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Liu B.,University of Sichuan | Wu J.-M.,University of Sichuan | Li J.,South China Center for Innovative Pharmaceuticals | Liu J.-J.,University of Sichuan | And 4 more authors.
Biochimie | Year: 2010

Polygonatum cyrtonema lectin (PCL), a mannose/sialic acid-binding lectin, has been reported to display remarkable anti-proliferative and apoptosis-inducing activities toward a variety of cancer cells; however, the precise molecular mechanisms by which PCL induces cancer cell death are still elusive. In the current study, we found that PCL could induce apoptosis and autophagy in murine fibrosarcoma L929 cells. Subsequently, we demonstrated that inhibition of Ras could promote L929 cell death, suggesting that Ras-Raf signaling pathway plays the key negative regulator in PCL-induced apoptosis. And, we showed that Ras-Raf signaling pathway was also involved in PCL-induced autophagy as the negative regulator. In addition, we found that class I phosphatidylinositol 3-kinase (PI3K)-Akt signaling pathway could play the negative regulator in PCL-induced apoptosis and autophagy. Taken together, these results demonstrate that PCL induces murine fibrosarcoma L929 cell apoptosis and autophagy via blocking Ras-Raf and PI3K-Akt signaling pathways. © 2010 Elsevier Masson SAS. All rights reserved. Source


Ji X.,South China Center for Innovative Pharmaceuticals | Feng Y.F.,Guangdong Pharmaceutical University | Rui W.,Guangdong Pharmaceutical University
Proceedings - 2012 International Conference on Biomedical Engineering and Biotechnology, iCBEB 2012 | Year: 2012

A rapid method for the chemical constituents analysis of Chinese herbal Rhizoma Anemarrhenae was developed by using ultra-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC/Q-TOF MS) in both positive and negative ESI mode. This method could provide good accuracy of molecular weight of each component found in the Rhizoma with relative deviation within 5 ppm. On the basis of the mass spectrometric fragmentation mechanisms, MS/MS data and relevant literatures, the constituents of Rhizoma Anemarrhenae, including 7 xanthones and 43 steroidal saponins, were identified, in which 17 new isomers and 9 components were discriminated in Rhizoma Anemarrhenae for the first time. Meanwhile, the fragmentation behaviors of individual components were discussed in details. © 2012 IEEE. Source


Zhang H.,Sun Yat Sen University | Zhu W.,Sun Yat Sen University | Su X.,Sun Yat Sen University | Wu S.,Sun Yat Sen University | And 10 more authors.
Journal of Neuro-Oncology | Year: 2012

Malignant glioma is the most devastating and aggressive tumor in brain, characterized by rapid proliferation and diffuse invasion. Chemotherapy and radiotherapy are the pivotal strategies after surgery; however, high drug resistance of malignant glioma and the blood-brain barrier usually render chemotherapy drugs ineffective. Here, we find that triptolide, a small molecule with high lipid solubility, is capable of inhibiting proliferation and invasion of malignant glioma cells effectively. In both investigated malignant glioma cell lines, triptolide repressed cell proliferation via inducing cell cycle arrest in G0/G1 phase, associated with downregulation of G0/G1 cell cycle regulators cyclin D1, CDK4, and CDK6 followed by reduced phosphorylation of retinoblastoma protein (Rb). In addition, triptolide induced morphological change of C6 cells through downregulation of protein expression of MAP-2 and inhibition of activities of GTPases Cdc42 and Rac1/2/3, thus significantly suppressing migratory and invasive capacity. Moreover, in an in vivo tumor model, triptolide delayed growth of malignant glioma xenografts. These findings suggest an important inhibitory action of triptolide on proliferation and invasion of malignant glioma, and encourage triptolide as a candidate for glioma therapy. © 2012 Springer Science+Business Media, LLC. Source


Zhang H.-L.,Guangdong Pharmaceutical University | Zhang H.-L.,South China Center for Innovative Pharmaceuticals | Tao Y.,Guangdong Pharmaceutical University | Guo J.,Guangdong Pharmaceutical University | And 2 more authors.
International Immunopharmacology | Year: 2011

The aim of this study was to investigate the hypolipidemic effects of chitosan nanoparticles(CTS-NP) preparations with ionotropic gelation, rotary evaporation, and spray-drying technique. Male SD (Sprague-Dawley) rats were separated into five groups, a normal diet group, a high fat emulsions group, a CTS control group and CTS-NP groups treated with two different doses of CTS-NP. The nanoparticles were spherical in shape and had a smooth surface. The size range of the nanoparticles was between 500 and 1000 nm. The apparent serum lipid and plasma viscosity in CTS-NP group was significantly lower than that in the CTS group, as well as the serum superoxide dismutate (SOD) levels was increased. Although no significant difference in adipose tissue was found among the groups, the rats fed on CTS-NP had lower relative liver weight and body weight when compared with those fed on normal diet. This study was the first report of the effects of CTS-NP in the hyperlipidemia rats, and suggests that the CTS-NP could be used for the treatment of hyperlipidemia. © 2010 Elsevier B.V. Source


Liu M.,Jinan University | Liu W.,Jinan University | Jiang J.,Jinan University | Cheng Q.,South China Center for Innovative Pharmaceuticals | Ren J.-L.,South China Center for Innovative Pharmaceuticals
Chinese Journal of New Drugs | Year: 2011

Objective: To synthesize the resveratrol analogs with more potent anti-oxidative activities. Methods: The A ring of resveratrol was substituted by TMP ring or 4-aminoquinoline ring. The DPPH radical scavenging abilities of the target molecules 9, 12 and intermediate 8, 11 were tested. Results and Conclusion: Two novel resveratrol analogues were synthesized, and their structures were confirmed by MS and 1H-NMR. Compoud 8 possesses more potent DPPH radical scavenging ability than resveratrol at low concentration and holds potent anti-oxidative activity. Source

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