Sathish Kumar P.,Bharathidasan University |
Viswanathan M.B.G.,Bharathidasan University |
Venkatesan M.,Sourashtra College |
Balakrishna K.,Entomology Research Institute
Tumor Biology | Year: 2017
The triterpenoid, bauerenol, from Suregada angustifolia (Baill. ex Muell.-Arg.) Airy Shaw (Euphorbiaceae) was screened for anti-cancer property using hepatocellular carcinoma cell line, HepG2. Bauerenol exhibited growth inhibitory and apoptosis inducing potential against HepG2 cancer cells. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cytotoxic assay revealed that bauerenol treatment significantly reduced the growth of HepG2 cells in a time- and dose-dependent manner with 50% growth inhibitory concentration doses of 45 and 25 µg/mL at 24 and 48 h treatments, respectively. Bauerenol-induced cell death reflected apoptotic morphological features, that is, cell membrane blebbing, vacuolization, chromatin condensation, and nuclear fragmentation. In addition, bauerenol treatment diminished the mitochondrial membrane potential, by inducing the efflux of cytochrome c, downregulating the levels of anti-apoptotic Bcl-2 as well as upregulating the levels of pro-apoptotic Bax, and inducing caspase activation and poly (ADP-ribose) polymerase cleavage. Moreover, bauerenol treatment activates p38MAPK and inactivates the anti-apoptotic kinases Akt and ERK1/2 through the induction of reactive oxygen species. Furthermore, bauerenol-mediated S-phase arrest was associated with downregulation of cell cycle-rate-limiting factor (cyclin D1) and upregulation of cyclin-dependent kinase inhibitor p21 and tumor suppressor p53. Interestingly, pre-treatment of cells with reactive oxygen species inhibitor and p38 inhibitor significantly decreases bauerenol-induced cytotoxicity, Bax upregulation, and p38 activation. This study clearly states that bauerenol induces cell cycle arrest and apoptosis through the reactive oxygen species–dependent p38MAPK activation in HepG2 cancer cells. © 2017, © The Author(s) 2017.
Raman N.,VHNSN College |
Jeyamurugan R.,VHNSN College |
Subbulakshmi M.,VHNSN College |
Boominathan R.,VHNSN College |
Yuvarajan C.R.,Sourashtra College
Chemical Papers | Year: 2010
A novel series of Co(II), Ni(II), Cu(II), Zn(II), and VO(IV) complexes has been synthesized from the Schiff base derived from 4-[(3,4-dimethoxybenzylidene)amino]-1,5-dimethyl-2-phenyl-1,2-dihydropyrazol-3-one and 1,2-diaminobenzene. Structural features were determined by analytical and spectral techniques. Binding of synthesized complexes with calf thymus DNA (CT DNA) was studied by spectroscopic methods and viscosity measurements. Experimental results indicate that the complexes are able to form adducts with DNA and to distort the double helix by changing the base stacking. Lower DNA affinity of the VO(IV) complex is caused by the change of coordination geometry by the vanadyl ion resulting in a somewhat unfavorable configuration for the DNA binding. Oxidative DNA cleavage activities of the complexes were studied with supercoiled (SC) pUC19 DNA using gel electrophoresis; the mechanism studies revealed that the hydroxyl radical is likely to be the reactive species responsible for the cleavage of pUC19 DNA by the synthesized complexes. The in vitro antimicrobial screening effects of the investigated compounds were monitored by the disc diffusion method. The synthesized Schiff base complexes exhibit higher antimicrobial activity than the respective free Schiff base. © 2009 Institute of Chemistry, Slovak Academy of Sciences.
Rajasekar A.,National University of Singapore |
Anandkumar B.,Sourashtra College |
Maruthamuthu S.,CSIR - Central Electrochemical Research Institute |
Ting Y.-P.,National University of Singapore |
Rahman P.K.S.M.,University of Teesside
Applied Microbiology and Biotechnology | Year: 2010
Microbiologically influenced corrosion is a problem commonly encountered in facilities in the oil and gas industries. The present study describes bacterial enumeration and identification in diesel and naphtha pipelines located in the northwest and southwest region in India, using traditional cultivation technique and 16S rDNA gene sequencing. Phylogenetic analysis of 16S rRNA sequences of the isolates was carried out, and the samples obtained from the diesel and naphtha-transporting pipelines showed the occurrence of 11 bacterial species namely Serratia marcescens ACE2, Bacillus subtilis AR12, Bacillus cereus ACE4, Pseudomonas aeruginosa AI1, Klebsiella oxytoca ACP, Pseudomonas stutzeri AP2, Bacillus litoralis AN1, Bacillus sp., Bacillus pumilus AR2, Bacillus carboniphilus AR3, and Bacillus megaterium AR4. Sulfate-reducing bacteria were not detected in samples from both pipelines. The dominant bacterial species identified in the petroleum pipeline samples were B. cereus and S. marcescens in the diesel and naphtha pipelines, respectively. Therefore, several types of bacteria may be involved in biocorrosion arising from natural biofilms that develop in industrial facilities. In addition, localized (pitting) corrosion of the pipeline steel in the presence of the consortia was observed by scanning electron microscopy analysis. The potential role of each species in biofilm formation and steel corrosion is discussed. © 2009 Springer-Verlag.
Thirunarayanan G.,Annamalai University |
Sathiyendran V.,Sourashtra College |
Ravi K.,Proventus Life Science Private Ltd
Indian Journal of Chemistry - Section B Organic and Medicinal Chemistry | Year: 2011
A series of 6-substituted ω-bromo-2-naphthyl ketones and 6-substituted 2-naphthacyl esters have been synthesized by greener synthetic method using fly-ash:water catalyzed aqueous phase reaction. These ketones and esters have been characterized by their physical constants, GC-MS, IR and NMR spectral data. These compounds exist as two rotomers and the carbonyl frequencies of these rotomers have been assigned and correlated with Hammett substituent constants, F, R and Swain-Lupton's parameters. The antioxidant activities of the synthesized esters have been evaluated.
PubMed | Sourashtra College, Madurai Kamaraj University, Chemistry Research Center and Sethu Institute of Technology
Type: | Journal: Journal of photochemistry and photobiology. B, Biology | Year: 2016
Luotonin A (L), a novel natural cytotoxic and anti-inflammatory alkaloid, chelated with copper(II) to improve its cytotoxic effect against the cancer cells. The complexes [Cu(L)H
Emmanuel E.S.C.,St George College |
Ananthi T.,Sourashtra College |
Anandkumar B.,Indira Gandhi Center for Atomic Research |
Maruthamuthu S.,Control Electrochemical Research Institute
Journal of Biosciences | Year: 2012
In this study, Arthrobacter luteolus, isolated from rare earth environment of Chavara (Quilon district, Kerala, India), were found to produce catechol-type siderophores. The bacterial strain accumulated rare earth elements such as samarium and scandium. The siderophores may play a role in the accumulation of rare earth elements. Catecholate siderophore and low-molecular-weight organic acids were found to be present in experiments with Arthrobacter luteolus. The influence of siderophore on the accumulation of rare earth elements by bacteria has been extensively discussed. © 2011 Indian Academy of Sciences.
Rajasekaran M.,Sourashtra College |
Annaraj J.,Madurai Kamaraj University
Journal of Nanoscience and Nanotechnology | Year: 2014
Nanostructured phyto-drugs such as curcumin and quercetin were prepared by simple sonochemical method and studied for their bio-activities. FT-IR spectra indicate that the chemical structures of these nanostructured drugs are identical with their commercially available microcrystalline counterparts. Scanning Electron Microscopic (SEM) analysis reveals that the curcumin and quercetin, form as nanocube and nanoneedle like structures respectively. These nanocrystalline drugs have enhanced solubility in neutral aqueous medium at room temperature and thus exhibit better bioavailability than their commercially available microcrystalline form. Their DNA interactions and the observed binding constants were investigated through UV-vis spectral technique. Nanocurcumin, due to its small size, exhibits significantly high DNA binding constant (1.2×106 M-1). The voltammetric and CD spectral changes observed for the nanocurcumin reveal its deep penetration into the core of the DNA through the minor grooves, where it forms H-bonds with the floor nitrogen/oxygen atoms of the DNA bases. Copyright © 2014 American Scientific Publishers All rights reserved.
Arumugam G.,Madurai Kamaraj University |
Vijayakumar V.K.,Sourashtra College
Lecture Notes in Business Information Processing | Year: 2015
Association rule mining(ARM) techniques search for groups of frequently co-occurring items (i.e., frequent itemset) in a market-basket transaction database and convert these groups into business-oriented rules. The problem of ARM will gain momentum when it is attached with the time of transaction. High utility itemset mining is a research area of utility based data mining, aimed at finding itemsets that contribute most to the total utility. The association of time and utility on frequent itemsets gives a novel approach to efficiently capture the transactions for getting better predictions and planning for an enterprise. Previous research has focused mainly on how to obtain exhaustive lists of association rules. However, users often prefer a quick response to targeted queries. To accelerate the processing of such queries, a query-driven system called TD-FVAUFM (Time-Dependent Fast Value Added Utility Frequent Mining) is proposed in this paper. It performs data preprocessing steps on the given database and the resultant database is converted in the form of an itemset tree, a compact data structure suitable for query response. The proposed system is applied on a medical database containing patient’s records. It generates association rules that predict possible diseases with risk factor and frequency with respect to time. Experiments indicate that the targeted queries are answered in a time that is roughly linear in the number of transactions. © Springer International Publishing Switzerland 2015.
Rajesh M.,Sourashtra College
Journal of Pure and Applied Microbiology | Year: 2010
Aquatic systems are very sensitive to heavy metal pollutants and the gradual increase in the levels of such metals in aquatic environment, mainly due to anthropogenic sources, became a problem of primary concern. This is due to their persistence as they are not usually eliminated either by biodegradation or by chemical means, in contrast to most organic pollutants. Moreover, the decay of organic materials in aquatic systems together with detritus formed by natural weathering processes, provides a rich source of nutrients in both the bottom sediments and overlying water body. Microorganisms, microflora and algae are capable of incorporating and accumulating metal species into their living cells from various supply sources. Consequently, small fish become enriched with the accumulated substances. Eventually man, consuming the fish, inevitably suffers from the results of an enrichment taken place at each trophic level, where less is extracted than ingested (Forstner and Wittmann, 1983). In the present study, effect of Aluminium toxicity on carbohydrate, protein and lipid of Oreochromis mossambicus was seen. Our experiments demonstrate that increasing concentrations of aluminium decreases the carbohydrate, protein and lipid content of muscles and liver in the tilapia fish.
Poornima Devi G.,Sourashtra College
International Journal of Pharmacy and Technology | Year: 2016
Different aldehyde dehydrogenase (ALDH) knock-out mutants and transgenic Arabidopsis thaliana overexpressing ALDH genes are analysed in this study in order to establish the genotype of each independent plant and to elucidate the functional involvement of ALDH genes in plants response to abiotic stress. Approximately, hundred ALDH double knock-out plants are analysed by PCR amplification using two different pairs of primers for ALDH, out of 33 analysed plants three single knock-out plants for ALDH4, out of 42 analysed plants twelve single knock-out plants for ALDH5 and one double knock-out for ALDH3 and ALDH5 are found in total of 75 analysed plants. In addition, double overexpressing plants for ALDH3I1 and ALDH7B4 genes, are identified in the same plant. Out of 24 plants analysed, 6 plants are detected to have both ALDH transgenes. Further, the accumulation of ALDH3I1 and ALDH7B4 proteins are detected in the double overexpressing plants using respective ALDH antibody. The follow-up work is to carry out the physiological and biochemical characterization of these plants under different abiotic stress conditions. © 2016, International Journal of Pharmacy and Technology. All rights reserved.