SN Vanitha Mahavidyalaya Pharmacy College for Women

Hyderabad, India

SN Vanitha Mahavidyalaya Pharmacy College for Women

Hyderabad, India
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Rama Rao K.,Sree Dattha Institute of Pharmacy | Rama Rao K.,Acharya Nagarjuna University | Prakash K.,SN Vanitha Mahavidyalaya Pharmacy College for Women | Prasad C.V.V.,Sree Dattha Institute of Pharmacy
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2011

Rapid and simple bioanalytical high performance liquid chromatographic (HPLC) method for the determination of Ranitidine using Nizatidine as an internal standard was developed and validated as per regulatory requirements. Sample preparation was accomplished through solid phase extraction and chromatographic separation on a reverse phase column. The mobile phase consists of mixture of phosphate buffer and methanol at a flow rate of 1 ml/min. The wavelength used for the detection of ranitidine was 315nm with a total run time of 8 minutes. The retention times of ranitidine and nizatidine were found to be 6 and 4.5 minutes respectively. The method was developed and tested for the linearity range of 10 ng/ml to 1000 ng/ml. The method was validated for accuracy, precision, linearity, recovery and stability in compliance to international regulatory guidelines.

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