Verandani S.,Smt Chm College |
Vardhan D.,Ramnarain Ruia College
Research Journal of Chemistry and Environment | Year: 2012
Water samples were collected from 25 sampling sites during the pre-monsoon season in Ulhasnagar city, Thane district in Mumbai. Different physicochemical parameters were measured and the samples were also analyzed for dissolved metal concentrations. The surface water physicochemical parameters included pH, temperature, turbidity, hardness, total alkalinity, dissolved oxygen (DO), total dissolved solids (TDS), electrical conductance (EC) and turbidity while the dissolved metals were Cd, Cu, Pb and Cr. The ranges for the physicochemical parameters were 28-32 degrees C for temperature, 6.8-8.5 for pH, 0.4-12.8 mg/l for DO, 0-100 NTU for turbidity, 22-580 ppm (as CaCO3) for hardness, 44-61 ppm. for total alkalinity, 250-520 mg/l for TDS and 554-1040 mhos/cm while the dissolved metals (mg/l) were 0.001-0.008 for Cd, 0.002-0.012 for Cu, 0.04-1.30 for Pb and 0.000-0.15 for Cr. Based on multivariate analysis and correlation studies, lead was identified as major dissolved metal contaminant in most of the analyzed samples. Microbial analysis was also carried out by testing the samples for E. coli and thermo tolerant bacteria and ranges for these were from 2-18 ppm for E. Coli and 2-19 ppm for the thermo tolerant bacteria.
Waghela B.N.,Indian Institute of Advanced Research |
Sharma A.,Indian Institute of Advanced Research |
Dhumale S.,Indian Institute of Advanced Research |
Pandey S.M.,Smt Chm College |
Pathak C.,Indian Institute of Advanced Research
PLoS ONE | Year: 2015
Curcumin, an ingredient of turmeric, exhibits a variety of biological activities such as anti-inflammatory, anti-atherosclerotic, anti-proliferative, anti-oxidant, anti-cancer and anti-metastatic. It is a highly pleiotropic molecule that inhibits cell proliferation and induces apoptosis in cancer cells. Despite its imperative biological activities, chemical instability, photo-instability and poor bioavailability limits its utilization as an effective therapeutic agent. Therefore, enhancing the bioavailability of curcumin may improve its therapeutic index for clinical setting. In the present study, we have conjugated curcumin with a biodegradable polymer Poly (D, L-lactic-co-glycolic acid) and evaluated its apoptotic potential in human colon carcinoma cells (HCT 116). The results show that curcumin-PLGA conjugate efficiently inhibits cell proliferation and cell survival in human colon carcinoma cells as compared to native curcumin. Additionally, curcumin conjugated with PLGA shows improved cellular uptake and exhibits controlled release at physiological pH as compared to native curcumin. The curcumin-PLGA conjugate efficiently activates the cascade of caspases and promotes intrinsic apoptotic signaling. Thus, the results suggest that conjugation potentiates the sustainability, anti-proliferative and apoptotic activity of curcumin. This approach could be a promising strategy to improve the therapeutic index of cancer therapy. © 2015 Waghela et al.
Bajpai S.K.,P.A. College |
Das P.,Smt Chm College |
Sharma L.,P.A. College
Journal of Macromolecular Science, Part A: Pure and Applied Chemistry | Year: 2013
In this work, cotton cellulose/polyacrylic (CC/PAAc) composite fibers are produced by soaking plain cotton cellulose (CC) fibers in a reaction mixture containing monomer acrylic acid, crosslinker N,N-methylene-bis acrylamide (MB) and initiator potassium persulphate (KPS), followed by microwave induced in-situ formation of polyacrylic acid within cotton fibers. The fibers are characterized by TGA, FTIR and SEM analysis. The mechanical strength of CC/PAAc fibers was comparable to that of CC fibers, indicating marginal loss in mechanical strength. The antibacterial drug Gentamicin Sulphate (GS) was added to the reaction mixture before soaking the CC fibers in the reaction solution. This yielded GS loaded CC/PAAc fibers. The release of drug GS was investigated as a function of the amount of crosslinker in the soaking solution. It was found that the quantity of crosslinker MB in the soaking solution influenced the release mechanism. Fiber samples with relatively lower and higher amount of crosslinker followed Second order model and the Higuchi diffusion model, respectively. The CC fibers showed lower release as compared to the CC/PAAc fibers. Finally, the biocidal action of fibers was tested using a zone of inhibition method using E. Coli as model bacteria. The fibers demonstrated biocidal action by exhibiting zones of inhibition of diameter 1.3 and 2.2cm, respectively for fibers prepared by soaking in the reaction solutions containing 0.2 and 0.4 percent (w/v) drug. Finally, the killing kinetics was investigated to show time dependent bacterial killings for the fiber samples GSLCC/PAAc (0.2) and GSLCC/PAAc (1.2). © 2013 Copyright Taylor and Francis Group, LLC.
Bajpai S.K.,Robertson College, Jabalpur |
Das P.,Smt Chm College
Journal of Applied Polymer Science | Year: 2011
This study describes preparation of poly (acrylic acid)-grafted cotton fibers and release of antibiotic drug gentamicin sulfate from them under physiological conditions. Poly(acrylic acid) has been grafted onto cellulose backbone of cotton fibers via Ce(IV)-initiated polymerization in aqueous medium. The conditions obtained for optimum grafting were as follows: initiation time 30 min; initiation temperature 37°C; monomer concentration 27.8 mM; grafting temperature 30°C; nitric acid (catalyst) concentration 0.1M. The grafted fibers were characterized by FTIR, TGA, and SEM analysis. The antibiotic drug gentamicin sulfate (GS) was loaded into the grafted fibers by equilibration method and release was studied under physiological conditions. The kinetic release data was interpreted by first-order kinetic model. Finally, drug-loaded fibers showed fair antibacterial action against Escherichia coli. © 2011 Wiley Periodicals, Inc.
Bajpai S.K.,P.A. College |
Das P.,Smt Chm College |
Soni B.,Polymer Research Laboratory
Journal of Industrial Textiles | Year: 2016
In this work, poly(acrylic acid) has been grafted onto cotton fibers through the free radical initiated polymerization and the resulting fibers have been characterized by Fourier transform infrared, thermogravimetric analysis, and scanning electron microscopy analysis. The grafted fibers have been loaded with copper nanoparticles using in situ approach. The Transmission Electron Microscopy (TEM) analysis of Cu nanoparticles revealed that almost 45% of the particles had a diameter range of 60–80 nm. The copper nanoparticles loaded fibers show slow release of Cu(II) ions, extended over a period of around 50 h. The release of Cu(II) ions followed a second-order kinetic model successfully. The fibers also exhibited an excellent antibacterial action against model bacteria Escherichia coli as tested by zone of inhibition method. © 2014, The Author(s) 2014.