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Saint Petersburg, Russia

Gololobov V.G.,Sm Kirov Military Medical Academy
Genes and Cells | Year: 2014

The goal of the study was to analyse the features of tissue reactions during reparation of the bone wound following gunshot long bone fracture in human. Analysis of biopsy specimens, i.e. bone splinters, edges of bones fragments and surrounding them tissues taken from 9 male patients undergoing surgical treatment due to gunshot fracture of long bones has been carried out. Histological study using light and electron microscopy has been performed on the 1st, 2d, 3d, 4th, 5th, 14th, 23d and 34th days following the injury. It has been shown that newly developed blood vessels are capable for transportation of osteogenic cambial cells into the zone of the fracture thus optimizing osteogenesis. Regenerative endossal osteogenesis found out in this study proved to promote reconstructed osteons formation missing a stage of reticulofibrous bone tissue. It has been shown also that cells carrying osteogenic properties introduced into retained splinters during re-vascularization are responsible for the bone tissue construction. Such fragments served as additional and considerable source of bone regenerate formation. They are considered to be so-called «post-injury organ culture» in vivo. Difference between development of regeneration zone from distal and proximal edges of fractured bones was documented. Ultrastructural changes within osteoblasts, osteocytes and extracellular matrix reflected the intensity of bone tissue formation. Our results have been taken into consideration in routine clinical management and methods of treatment among such patients have been proposed. Source

Shestakova M.V.,Moscow State University | Khalimov Y.S.,Sm Kirov Military Medical Academy
Terapevticheskii Arkhiv | Year: 2013

The review gives detailed information on the state-of-the-art of hypoglycemia (HG) and the results of its Russian and international trials. It is noted that HG should be now regarded as an extremely undesirable glucose-lowering therapy complication that is closely related to the risk of cardiovascular events and higher mortality rates, worse glycemic control, and poorer quality of life in patients with type 2 diabetes mellitus (T2DM). The priority effective therapy tactics for T2DM is to manage the latter without any risk of HG, which can be implemented by the wide clinical application of incretins, dipeptidyl peptidase-4 inhibitors in particular, which have the glucose-lowering activity comparable with that shown by metformin and sulfonylurea drugs, are practically safe when used as monotherapy and significantly enhance the efficiency of therapy without substantially increasing the risk of severe HG when co-administered with other medications. Source

Gololobov V.G.,Sm Kirov Military Medical Academy
Genes and Cells | Year: 2015

The 130th anniversary of academician A.A. Zavarzin, the founder of evolutional histology and the author of fundamental theory of parallel series of tissue development will held in 2016. Being an outstanding scientist and organizer, he managed to create a powerful school of histologists-evolutionists in the Soviet Union. The paper contains short variant of history foundation and analysis of the most outstanding achievements of his school, which are contributing towards perspective development of histology. Source

Sivolodskii E.P.,Sm Kirov Military Medical Academy
Klinichescheskaya Laboratornaya Diagnostika | Year: 2015

The chromogenic synthetic medium «Klebsiella 5-ASK CHROM-C» was developed for isolation and identification of klebsiellae of species of K.pneumoniae subsp. pneumoniae, K.oxytoca, K.mobilis according chromogenic reaction to enzyme 5-aminosalycilate decarboxylase as a unique marker of genus Klebsiella. The L-proline and L-calcium glutamate are used as a source of nitrogen and carbon in medium. The consistency of composition of growth medium that ensure its regularity. The diagnostic sensitivity of chromogenic medium is 95.3±1. 7%; diagnostic specificity is 100%; analytical sensitivity is 1-2 colony-forming units per ml-1. The identification of Klebsiella is achieved simultaneously with their isolation during 24=48 hours. The test of 5-ASK decarboxylase using two chromogenic mediums ((Klebsiella 5-ASK CHROM-C» permits identifying additionally K.pneumoniae subsp, ozaenae, K.pneumoniae subsp. Rhinoscleromatis. Source

Segizbaeva M.O.,RAS Pavlov Institute of Physiology | Donina Z.A.,RAS Pavlov Institute of Physiology | Timofeev N.N.,Military Sport Institute 63 | Korolyov Y.N.,Sm Kirov Military Medical Academy | And 2 more authors.
Advances in Experimental Medicine and Biology | Year: 2013

The aim of this study was to characterize the pattern of inspiratory muscle fatigue and to assess the resistance to fatigue of the diaphragm (D), parasternal (PS), sternocleidomastoid (SCM), and scalene (SC) muscles. Nine healthy, untrained male subjects participated in this study. Electromyographic activity (EMG) of D, PS, SCM, and SC was recorded during an incremental cycling test to exhaustion (workload of 1.0 W/kg with 0.5 W/kg increments every 5 min). The before-to-after exercise measurements of maximal inspiratory pressure (MIP) and EMG power spectrum changes were performed. The maximal inspiratory pressure declined about 8.1 % after exercise compared with that in the control condition (124.3 ± 8.5 vs. 114.2 ± 8.9 cmH2O) (P > 0.05), whereas the peak magnitude of integrated electrical activity of D, PS, SCM, and SC during the post-exercise Müller maneuver was significantly greater in all subjects than that pre-exercise. The extent of inspiratory muscles fatigue was evaluated by analysis of a shift in centroid frequency (fc) of EMG power spectrum. Exercise-induced D fatigue was present in three subjects and PS fatigue was another in two; whereas both D and PC fatigue were observed in four subjects. All subjects demonstrated a significant reduction in f c of SCM and SC. Results indicate that early signs of the fatiguing process might be detected in the D, PS, SCM, and SC muscles during exercise to exhaustion. Fatigue of either D or PS muscles develops selectively or together during exhaustive exercise, depending on the recruitment pattern of respiratory muscles. Accessory inspiratory muscles of the neck are less resistant to fatigue compared with the D and PS muscles. © 2013 Springer Science+Business Media Dordrecht. Source

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