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Bhattacharya T.,Institute of Science and Technology for Advanced Studies and Research | Tiwari K.K.,Sophisticated Instrumentation Center for Applied Research and Testing
Journal of Environmental Biology | Year: 2010

The present investigation was undertaken to assess the biodegradation of phenol by native bacteria strains isolated from coke oven processing wastewater. The strains were designated ESDSPB1, ESDSPB2 and ESDSPB3 and examined for colony morphology Gram stain characters and biochemical tests. Phenol degrading performance of all the strains was evaluated initially One of the strains namely ESDSPB2 was found to be highly effective for the removal of phenol, which was used as sole carbon and energy source. From an initial concentration of 200 mg ť it degraded to 79.84±1.23mg I'. In turn the effect of temperature (20 to 45°C), pH (5-10) and glucose concentration (0,0.25 and 0.5%) on the rate of phenol degradation by that particular strain was investigated. Observations revealed that the rate of phenol biodégradation was significantly affected by pH, temperature of incubation and glucose concentration. The optimal conditions for phenol removal were found to be pH of 7 (84.63% removal), temperature, 30°C (76.69% removal) and 0.25% supplemented glucose level (97.88% removal). The main significance of the study is the utilization of native bacterial strains from the waste water itself having potential of bioremediation. © Triveni Enterprises, Lucknow (India).


Dwivedi S.,CSIR - Central Electrochemical Research Institute | Mishra A.,CSIR - Central Electrochemical Research Institute | Kumar A.,CSIR - Central Electrochemical Research Institute | Tripathi P.,CSIR - Central Electrochemical Research Institute | And 6 more authors.
Clean Technologies and Environmental Policy | Year: 2012

During the present study, two species of Portulaca, i.e., P. tuberosa and P. oleracea were collected from two field sites in Vadodara, Gujarat, India; one irrigated with industrial effluent and other with tube well water, and were analyzed for heavy metal accumulation in different plant parts viz., roots, stem, leaves, and flowers. Plants collected from effluent irrigated areas showed high accumulation of all the investigated heavy metals in all plant parts with the maximum being in roots and the least in flowers. Interestingly, both species of Portulaca demonstrated hyperaccumulation of multiple elements viz., Cu, Ni, Hg, and Pb. The total shoot concentrations (μg g -1 dw) of Cu, Ni, Hg, and Pb in P. tuberosa were 1538, 1191, 789, and 2744, respectively, while in P. oleracea, these were 1940, 1542, 534, and 2312, respectively. Besides this, selective hyperaccumulation of Se (2,327 μg g -1 dw) and Al (1,164 μg g -1 dw) was shown by P. tuberosa and P. oleracea, respectively. Total shoot concentrations (μg g -1 dw) of Mo were about 399 and 668 in P. tuberosa and P. oleracea, respectively. Overall, P. oleracea accumulated higher amounts of multiple metals from industrial effluent contaminated site, hence appears to be a suitable candidate for phytoremediation purposes of metal contaminated areas. © 2011 Springer-Verlag.


Tiwari K.K.,Sophisticated Instrumentation Center for Applied Research and Testing | Singh N.K.,National Botanical Research Institute | Patel M.P.,Sophisticated Instrumentation Center for Applied Research and Testing | Tiwari M.R.,Sophisticated Instrumentation Center for Applied Research and Testing | Rai U.N.,National Botanical Research Institute
Ecotoxicology and Environmental Safety | Year: 2011

The present investigation was carried out to evaluate metals concentration in ten vegetable crops growing in mixed industrial effluent irrigated agricultural field near Vadodara, Gujarat, India. Differential accumulation and translocation of various metals in selected vegetables plant species was observed. A higher concentration of metals were found in order of Fe>Mn>Zn>Cd>Cu>Pb>Cr>As in soil irrigated with industrial effluent than soil irrigated with tube well water; however, the concentration of As, Cr and Pb found below detection limit in tube well water irrigated soil. Metal accumulation in root and top of vegetables varied significantly both in relations to metal concentration in the soil and the plant genotype. Among ten vegetable species studied five vegetable species, i.e. Spinach, Radish, Tomato, Chili and Cabbage growing in mixed industrial effluent irrigated agricultural field showed high accumulation and translocation of toxic metals (As, Cd, Cr, Pb and Ni) in their edible parts, thus, their cultivation are unsafe with respect to possible transfer in food chain and health hazards. However, it is suggested that vegetable crops restricting toxic metal in non-edible port may be recommended for cultivation in such metal contaminated agricultural field. © 2011 Elsevier Inc.


Mishra V.,Institute of Science and Technology for Advanced Studies and Research ISTAR | Mishra V.,Sophisticated Instrumentation Center for Applied Research and Testing | Mohanty I.,Institute of Science and Technology for Advanced Studies and Research ISTAR | Patel M.R.,Institute of Science and Technology for Advanced Studies and Research ISTAR | Patel K.I.,Institute of Science and Technology for Advanced Studies and Research ISTAR
International Journal of Polymer Analysis and Characterization | Year: 2015

A series of novel waterborne UV-curable polyurethane dispersions were synthesized from castor oil, IPDI, DMPA, and HEMA by the acetone process. The prepared UV-PUD was mixed with three different monomers and characterized for the parameters relevant to the study by physical, spectroscopic, and chemical methods. The effect of monomer type on the physicochemical and thermal properties of UV-PUDs revealed that the number of unsaturated groups present in the monomer had a pronounced effect on physicochemical, mechanical, and thermal properties. The combination of renewable resource and environmentally compliant technology give these UV-PUDs potential scope in coating applications. © 2015, Copyright © Taylor & Francis Group, LLC.


Tiwari K.K.,Sophisticated Instrumentation Center for Applied Research and Testing | Singh N.K.,CSIR - Central Electrochemical Research Institute | Rai U.N.,CSIR - Central Electrochemical Research Institute
Bulletin of Environmental Contamination and Toxicology | Year: 2013

In the present investigation, chromium (VI) induced toxicity on metabolic activity and translocations of nutrients in radish were evaluated under controlled glass house conditions. Chromium was found to induce toxicity and significantly affect plant growth and metabolic activity. Excess of chromium (0.4 mM) caused a decrease in the concentration of iron in leaves (from 134.3 to 71.9 μg g-1 dw) and significant translocation of sulphur, phosphorus and zinc. Translocation of manganese, copper and boron were less affected from root to stem. After 15 days of Cr exposure, maximum accumulation of Cr was found in roots (327.6 μg g-1 dw) followed by stems (186.8 μg g-1 dw) and leaves (116.7 μg g-1 dw) at 0.4 mM Cr concentration. Therefore, Cr may affect negatively not only production, but also the nutritive quality of the radish; likewise, higher Cr content may cause health hazards for humans. © 2013 Springer Science+Business Media New York.


Chauhan G.R.,Sophisticated Instrumentation Center for Applied Research and Testing | Chauhan G.R.,P.A. College | Kharadi G.J.,Government Science College | Patel K.D.,Sophisticated Instrumentation Center for Applied Research and Testing | Patel K.D.,P.A. College
Oriental Journal of Chemistry | Year: 2010

The mixed-ligand complexes of various metal(ll) with 5-chloro-7-iodo-8- hydroxyquinoline (Clioquinol) and 5-(methoxymethyl-8-quinolinol) (MMQ) were prepared. The structure of mixed-ligand complexes was investigated using spectral, physicochemical, elemental analyses and thermal studies. Magnetic moment and reflectance spectral studies reveal that an octahedral geometry has been assigned to all the prepared complexes. The ligands, metal salts, complexes, control and standard drug were tested for their in-vitro antifungal.The metal complexes exhibit good activity against fungal strains compared with parental compounds and moderate compared with the standard drug (Clioquinol).


PubMed | Sophisticated Instrumentation Center for Applied Research and Testing
Type: Journal Article | Journal: Journal of environmental biology | Year: 2010

Due to widespread industrial use, chromium (Cr) is considered a hazardous environmental pollutant. It is known to inhibit plant growth and development. The present study provides the evidence of the phytotoxicity of this metal on the pea (Pisum sativum L. cv Azad) plants. The plants of pea (Pisum sativum L.) were grown in refined sand under different concentrations i.e. 0.05, 0.1, 0.2, 0.3 and 0.4 mM of Cr (VI) in order to study the effect on growth and yield, photosynthetic pigments, relative water content, non-reducing sugar and protein with activity of certain enzymes like catalase, peroxidase, starch phosphorylase and ribonuclease. The analysis of the results showed that photosynthetic pigments (68.68%), relative water contents (62.77%), non-reducing sugar (66.66%) and protein (81.57%) were decrease along with reduction in plant height (52.69%) and leaf area (50.81%) of the pea plants. However, in response to various concentration of Cr exposed plants showed significant induction of reducing and total sugars with enzymes like catalase, starch phosphorylase and ribonuclease. The translocation of Cr in various part of pea plant have been found in order of root> stem> leaves>seeds which ranged between 34.8 to 217.3 mg g(-1) d.wt. (dry weight) in roots, 6.5 to 173.13 mg g(-1) d.wt. in shoot, 4.2 to 74.43 mg g(-1) d.wt. in leaves and 0.94 to 8.64 mg g(-1) d.wt. in seeds, that is also reflected by the transfer factor of Cr from refined sand to tested species.


PubMed | Sophisticated Instrumentation Center for Applied Research and Testing
Type: Journal Article | Journal: Journal of AOAC International | Year: 2010

Many polyherbal oil formulations in traditional systems of medicine contain aconite root. This paper reports the development and validation of a simple, rapid, and sensitive HPTLC method for identification and quantification of aconitine from polyherbal oil formulations. Chromatography of methanolic extract of these formulations was performed on silica gel 60F254 aluminum-backed HPTLC plates with a 0.2 mm layer thickness. The plates were developed up to 85 mm with the binary mobile phase ethyl acetate-ethanol (7.5 + 2.5, v/v) at 22 +/- 2 degrees C with 20 min of chamber saturation. The system produced a compact band of the marker aconitine at an R(f) value of 0.33 that was quantified at its maximum absorbance of 238 nm. The LOD and LOQ values were found to be 20 and 70 ng/band, respectively. The linearity with respect to peak area was in the range of 300 to 1800 ng/band with an r of 0.9991. Polyherbal oil formulations were analyzed with reasonable accuracy, and no matrix interference was observed. The developed HPTLC method is accurate, precise, and cost-effective, and can be used for marker-based QA of polyherbal oil formulations containing Aconitum chasmanthum as one of the active ingredients.


PubMed | Sophisticated Instrumentation Center for Applied Research and Testing
Type: Journal Article | Journal: Bulletin of environmental contamination and toxicology | Year: 2013

In the present investigation, chromium (VI) induced toxicity on metabolic activity and translocations of nutrients in radish were evaluated under controlled glass house conditions. Chromium was found to induce toxicity and significantly affect plant growth and metabolic activity. Excess of chromium (0.4 mM) caused a decrease in the concentration of iron in leaves (from 134.3 to 71.9 g g(-1) dw) and significant translocation of sulphur, phosphorus and zinc. Translocation of manganese, copper and boron were less affected from root to stem. After 15 days of Cr exposure, maximum accumulation of Cr was found in roots (327.6 g g(-1) dw) followed by stems (186.8 g g(-1) dw) and leaves (116.7 g g(-1) dw) at 0.4 mM Cr concentration. Therefore, Cr may affect negatively not only production, but also the nutritive quality of the radish; likewise, higher Cr content may cause health hazards for humans.

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