (3.98-32.2) ppm, Turbidity (14-70) NTU temperature (11.8-32.5) °C.


Eskandari G.,South of Iran Aquaculture Research Center | Saghavi H.,South of Iran Aquaculture Research Center | Zabayeh Najafabadi M.,South of Iran Aquaculture Research Center | Dehghan Madiseh S.,South of Iran Aquaculture Research Center | Koochaknejad E.,South of Iran Aquaculture Research Center
Aquaculture Research | Year: 2013

More than 250 male and female yellowfin seabream (Acanthopagrus latus) were caught in the creeks near the Mahshar area in the north-west of Persian Gulf using fishhooks to study the effects of salinity on reproductive indices. The experiments were carried out using three salinity treatments (30 ± 1 g L-1, 35 ± 1 g L-1 and 40 ± 1 g L-1) with three replications. A total of six males and three females were randomly introduced to each tank. The survival rate of the broodstock was estimated at more than 90% at different salinity levels and the maximum rate was observed at 30 g L-1 of the experiment rate. The percentage of buoyant eggs was more than 90% at 40 g L-1 and it was significantly different from other treatments. The average number of eggs per female (312 914 ± 65 085), and the average number of eggs per kilogram of female (649 460 ± 173 574) at 40 g L-1 were more than those in the other treatments but no significant differences were observed (P ≤ 0.05). The average percentage of fertilized eggs (86.7%) and the average percentage of hatched larvae (67%) at 40 g L-1 treatment was more than those at 30 g L-1 and 35 g L-1 treatments but no significant differences were observed (P ≤ 0.05). At 40 g L-1 salinity, in all spawning cases, the released eggs were hatched. Overall, the present study has shown that better buoyant eggs of A. latus can be obtained at salinity 40 g L-1. On the other hand, the percentage of fertilized eggs and hatched larvae were not affected by salinity. © 2011 Blackwell Publishing Ltd.

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