Toulouges, France
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Roger B.,Societe Phytotagante | Jeannot V.,Societe Phytotagante | Fernandez X.,CNRS Institute of Chemistry | Cerantola S.,Service Communications de RMN RPE | Chahboun J.,Societe Phytotagante
Phytochemical Analysis | Year: 2012

Introduction Iris resinoid obtained from Iris germanica or Iris pallida rhizomes is widely used in the perfume industry but its chemical composition has not yet been reported. Nevertheless, very active compounds have been identified in iris rhizomes including iridals and isoflavones. Objective In this first study concerning iris resinoid composition, flavonoids were qualitatively and quantitatively investigated in I. germanica and I. pallida resinoids. Methodology Resinoids were first fractionated by reverse-phase flash chromatography in order to obtain fractions containing all isoflavones. These fractions were analysed by HPLC-DAD (diode array detector) and the fractions containing isoflavones were analysed by HPLC-QTOF (quadrupole time of flight)-MS. Then, the main isoflavones were isolated and identified by NMR and high-resolution mass spectroscopy (HRMS). Finally, total and individual isoflavones were quantified by HPLC-DAD at 265 nm using an external calibration method with irigenin as the external standard. Results Eight isoflavones were identified in both resinoids (irigenin, iristectorigenin A, nigricin, nigricanin, irisflorentin, iriskumaonin methyl ether, irilone, iriflogenin), one isoflavone only was identified in I. germanica resinoid (irisolidone), whereas one isoflavone (8-hydroxyirigenin), one isoflavanone (2,3-dihydroirigenin) and one benzophenone (2,6,4'-trihydroxy-4-methoxybenzophenone) only were identified in I. pallida resinoid. Isoflavones were quantified in I. germanica and I. pallida resinoids at 180 ± 1.6 mg/g and 120 ± 3.3 mg/g respectively. Conclusion The study shows that I. germanica and I. pallida resinoids are rich in flavonoids and that these two Iris species can be distinguished by simply analysing the polyphenol fraction. Copyright © 2011 John Wiley & Sons, Ltd.


Roger B.,Societe Phytotagante | Burger P.,CNRS Institute of Chemistry | Baret P.,University of Reunion Island | Chahboun J.,Societe Phytotagante | And 3 more authors.
Journal of Essential Oil Research | Year: 2016

Orange blossom water (Citrus aurantium L. ssp aurantium) is a hydrosol widely used in cosmetics, medicine and in the food industry. When exposed to sunlight for a few days, the hydrosol turns orange and a precipitate appears afterwards. Despite the economic importance of the orange blossom water, this phenomenon has never been studied and the chemical changes taking place have never been elucidated. This study reports on the comparative analysis of sunlight exposed and non-exposed hydrosols by UV-Visible spectroscopy, GC and HPLC. The formation of several indole derivatives (isatin, oxindole, indole-3-carboxaldehyde, 3,3'-diindolylmethane, turbomycin A) induced by the exposition of orange blossom water to sunlight was observed. Two of these derivatives have already been identified as biologically active compounds: 3,3'-diindolylmethane displays an antiproliferative activity against various human cancer cell lines and turbomycin A presents a broad-range antibiotic activity. The later one was identified in this study as the compound responsible for the orange coloration of sunlight-exposed orange blossom water. © 2015 Taylor & Francis.


Roger B.,CNRS Institute of Chemistry | Roger B.,Societe PHYTOTAGANTE | Fernandez X.,CNRS Institute of Chemistry | Jeannot V.,Societe PHYTOTAGANTE | Chahboun J.,Societe PHYTOTAGANTE
Phytochemical Analysis | Year: 2010

Introduction - The essential oil obtained from iris rhizomes is one of the most precious raw materials for the perfume industry. Its fragrance is due to irones that are gradually formed by oxidative degradation of iridals during rhizome ageing. Objective - The development of an alternative method allowing irone quantification in iris rhizomes using HS-SPME-GC. Methodology - The development of the method using HS-SPME-GC was achieved using the results obtained from a conventional method, i.e. a solid-liquid extraction (SLE) followed by irone quantification by CG. Results - Among several calibration methods tested, internal calibration gave the best results and was the least sensitive to the matrix effect. The proposed method using HS-SPME-GC is as accurate and reproducible as the conventional one using SLE. These two methods were used to monitor and compare irone concentrations in iris rhizomes that had been stored for 6 months to 9 years. Conclusion - Irone quantification in iris rhizome can be achieved using HS-SPME-GC. This method can thus be used for the quality control of the iris rhizomes. It offers the advantage of combining extraction and analysis with an automated device and thus allows a large number of rhizome batches to be analysed and compared in a limited amount of time. © 2010 John Wiley & Sons, Ltd.


PubMed | Societe Phytotagante
Type: Journal Article | Journal: Phytochemical analysis : PCA | Year: 2012

Iris resinoid obtained from Iris germanica or Iris pallida rhizomes is widely used in the perfume industry but its chemical composition has not yet been reported. Nevertheless, very active compounds have been identified in iris rhizomes including iridals and isoflavones.In this first study concerning iris resinoid composition, flavonoids were qualitatively and quantitatively investigated in I. germanica and I. pallida resinoids.Resinoids were first fractionated by reverse-phase flash chromatography in order to obtain fractions containing all isoflavones. These fractions were analysed by HPLC-DAD (diode array detector) and the fractions containing isoflavones were analysed by HPLC-QTOF (quadrupole time of flight)-MS. Then, the main isoflavones were isolated and identified by NMR and high-resolution mass spectroscopy (HRMS). Finally, total and individual isoflavones were quantified by HPLC-DAD at 265nm using an external calibration method with irigenin as the external standard.Eight isoflavones were identified in both resinoids (irigenin, iristectorigenin A, nigricin, nigricanin, irisflorentin, iriskumaonin methyl ether, irilone, iriflogenin), one isoflavone only was identified in I. germanica resinoid (irisolidone), whereas one isoflavone (8-hydroxyirigenin), one isoflavanone (2,3-dihydroirigenin) and one benzophenone (2,6,4-trihydroxy-4-methoxybenzophenone) only were identified in I. pallida resinoid. Isoflavones were quantified in I. germanica and I. pallida resinoids at 1801.6mg/g and 1203.3mg/g respectively.The study shows that I. germanica and I. pallida resinoids are rich in flavonoids and that these two Iris species can be distinguished by simply analysing the polyphenol fraction.

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