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Bratislava, Slovakia

Babalova M.,Slovak University of Medicine | Blahova J.,Slovak University of Medicine | Kralikova K.,Slovak University of Medicine | Krcmery V.,Slovak University of Medicine | Jezek P.,Regional Hospital Pribram
Lekarsky Obzor | Year: 2011

Background: Transfer of resistance to imipenem and/or to fluoroquinolones from resistant bacterial strains to susceptible bacteria can decrease their value as effective reserve antibiotics. Therefore, it is important to monitor the incidence of transferable resistance to these drugs in nosocomial isolates of severely ill patients hospitalized in clinical settings. Aim of study: To demonstrate the transferability of multi-resistant bacteria to cephalosporin antibiotics (in strains producing ESBL) and resistant to imipenem and/or fluoroquinolones. To study also the process of mobilization for transfer of these antibacterials by other transferable genetic determinants, e. g. by genes for ESBL. Methods: We used mixed cultures of multi-resistant wild-type donor strains with rifampicin-resistant but otherwise susceptible recipient strain of E. coli K-12. Transconjugant clones were selected on bi-antibiotic media (rifampicin plus an antibiotic in the spectrum of resistance of the donor strain) and were tested for the presence of all other resistance determinant present in the spectrum of the donor strain. Results: In one strain of Enterobacter the resistance to imipenem and ofloxacin was mobilized by the transfer of genes coding for ESBL. A strain of Providencia was mobilized for transfer of resistance to fluoroquinolones by use of two donor strains. Conclusion: Our previous observation of mobilization of transfers to fluoroquinolones by genes coding for the production of ESBL was confirmed in strains from patients of another large Regional Hospital and was extended by the demonstration of mobilization also of resistance to imipenem.

Serbin R.,Slovak University of Medicine | Uhnakova I.,Slovak University of Medicine | Husekova Z.,Slovak University of Medicine | Ursinyova M.,Slovak University of Medicine
Chemicke Listy | Year: 2011

A simple method for methylmercury (MeHg+) determination in human venous blood is described using GCICP- MS. The blood sample preparation consists in the extraction with a mixture of 6M HCl and NaCl, pH adjustment, derivatization of mercury species using NaBPh4 with simultaneous extraction of products into hexane. The detection limits for MeHg+ were 86 ppt (as Hg) in the optimized method. The sample volume for repeated measurements was 150 μl. The total Hg level in blood was determined by AAS using the amalgamation technique.

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