Wang J.,Guangdong University of Technology |
Wang J.,South China State Key Laboratory Of Applied Microbiology Ministry Guangdong Prov Jointly Breeding Base |
Wu Q.-P.,South China State Key Laboratory Of Applied Microbiology Ministry Guangdong Prov Jointly Breeding Base |
Wu K.-G.,Guangdong University of Technology |
And 3 more authors.
Modern Food Science and Technology | Year: 2013
An efficient enterobacterial repetitive intergenic consensus sequence (ERIC-PCR) molecular typing method was established for Bacillus cereus. Based on the template of B. cereus CMCC 63303 genomic DNA, the ERIC-PCR amplification system in four levels of five factors (DNA template, Mg2+, dNTP, Taq DNA polymerase and primer) was optimized by orthogonal design L16(45).Then the effects of annealing temperature on the amplification system was discussed through single factor experiment and the optimal reaction condition were determined. Fifty B. cereus strains were typed using the optimal reaction system and cluster analysis. The ERIC-PCR results exhibited better discriminative results in molecular typing with discrimination index of 0.996. B. cereus strains were grouped into 47 types. The ERIC-PCR system was an efficient method for typing and tracking analyses. It was suitable for genetic diversity analysis of foodborne B. cereus strains.