Time filter

Source Type

Pomacle, France

Hamzaoui M.,CNRS Institute of Molecular Chemistry - Reims | Renault J.-H.,CNRS Institute of Molecular Chemistry - Reims | Nuzillard J.-M.,CNRS Institute of Molecular Chemistry - Reims | Reynaud R.,Soliance | Hubert J.,CNRS Institute of Molecular Chemistry - Reims
Phytochemical Analysis | Year: 2013

Introduction Tree bark represents an interesting source of bioactive molecules for the discovery of new pharmaceutical agents. However, the detailed screening of secondary metabolites in crude bark extracts is often hampered by the presence of tannins, which are difficult to separate from other plant constituents. Objective In the present study, a new centrifugal partition extraction (CPE) method was developed in order to fractionate a crude bark extract of Anogeissus leiocarpus Guill. & Perr. (Combretaceae). Methods A three-phase solvent system composed of n-heptane, methyl tert-butyl ether, acetonitrile and water was optimised for the stepwise elution at 20 mL/min of different phytochemical classes according to their hydrophobicity. Onedimensional and two-dimensional NMR analyses of the simplified fractions were then performed in order to characterise potentially interesting metabolites. Results In one step, 5 g of the initial crude extract were efficiently fractionated to yield highly simplified fractions that contained triterpenes, ellagic acid derivatives, flavonoids and phenolic compounds. All undesired compounds, that is, the highly abundant water-soluble tannins (78.8%), were totally removed and each run was rapidly achieved in 90 min on a the multi-gram scale and with low solvent volumes. Conclusion Centrifugal partition extraction in the elution mode using a three-phase solvent system can thus be proposed as an efficient and cost-effective alternative for a rapid fractionation of crude bark extracts and for an effective screening of potentially active secondary metabolites. Copyright © 2013 John Wiley & Sons, Ltd.

Hamzaoui M.,CNRS Institute of Molecular Chemistry - Reims | Renault J.-H.,CNRS Institute of Molecular Chemistry - Reims | Reynaud R.,Soliance | Hubert J.,CNRS Institute of Molecular Chemistry - Reims
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | Year: 2013

Centrifugal partition extraction (CPE) was developed for the first time in the pH-zone-refining mode to fractionate a crude bark extract of the African tree Anogeissus leiocarpus Guill. & Perr. (Combretaceae). The fractionation process was performed at a flow rate of 20. mL/min using a biphasic solvent system composed of methyl tert-butyl ether/acetonitrile/water (4:1:5, v/v/v) in the ascending mode. Sodium hydroxide (40. mM) and trifluoroacetic acid (30. mM) were used as retainer and displacer agents, respectively. In a single run of 67. min, 3. g of the initial crude extract were successfully separated into fractions selectively enriched in ionizable triterpenes, ellagic acid derivatives and flavonoids. The antioxidant potential of the initial crude extract, isolated compounds and fraction pools was also evaluated by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH.) stable free radical scavenging assay, providing an interesting view about the effect of the degree of substitution of ellagic acid derivatives on their radical scavenging activity. This study will demonstrate that centrifugal partition extraction used in the pH-zone-refining mode can be proposed as an efficient strategy for the rapid screening of natural phenolic compounds. © 2013 Elsevier B.V.

Agency: Cordis | Branch: FP7 | Program: MC-IAPP | Phase: FP7-PEOPLE-2013-IAPP | Award Amount: 2.19M | Year: 2013

MICROSMETICS aims to discover and carry to the stage of development innovative products in the area of cosmeceuticals originating from global biodiversity using emerging and state of the art technologies in the field of biotechnology, natural products chemistry and applied microbiology. These objectives will be implemented through an extended and balanced scheme of researchers exchanges and recruitments, in both directions and via a mutual scientific project developed on the needs and interests of both Industrial and Academic sectors, exploiting the existing complimentary expertise. More specifically, MICROSMETICS scientific concept involves the discovery of novel natural products originating from global microbial biodiversity. Already existing culture collections will be exploited incorporating modern high throughput platforms (in silico & in vitro) for the rational and targeted selection of the most promising strains. Advanced analytical approaches and techniques will be applied for the efficient, accelerated and advantageous isolation and identification of natural constituents as well as the quality assessment of the lead products. A broad spectrum of bioassays and novel analytical approaches will be incorporated for the evaluation of anti-ageing, more specifically anti-oxidant, skin-protecting, and skin-whitening activity of all derived products. Attention will be given to the selection and optimisation of fermentation technologies used for the production of final lead products to ensure sustainability. Within this frame, core scientific knowledge and lead compounds for further development are expected to be produced creating valuable synergies. Expertise will be transferred by means of the seconded researchers training in environments with different dynamics and orientation. MICROSMETICS aspires to comprise a successful model of long-lasting collaboration between Industry and Academia for sustainable exploitation of existing know-how and produced knowledge.

Soliance | Date: 2016-03-15

Chemical products for use in the manufacture of cosmetics, namely, raw materials as additive or active ingredients for cosmetic purposes, for anti-aging, moisturizing, skin complexion, lightening, protection, soothing, functional agent and firming.

Soliance | Date: 2013-05-24

Composition containing at least one substance that is not water-soluble and is solubilised in a sophorolipid, and at least water, and use thereof for cleaning hard surfaces and laundry, for killing plants, for combating insect pests, for combating fungus and mould proliferation, for combating gastropods, for combating moss, algae and lichen proliferation, for the cosmetics industry and for skin and hair care.

Discover hidden collaborations