SLT Institute of Pharmaceutical science

Bilāspur, India

SLT Institute of Pharmaceutical science

Bilāspur, India
SEARCH FILTERS
Time filter
Source Type

Omray L.K.,Sagar Institute of Pharmaceutical science | Jain S.K.,SLT Institute of Pharmaceutical science
Indian Journal of Pharmaceutical Sciences | Year: 2010

The purpose of the present work was to design and optimize floating drug delivery systems of acyclovir using psyllium husk and hydroxypropylmethylcellulose K4M as the polymers and sodium bicarbonate as a gas generating agent. The tablets were prepared by wet granulation method. A 3 2 full factorial design was used for optimization of drug release profile. The amount of psyllium husk (X1) and hydroxypropylmethylcellulose K4M (X2) were selected as independent variables. The times required for 50% (t 50%) and 70% (t70%) drug dissolution were selected as dependent variables. All the designed nine batches of formulations were evaluated for hardness, friability, weight variation, drug content uniformity, swelling index, in vitro buoyancy, and in vitro drug release profile. All formulations had floating lag time below 3 min and constantly floated on dissolution medium for more than 24 h. Validity of the developed polynomial equation was verified by designing two check point formulations (C1 and C2). The closeness of predicted and observed values for t50% and t 70% indicates validity of derived equations for the dependent variables. These studies indicated that the proper balance between psyllium husk and hydroxypropylmethylcellulose K4M can produce a drug dissolution profile similar to the predicted dissolution profile. The optimized formulations followed Higuchi's kinetics while the drug release mechanism was found to be anomalous type, controlled by diffusion through the swollen matrix.


Tewatia N.,Bundelkhand University | Abida,Advanced Institute of Pharmacy | Namdeo K.P.,SLT Institute of Pharmaceutical science
Journal of Chemical and Pharmaceutical Research | Year: 2012

A series of pyrazine carboxamide derivatives was synthesized by the condensation of pyrazine-2-carboxylic acid chloride with various substituted amino pyridines. The structures of these derivatives were elucidated on the basis of IR, 1H-NMR and mass spectral data. These compounds were further evaluated for their antimycobacterial activity and antifungal activity.


Pandey R.S.,SLT Institute of Pharmaceutical science | Jain V.,Famycare Ltd | Katare O.P.,Panjab University | Chandra R.,University of Delhi | Katyal A.,University of Delhi
Nanomedicine: Nanotechnology, Biology, and Medicine | Year: 2013

Noscapine crosses blood-brain-barrier and inhibits proliferation of glioblastoma cells. However, short plasma half-life and rapid elimination necessitate the administration of multiple injections for successive chemotherapy. Noscapine bearing solid lipid nanoparticles, Nos-SLN and poly (ethylene)-glycol conjugated solid lipid nanoparticles of noscapine, Nos-PEG-SLN of 61.3±9.3-nm and 80.5±8.9-nm containing 80.4±3.2% and 83.6±1.2% of Nos, were constructed. First order kinetic and Higuchi equation were followed to release the Nos at intracellular pH~4.5. Further, a decrease in IC50 (Nos; 40.5μM>Nos-SLN; 27.2μM>20.8μM) and enhanced subG1 population were observed in U87cells. Plasma half-life was enhanced up to ~11-fold and ~5-fold by Nos-PEG-SLN and Nos-SLN which significantly (P<0.05) deposits 400.7μg/g and 313.1μg/g of Nos in comparison to 233.2μg/g by drug solution. This is first report demonstrating a workable approach to regulate the administration of multiple injections of Nos, warranting further in vivo tumor regression study for superior management of brain cancer. From the Clinical Editor: This report describes a possible approach to regulate the administration of multiple injections of Noscapine using solid lipid nanoparticles. The data warrant further in vivo tumor regression studies for optimal management of glioblastoma, a generally very poorly treatable brain cancer. © 2013 Elsevier Inc.


Madan J.,Georgia State University | Pandey R.S.,SLT Institute of Pharmaceutical science | Katare O.P.,Panjab University | Aneja R.,Georgia State University | Katyal A.,University of Delhi
Colloids and Surfaces B: Biointerfaces | Year: 2013

Noscapine, recently identified as anticancer due to its microtubule-modulating properties. It is presently in Phase I/II clinical trials. The therapeutic efficacy of noscapine has been established in several xenograft models. Its pharmacokinetic limitations such as low bioavailability and high ED50 impede development of clinically relevant treatment regimens. Here we present design, synthesis, in vitro and in vivo characterization of sterically stabilized gelatin microassemblies of noscapine (SSGMS) for targeting human non-small cell lung cancer A549 cells. The average size of the sterically stabilized gelatin microassemblies of noscapine, SSGMS was 10.0±5.1μm in comparison to noscapine-loaded gelatin microassemblies, GMS that was 8.3±5.5μm. The noscapine entrapment efficiency of SSGMS and GMS was 23.99±4.5% and 24.23±2.6%, respectively. Prepared microassemblies were spherical in shape and did not show any drug and polymer interaction as examined by FTIR, DSC and PXRD. In vitro release data indicated that SSGMS and GMS follow first-order release kinetics and exhibited an initial burst followed by slow release of the drug. In vitro cytotoxicity evaluated using A549 cells showed a low IC50 value of SSGMS (15.5μM) compared to GMS (30.1μM) and free noscapine (47.2μM). The SSGMS can facilitate a sustained therapeutic effect in terms of prolonged release of noscapine as evident by caspase-3 activity in A549 cells. Concomitantly, pharmacokinetic and biodistribution analysis showed that SSGMS increased the plasma half-life of noscapine by ∼9.57-fold with an accumulation of ∼48% drug in the lungs. Our data provides evidence for the potential usefulness of SSGMS for noscapine delivery in lung cancer. © 2013 Elsevier B.V.


Soni M.L.,SLT Institute of Pharmaceutical science | Soni M.L.,Gyani Inder Singh Institute of Professional Studies | Gupta M.,University of Selangor | Namdeo K.P.,SLT Institute of Pharmaceutical science
Chemical Papers | Year: 2016

The study sought to isolate asporopollenin-like biopolymer from Aspergillus Niger (A. Niger) spore. The spore exine (Sp-exine) from A. Niger was isolated using four different methods. The highest isolation efficiency (9.32 %) was found for the method based on H2SO4 treatment. Optical microscopy, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images showed that the isolated Sp-exine had a spherical shape with a spun morphology and was highly uniform in size. The elemental compositions of the bodies of the Sp-exine materials, as well as their surfaces, were observed by means of CHN combustion analysis and SEM with energy-dispersive X-ray spectroscopy (SEM-EDX), respectively. The result demonstrates C, H and O to be the main structural elements in all the Sp-exine samples. Fourier-transform infrared (FTIR) spectroscopy coupled with solid state 13C nuclear magnetic resonance (solid state 13C NMR) spectroscopy and UV-Vis spectroscopy were used to compare the changes in the functional groups of the Sp-exine samples isolated using the different methods. A thermogravimetric analysis (TGA) study demonstrated all the Sp-exine samples showed high thermal stability. Among all tested methods, the treatment with H2SO4 and alcoholic potassium hydroxide exhibited the best results in removing cellular and other contents with minimal drastic effect on the structure and morphology and is proposed as the best method for isolating Sp-exine since it required minimal isolation time and showed good isolation efficiency. © 2016 Institute of Chemistry, Slovak Academy of Sciences.


Karan S.K.,Seemanta Institute of Pharmaceutical science | Mondal A.,Indian Institute of Chemical Technology | Mishra S.K.,Utkal University | Pal D.,SLT Institute of Pharmaceutical science | Rout K.K.,Indian Institute of Chemical Technology
Pharmaceutical Biology | Year: 2013

Context: In the Indian traditional system of medicine, Streblus asper Lour (Moraceae) is prescribed for the treatment of diabetes mellitus. Objective: In the present study, α-amyrin acetate isolated from S. asper, and the petroleum ether extract of S. asper stem bark (PESA) was screened for their antidiabetic properties in streptozotocin (STZ)-induced diabetic rats. Materials and methods: Successive Soxhlet extraction of the dried stem bark with petroleum ether and then with ethanol (95%) yielded petroleum ether and ethanol extracts, respectively, which were concentrated under reduced pressure. Hyperglycemia was induced in rats by STZ (50mg/kg, b.w.). Twenty-four hours after STZ induction, respective groups of diabetic rats received PESA (100, 250 and 500mg/kg, b.w.) and α-amyrin acetate (25, 50 and 75mg/kg, b.w.) respectively, orally daily for 15 days. Glibenclamide (0.5mg/kg, orally) served as a reference. Blood glucose levels were measured on every 5th day during the 15 days of treatment. The serum lipid profiles and biochemical parameters, viz., serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP), insulin and glycosylated hemoglobin level, were measured. Results: PESA significantly (p < 0.01) normalized blood-glucose levels and serum biochemical parameters as compared with those of STZ controls. α-Amyrin acetate (75mg/kg, b.w.) exhibited maximum glucose lowering effect (71.10%) in diabetic rats compared to the other dose (25, 50mg/kg) at the end of the study. The protective effect was further confirmed by histopathological examination of the liver. Conclusion: PESA and α-amyrin acetate demonstrated remarkable antidiabetic activity in STZ-induced diabetic rats. © 2013 Informa Healthcare USA, Inc.


Jain R.,SLT institute of Pharmaceutical science | Jain S.K.,SLT institute of Pharmaceutical science
International Journal of Phytomedicine | Year: 2010

An attempt has been made to review some medicinal plants used for the prevention and treatment of cancer in Chhattisgarh. Information on the name of plants, family, parts used and method of preparation has been collected from Ethanobotanical literatures. Information collected has revealed 53 plants species that are used for treatment of cancer in Chhattisgarh. All these plants were further reviewed for scientific evidence, 33 plants out of 53 plants were found for possess anticancer, cytotoxic or antioxidant activity in various preclinical or clinical studies. ©arjournals.org, All rights reserved.


Verma S.,Slt Institute Of Pharmaceutical Science | Dangi J.S.,Slt Institute Of Pharmaceutical Science
Research Journal of Pharmacy and Technology | Year: 2011

The delivery of poorly water soluble drugs has been the subject of much research, as approximately 40% of new chemical entities are hydrophobic in nature. In the present study, an attempt has been made to develop non aqueous microemulsion of the type oil-in polyethylene glycol, stabilized by surfactant with suitable drug loading. Non aqueous microemulsion have attracted a great deal of attention not only because of there importance in industrial application but also their intrinsic interest. They optimize the performance of a wide spectrum of products and processes. non aqueous microemulsion are suitable for poorly aqueous soluble drugs and thermodynamically stable multicomponent fluids composed of polar solvent, oil and mixture of a liquid. Administration of drugs is one of the convenient and often advantageous delivery, especially when dealing with children or the elderly for whom pill swallowing can be difficult or even hazardous. Unfortunately many drugs are not soluble in water, while water solution of it may have an unpleasant taste. Some drugs are either unstable in the presence of water or are insoluble in water and therefore cannot be incorporated into aqueous formulations. To overcome these various problems a water free liquid preparation of a number of drugs would be desirable. Two basics strategies could be considered when searching for stable non-aqueous emulsions. One is to design surfactants having two incompatible blocks, each of which is selectively soluble in either of the immiscible liquids. The other approach is to search for a suitable oil immiscible polar liquid that can substantially replace water using emulsifiers. Characterization of non aqueous or anhydrous or oil- in- oil microemulsions shows uniform size distribution of vesicles in micron range with avoidance of phase separation. Release profile shows sustained action for extend period of time. © RJPT All right reserved.


Raj N.,SLT Institute of Pharmaceutical science | Jain S.K.,SLT Institute of Pharmaceutical science
International Journal of ChemTech Research | Year: 2012

A set of forty one substituted-2-phenyl-benzimidazole derivatives with anti-allergic activity was subjected to the two dimensional quantitative structure activity relationships studies using V life molecular design suite 3.5. Drug Designing module contain various combinations of thermodynamic, electronic, topological and spatial descriptors.Substituted-2-phenyl-benzimidazole was taken as the lead molecule and QSAR model developed using partial least square regression approach. For each set of descriptors, the best multilinear.QSAR equations were obtained by the stepwise forward backward variable selection method. Logarithmic inverse value of IC50 was taken as dependent variable and various physico-chemical descriptors were taken as independent variable. The best QSAR model i.e. model-2(r 2 = 0.7107, Fischer's test value F=17.8671, r 2 se = 0.4900) has acceptable statistical quality and predictive potential as indicated by the value of cross validated squared correlation coefficient (q 2=0.6230) and r 2 for external test set (pred_r 2 = 0.7256). From the build model it seems to be clear that SssNHE- index, IPC Average and SaasN(Noxide)E-index contributes negatively and SssCH 2E-index,slogp and chi6chain contributes positively. Thus this validated model brings important structural insight to aid the design of more potent anti-allergic agents.


Raj N.,SLT Institute of Pharmaceutical science | Jain S.K.,SLT Institute of Pharmaceutical science
Digest Journal of Nanomaterials and Biostructures | Year: 2011

A set of forty one substituted 2-phenyl-benzimidazole with anti allergic activity against IgE was subjected to three dimensional quantitative structure activity relationship studies through recently introduced k- nearest neighbor molecular field analysis with step wise forward-backward as variable selection method to study the correlation between the molecular properties and the In-vitro IgE activities. In the present study k-NN-MFA calculations for both electrostatic and steric field were carried out. The master grid maps derived from the best model has been used to display the contribution of electrostatic potential and steric field. The k-NN-MFA models obtained by using 90% of training set selection showed that electrostatic and steric interactions play major role in determining biological activity. The statistical results showed significant correlation coefficient r 2 (q 2) of 0.5757, r 2 for external test set (pred_r 2) 0.7238, coefficient of correlation of predicted data set (pred_r 2se) of 0.5799, degree of freedom 33 and k nearest neighbor of 2.The k-NN MFA contour plots provided further understanding of the relationship between the structural features of substituted-2-phenyl-benzimidazole derivatives and their activities, which should be applicable to design new, potential anti allergic agents.

Loading SLT Institute of Pharmaceutical science collaborators
Loading SLT Institute of Pharmaceutical science collaborators