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Lee J.-W.,Chung - Ang University | Son J.,Chung - Ang University | Yoo K.-M.,Soong Eui Womens College | Lo Y.M.,University of Maryland University College | Moon B.,Chung - Ang University
RSC Advances | Year: 2014

Gold@platinum (Au@Pt) nanostructures exhibit desirable catalytic abilities and unique optical properties, which are not seen in single-component metal nanoparticles (NPs). To date, however, there is only limited information available in the literature on the integrated approach to investigate the antioxidant activities of Au@Pt NPs. The aim of the present study, was to evaluate the antioxidant activities of hybrid nanorods, i.e., Au@Pt NPs. Specifically, the free radical scavenging effect, hydroxyl radical scavenging activity, and superoxide dismutase (SOD)-like activity of Au@Pt NPs were examined. Furthermore, the in vitro antioxidant ability of Au@Pt NPs was assessed by measuring the cell viability of V79-4 (Chinese hamster lung fibroblasts), PC-3 (human prostate cancer), and MCF-7 (human breast cancer) cells under oxidative stress induced by hydrogen peroxide (H2O 2). The results showed that Au@Pt NPs exhibited high DPPH radical scavenging activity, non-site specific hydroxyl radical scavenging activity, linoleic acid peroxidation inhibitory ability by TBA tests in a low Au@Pt NP concentration range. Furthermore, in vitro experiments revealed that Au@Pt NPs showed cellular protection against oxidative stress induced by H 2O2. © 2014 the Partner Organisations.

Kim H.J.,Seoul National University | Yoo K.M.,Soong Eui Womens College | Lee S.,Seoul National University | Kim K.-T.,Korea Food Research Institute | Hwang I.K.,Seoul National University
Korean Journal of Food Science and Technology | Year: 2013

The aim of our study was to investigate the chemical composition of the Asian ginseng seed (Panax ginseng C.A. Meyer) and the American ginseng seed (Panax quinquifolium L.) grown in Korea (3 years, KGS3; 4 years, KGS4), China (4 years, CGS4), and USA (4 years, AGS4). AGS had the heaviest 100-seed weight (4.21±0.31 g). The approximate compositions of the ginseng seeds were 13.66-17.00% crude protein, 2.21-8.65% crude ash, 19.06-24.06% crude lipid, and 43.21-47.49% crude fiber. The mineral contents of the ginseng seeds were greater in order of K>P>Ca>Mg>Fe>Na>Zn >Cu. The unsaturated fatty acid content was 96.71-96.94%, and the major fatty acids oleic acid and linoleic acid were present. Total sugar content was 15.00-26.17 mg glucose/g. The acidic polysaccharide content was 0.56-0.80 mg β-Dgalacturonic acid/g. These results showed the differences in the physicochemical characteristics of ginseng seeds with respect to cultivation location, cultivation year, and species.

Kim J.-E.,Seoul National University | Lee S.,Seoul National University | Yoo K.-M.,Soong Eui Womens College | Lee K.-H.,Dongnam Health College | And 3 more authors.
Journal of the Korean Society of Food Science and Nutrition | Year: 2014

This study aimed to determine the quality characteristics of ginseng seed oil as well as evaluate the efficacy of ginseng seed oil as a food resource. Ginseng seed oil was obtained by different extraction methods; from solvent extraction oil, supercritical fluid extraction oil, and screw pressed extraction oil. Total unsaturated fatty acids were present at 97.72~97.92%. Oleic acid (80.13~81.16%) was the highest, followed by linoleic acid (14.98~15.69%). The total phenol content (mg gallic acid equivalent/100 g oil) was higher in screw pressed extraction oil (56.32±1.47) compared to others. γ-Tocopherol was only present in ginseng seed oil and screw pressed extraction oil showed the highest levels of γ-tocopherol (5.95±0.25 mg/100 g oil) among the tested samples. Screw pressed extraction oil showed the greatest oxidative stability with an induction time of 16.58 hours. Acid values and peroxide values of ginseng seed oil increased with increasing storage period. The total phenol and γ-tocopherol contents were higher in screw pressed extraction oil than in other ginseng seed oils, which suggests that screw pressed extraction oil has the greatest oxidative stability.

Huang Y.,Seoul National University | So Y.-J.,Seoul National University | Hwang J.R.,Seoul National University | Yoo K.-M.,Soong Eui Womens College | And 3 more authors.
Korean Journal of Food Science and Technology | Year: 2014

This study aimed to investigate the activities of phytochemical and bioactive components from 24 new varieties of red pepper obtained by crossbreeding different Korean red pepper lines. The ASTA (American Spice Trade Association) color values ranged from 116.69 to 249.83 and the total carotenoid content ranged from 3.19 to 6.11 mg/g dried red pepper. The free sugar content ranged from 43.41 to 199.54 mg/g, and the fructose content was found to be higher than the glucose content. The ascorbic acid, total polyphenol, and flavonoid contents were found to be 8.08-11.53 mg/g, 7.93- 14.26 mg gallic acid/g, and 4.24-10.07 mg quercetin/g dried pepper, respectively. Overall, the total polyphenol contents of the new varieties were found to be higher than the average content of Korean red peppers. The total capsaicinoid content in the new varieties varied widely from 0.42 to 237.87 mg/100 g dried pepper. The pepper-methanol extract antioxidant activities of 'variety 2, 4, 8, 9' were higher than others. A high correlation was observed between the antioxidant activities and the polyphenol contents (r=0.804, r=0.701). © The Korean Society of Food Science and Technology.

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