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Gheorghe C.G.,SN Pasteur SA Filipesti subsidiary | Pantea O.,Petroleum Gas University of Ploiesti | Matei V.,Petroleum Gas University of Ploiesti | Bombos D.,Petroleum Gas University of Ploiesti | Borcea A.-F.,Petroleum Gas University of Ploiesti
Revista de Chimie | Year: 2011

The tubes in which the tests were made contained a 5 mL bacterial suspension 1 Mc Fariand diluted in sterile saline. Over the content of each tube (except control tubes) were placed 200 μI of three contaminators (a tube corresponding to a contaminator). The concentration of the contaminated tubes was 40 μL /mL Incubation between fotometerings was made at 30°C. After each fotometering, decimal dilutions were made in sterile saline. It was followed the viability of bacteria in environments with hydrocarbons, compared with the viability of bacteria without contaminators. From these dilutions, inoculations were made in Petri dishes containing agar solid medium. The surviving bacterial colonies were counted after 48 h of incubation. During incubation, the tested medium did not contain nutrients to promote the development of cell mass (bacterial suspensions were diluted in sterile saline). Thus, hydrocarbons were used as the sole carbon source to observe the viability of various bacteria in similar environments compared with their viability in environments without contaminators and nutrients (control samples).


Gheorghe C.G.,SN Pasteur SA FILIPESTI subsidiary | Pantea O.,Petroleum Gas University of Ploiesti | Matei V.,Petroleum Gas University of Ploiesti | Bombos D.,Petroleum Gas University of Ploiesti | Borcea A.-F.,Petroleum Gas University of Ploiesti
Revista de Chimie | Year: 2011

Studies were made on the water from the oil sludge basin where petroleum residues were stored as well as sludge from the treatment plant of wastewater from refinery. The aim of the experiment program is biodegradation of petroleum waste deposit and removal of contaminants, turning it in a state that allows chemical, physical and biological treatment. Contaminants of these samples are damaging the environment and there is important to find solutions in order to eliminate these contaminants. Five samples were collected from different sampling points. Samples were homogenized, then mixed and an average sample of 1000 ml was studied. From this average sample there were prepared three solutions of different concentrations: 1% (v/v), 3 % (v/v) and 6 % (v/oJ diluted with waste water coming from the waste water treatment plant, whose composition meet the regulation for a wastewater treatment plant. On these samples it has been studied the decreasing of contaminants concentration after the treatment with chemical flocculants: FeSO4 3%, Ca(OH)2 20% and IP114 polyelectrolyte 0.2 ppm. After physical and chemical treatment the highest concentration sample was analyzed, focusing on contaminants biodegradability in contact with biological sludge by biological treatment using a pilot plant.


Gheorghe C.G.,SN Pasteur SA Filipesti subsidiary | Pantea O.,Petroleum Gas University of Ploiesti | Matei V.,Petroleum Gas University of Ploiesti | Bombos D.,Petroleum Gas University of Ploiesti | Borcea N.-F.,Petroleum Gas University of Ploiesti
Revista de Chimie | Year: 2011

In this experiment we tested separately the evolution of turbidity for two pure bacterial suspensions, Bacillus subtilis ATCC 2589 and Pseudomonas aeruginosa ATCC 15442, in a concentration of 1 Mc Fariand. In comparison with the evolution of pure suspensions we have analyzed the evolution of suspension turbidities in contact with diesel fuel in different concentrations: 100μL Diesel, 200μL Diesel and 300μL Diesel. The samples were incubated at 30-37 degrees Celsius for 72 h; after 6 h, 24, 48 and 72 h thermostating time, bacterial suspension was photo metered at a wave with 530 nm length for measuring the suspension turbidity. In order to determine the viability of the microorganisms tested in the presence of the analyzed contaminators were made counts of bacterial colonies grown on nutrient agar culture medium (CFU/ml).


Gheorghe C.G.,SN Pasteur SA Filipesti subsidiary | Pantea O.,Petroleum Gas University of Ploiesti | Matei V.,Petroleum Gas University of Ploiesti | Bombos D.,Petroleum Gas University of Ploiesti | Borcea A.-F.,Petroleum Gas University of Ploiesti
Revista de Chimie | Year: 2011

A series of microbiological tests have been made in order to test the toxicity degree of cationic detergents on some bacterial strains which are present in the biocoenosis in a water treatment plant. In this study we tried the selection of the best of the fungal and bacterial strains tested, which have the ability to resist this kind of contamination and can help the biological sludge in a water treatment plant through the stabilization of biocenosis against the attack of the contaminators. Tests were made on three cationic detergents: chloride alkyl (C12-C16), dimethyl-benzyl ammonium chloride and ammonium didecildimetil, in the presence of the following bacterial and fungal strains: Micrococcus luteus ATCC 9341, Pseudomonas aeruginosa ATCC15442, Bacilus subtilis NCTC 6633, Aspergillus niger ATCC 16404 and Candida albicans ATCC 10231. Bacterial suspension which had to be tested, was added to a sample of solutions of detergents with different concentrations, and then the initial concentrations and the concentrations during the contact with the contaminators were analyzed.


Gheorghe C.G.,SN Pasteur SA Filipesti Subsidiary | Pantea O.,Petroleum Gas University of Ploiesti | Matei V.,Petroleum Gas University of Ploiesti | Bombos D.,Petroleum Gas University of Ploiesti | Borcea A.-F.,Petroleum Gas University of Ploiesti
Revista de Chimie | Year: 2011

In the study was followed the evolution of microorganisms population in biological sludge on a contaminated water with contaminators with known concentrations, maintaining its life at optimum climate. Tests were conducted to Find answers on the ability of microorganisms to biodegrade different contaminators and to Find different microbial strains able to degrade certain contaminators. The identification of specific strains for each contaminator separately help to restore biocenosis from a wastewater treatment plant that could be affected by pollution incidents and can help the effective biodegradation of wastewater. This experiment studies biodegradation of contaminators in presence of wastewater coming from the water treatment plant using biological sludge adapted to this kind of contaminators evolution of such systems to eliminate pollutants in two comparative treatments: the treatment with biological sludge and the second system by adding activated charcoal into the biological sludge.

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