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Kalbassi M.R.,Tarbiat Modares University | Lorestani R.,Tarbiat Modares University | Ghafle Maramazi J.,South Iran Aquaculture Research Center
Iranian Journal of Fisheries Sciences | Year: 2013

Regarding low fertilization rate of Barbus sharpeyi, one of the main Barbus species in south of Iran, saline activator solutions (SAS 1 and 2) were used for increasing of sperm activation and its sperm quality indices were assessed by using Image J software as a fish sperm adapted tools. SAS1 (45mM NaCl, 5mM KCl, 30mM Tris, 188±2.3 mOsmol/kg) and SAS2 (50mM NaCl, 30mM KCl, 30mM Tris, 189±1.7 mOsmol/kg) were added to a mixture of 12 male semen comparing with distilled and tap water and their effects on VCL, VSL, ALH, Linearity, %motility, spermatozoa path, % A, B and C spermatozoa type, fertilization, hatching and deformity rate were analyzed in triplicate. Results showed that SAS2 had the highest VCL (21.37±0.36 μ m/s), VSL (11.6±0.3 μ m/s) and motility percentage (85.75±1.56 %) among treatments. The highest A type spermatozoa percentage was recorded for SAS1 (47.16±3.15 %) and SAS2 (43.89±4.54%). The lowest C type spermatozoa percentage was recorded using SAS2 (14.24±1.56 %). Also, the highest fertilization rate was achieved from SAS2 (79.5±0.37%) and SAS1 (77.74±4%) respectively. Furthermore the lowest larval deformity rate (13.77±1.03%) was shown due to the use of SAS2. Final conclusion confirmed that SAS1 and SAS2 have positive and significant effects (P < 0.05) on extending the time length of sperms movement, VCL and VSL of B. sharpeyi which consequently resulted in better efficiency of propagation of this species in south of Iran. Source


Kalbassi M.R.,Tarbiat Modares University | Lorestani R.,Tarbiat Modares University | Marammazi J.G.,South Iran Aquaculture Research Center
Journal of Agricultural Science and Technology | Year: 2014

The main objective of this study was assessment of hormonal treatments effects on sperm quality improvement in Barbus sharpeyi. Results showed differences in effects of treatments with LHRHa2, LHRHa2 combined with a dopamine antagonist (MET), and CPE (Carp Pituitary Extract) on parameters of sperm volume, motility, spermatocrit, biochemical indices of seminal plasma and sperm efficiency on artificial propagation, incubation survival, and deformities. Biochemical analysis of seminal plasma showed that the majority of parameters were affected by different hormonal treatments. In conclusion, the highest sperm quality of Benni fish could be achieved by application of LHRHA2 (10 μg kg-1+MET), LHRHA2 (5 μg kg-1+MET) and LHRHA2 (5 μg kg-1) at 8, 12, and 16 hours PHS, respectively. Source


Gh E.,South Iran Aquaculture Research Center | Savari A.,Khorramshahr Marine Science and Technology University | Kochanian P.,Khorramshahr Marine Science and Technology University | Motlagh A T.,Iranian Fisheries Research Organization
Iranian Journal of Fisheries Sciences | Year: 2012

Estimates of age, growth parameters, length-weight relationship and length and age at first maturity of the otolithes ruber are required for fishery management. We used counting annuli on the section of sagittal otoliths to age O.ruber from the Northwest Persian Gulf in south of Iran. Estimated ages ranged from 0 to 6 years, and maximum frequency of fishes was observed in age-group 1. The values of growth parameters L8, k and to were calculated by von Bertalanffy model and the results were 67.57 (cm), 0.27 (year-1) and -0.43 respectively. Parameters b and an in length-weight relationship were calculated 3.19 and 0.005 respectively. Length and age at first maturity were estimated 28 cm and 1.55 year. Source


Maghsoudloo T.,Islamic Azad University at Tehran | Marammazi J.G.,South Iran Aquaculture Research Center | Kazemian M.,Islamic Azad University at Tehran | Paghe E.,South Iran Aquaculture Research Center
Iranian Journal of Fisheries Sciences | Year: 2012

The present study was carried out in order to establish an economical effective diet for the pacific white shrimp in the southern part conditions of Iran. Three dietary energy levels (E1=262, E2=312, E3=362 Kcal 100 g -1 diet) and 6 ratios of fish meal (FM): soybean meal (SBM) [(P1= 100% FM+ 0% SBM), (P2= 80% FM+ 20% SBM),(P3= 60% FM+ 40% SBM), (P4= 40% FM+ 60% SBM), (P5= 20% FM+ 80% SBM),(P6= 0% FM+ 100% SBM)], 18 experimental diets were prepared. Completely randomized design was used to assign 54 polyethylene 300 litre round tanks provided by aeration and was stocked by 19 juvenile shrimp as 3 replicates to each treatment. Shrimps average weight was about 0.77 g at the start. After 56 days culture period, maximum growth and nutritional performance were observed in the P6E1 and P5E1 treatments. In addition, the highest survival rate of the shrimps was observed in the P1E1, P1E2, P3E3 and P5E3 treatments. Results indicated that protein, fat, fiber and ash contents of carcase were significantly affected by the treatments (P<0.05). Results of the present study suggest the replacement possibility of at least 80% of dietary fishmeal by soybean meal in the diet of pacific white shrimp in the conditions of southern part of Iran. Source

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