Zeng F.,South China AgriculturalUniversity |
Zeng F.,Guangdong Provincial Key Laboratory of Agro Animal Genomics and Molecular Breeding |
Xie L.,Hainan Academy of Agricultural science |
Pang X.,South China AgriculturalUniversity |
And 6 more authors.
Poultry Science | Year: 2011
As a member of the G0/G1 switch genes,G0/G1 switch gene 2 (G0S2) is related to many regulatoryprocesses in the human and mouse. For example, itinteracts directly with adipose triglyceride lipase to activeits triglyceride hydrolysis activities. In this study,G0S2 gene cDNA of the chicken (522 bp), zebra finch(420 bp), sparrow (417 bp), pigeon (417 bp), and Bengalesefinch (416 bp) were cloned, and each of themwas encoded as a protein of 99 amino acids. The expressionof G0S2 mRNA was determined by real-timereverse-transcription PCR analysis in 20 tested tissuesof 21- and 91-d-old chickens. The highest mRNA levelwas found in abdominal fat and subcutaneous fat inboth stages. Considerable G0S2 mRNA was also observedin chicken heart and muscle tissues. Expressionof the chicken G0S2 gene varied at different stages andsexes. The abundance of G0S2 mRNA on d 21 was farhigher than that on d 91. The abundance in femalechickens was higher than that in males at both stages.In the coding region, we found 4 SNP, among whichonly G197A led to a change in the amino acids (Arg-66Gln); the rest were synonymous substitutions. Associationanalysis showed that both G102A and G255Awere significantly associated with head width (P <0.05) and were highly significantly associated with legmuscle color (P < 0.01). The G102A was significantlyassociated with shank diameter at 63 d (P < 0.05). TheSNP G197A was significantly associated with shank diameterat 49 d; CP content of leg muscle; total weightsof the heart, liver, gizzard, and glandular stomach; andsmall intestine length (P < 0.05). In conclusion, muchhigher G0S2 mRNA was detected in both male and femalechickens at 21 d of age than at 91 d of age, and 3SNP (G102A, G197, and G255A) were associated withchicken production traits. © 2011 Poultry Science Association Inc.