Slovak University of Agriculutre in Nitra

Nitra, Slovakia

Slovak University of Agriculutre in Nitra

Nitra, Slovakia
SEARCH FILTERS
Time filter
Source Type

Hudz N.,Lviv National Medical University | Ivanova R.,Moldova Academy of Sciences | Brindza J.,Slovak University of Agriculutre in Nitra | Grygorieva O.,Ukrainian Academy of Sciences | And 2 more authors.
Potravinarstvo Slovak Journal of Food Sciences | Year: 2017

The main objective of this study was to develop approaches for the determination of total antioxidant activity of natural products (bee bread and safflower extracts) using DPPH radical scavenging assay. Considering that analytical procedures and results related to this assay and reported by many authors are significally differed between each other and depend on many factors (the nature of tested extracts, the nature of solvents for extraction, a reaction time of DPPH with a sample, DPPH solvents and concentration, ratio between DPPH and an extract, etc.), the methodology of the evaluation of antioxidant capacity of different origin extracts by DPPH radical scavenging assay was developed. Ascorbic acid (AA) was used as standard antioxidant and the correlation between the percentage of DPPH scavenging and AA concentration was determined at two different initial absorbances of DPPH solution. Average concentration of AA which inhibited 50% of DPPH radicals (IC50) was equal to 156.0 - 171.26 μg.mL-1. The reaction kinetics of DPPH inhibition by bee bread and safflower extracts was described by the curves of the dependence of the total antioxidant activity on time with squared correlation coefficients (R2) in the range of 0.89 - 0.98. The reaction times for these extracts were from 40 to 70 min at the correct ratio of volumes between the tested extracts and a DPPH solution. These studies demonstrated that the extracts obtained from bee bread of 2016 year of pollen collection had significantly higher the total antioxidant activity compared with the extracts of bee bread of pollen collection of 2015 considering the ratio of bee bread and the solvent in the extracts and volume of the extract for the procedure. This fact is explained not only botanical origin bun also the time of the storage of bee bread before the preparation of extracts. There was not found significant differences in the total antioxidant activity of extracts from flowers of safflower sown in fall and in spring. Antioxidant activity of the extracts from leaves of spring sown safflower was higher compared with the total antioxidant activity of the extracts from fall sown plants. Ascorbic acid equivalents of the tested extracts could be ranged as follows: bee bread of 2016 pollen collection > bee bread of 2015 pollen collection > leaves of safflower spring sown > flowers of safflower spring sown > flowers of safflower fall sown > leaves of safflower fall sown. © 2017 Potravinarstvo Slovak Journal of Food Sciences.


Monka A.,Human Resources University | Grygorieva O.,Ukrainian Academy of Sciences | Chlebo P.,Human Resources University | Brindza J.,Slovak University of Agriculutre in Nitra
Potravinarstvo | Year: 2014

Quince (Cydonia oblonga Mill.) is a fruit species, whose fruits have a high therapeutic value and therefore are used in many countries in traditional medicine. Chinese quince (Pseudocydonia sinensis Schneid.) is a lesser used species, although it is a relative of quince. The aim of the study was to evaluate some morphological characters of both kinds of fruit and antioxidant activity of morphological parts of the fruit. For these experiments, two genotypes were used from each species growing in the Arboretum Mlynany (Slovakia). We determined the antioxidant activity of different parts by the DPPH method. In the genotypes from the evaluated species C. oblonga/P. sinensis we determined the average weight of the fruit in the fresh condition to be in the range 147.61-253.27 g/197.85-466.38 g, the exocarp weight 28.50-43.89 g/24.85-45.10 g, the mesocarp weight 116.36-204.99 g/160.30-389.80 g, the seed weight 1.05-1.54 g/9.22-17.42 g, the height of fruit 74.09-80.88 mm/98.06-124.48 mm, average of fruit 60.11-81.51 mm/62.33-88.64 mm. In aqueous extracts we determined antioxidant activity of the species C. oblonga/P. sinensis in dry exocarp in the range 43.52-67.73%/52.76-82.20%, in fresh mesocarp 7.36-14.78%/15.30-23.50%, in dry mesocarp 30.92-41.30%/41.68-50.15% and dry endocarp 55.19-76.44%/91.20-92.72%. We determined antioxidant activity in methanolic extracts of the species C. oblonga/P. sinensis in dry exocarp in the range 93.29-93.32%/91.87-93.25%, in fresh mesocarp 10.29-36.0%/17.10-17.11%, in dry mesocarp 54.55-74.11%/80.39-84.11% and in dry endocarp 95.14-95.39%/94.97-95.62%. Results document that the fruits of both species can be practically used in the preparation of many dishes, while they can be used as raw material for pharmaceutical and cosmetic use. © 2014 Potravinarstvo. All rights reserved.


Grygorieva O.,Ukrainian Academy of Sciences | Abrahamova V.,Slovak University of Agriculutre in Nitra | Karnatovska M.,Nikita Botanical Garden Research Center | Bleha R.,Institute of Chemical Technology Prague | Brindza J.,Slovak University of Agriculutre in Nitra
Potravinarstvo | Year: 2014

The aim of this work is to discriminate 28 Ziziphus jujuba Mill. genotypes (ZJ-01-ZJ-28) based on weight and morphological characteristics of fruit, stones and seeds. These genotypes maintained in a clone repository (Nova Kachovka, Ukraine) were chosen for investigation as potential species for cultivation and use in Slovakia. The fruits were collected at the period of their full maturity (October); their morphometric parameters were following: weight from 2.90 g (ZJ-13) to 28.99 g (ZJ-22), length from 20.73 mm (ZJ-08) to 44.84 mm (ZJ-04), and width from 16.64 mm (ZJ-13) to 38.87 mm (ZJ-22). Isolated stones were also analysed, and their weight was from 0.173 g (ZJ-07) to 0.731 g (ZJ-20), length from 12.84 mm (ZJ-08) to 28.67 mm (ZJ-04), and width from 5.06 mm (ZJ-07) to 9.74 mm (ZJ-01). The weight of the seeds was ranging from 20.00 mg (ZJ-25) to 69.11 mg (ZJ-12), length from 7.24 mm (ZJ-15) to 11.85 mm (ZJ-04), and width was from 3.86 mm (ZJ-05) to 6.84 mm (ZJ-12). Significant differences in the size, shape and color of fruits, stones and seeds were also identified. The pulp, seedless stones and seeds constitute 92.81% (ZJ-26) - 98.32% (ZJ-04), 1.36% (ZJ-04) - 6.27% (ZJ-20), and 0.25% (QA-24, QA-25) - 1.21% (ZJ-16) of the total fruits weight, respectively The shape index of the fruits was found in the range of 1.04 (ZJ-08) to 2.00 (ZJ-12 and ZJ-25). The shape indexes of stones and seeds were found ranging from 1.74 (ZJ-08) to 4.53 (ZJ-05) and from 1.32 (ZJ-23) to 2.74 (ZJ-04), respectively. The relative weight of pulp was determined in the range from 92.76% (ZJ-13) to 98.32% (ZJ-04) and the relative weight of seed was determined in the range of 0.25% (ZJ-24 and ZJ-25) to 1.16% (ZJ-26). The Chinese jujube genotypes of this study were grouped based on hierarchical clustering of the fruit, stone and seed characteristics. © 2014 Potravinarstvo. All rights reserved.


Brindza J.,Slovak University of Agriculutre in Nitra | Kucelova L.,Slovak University of Agriculutre in Nitra | Sinica A.,Slovak University of Agriculutre in Nitra | Stehlikov B.,Slovak University of Agriculutre in Nitra | Culakova M.,Slovak University of Agriculutre in Nitra
Potravinarstvo | Year: 2013

This work aimed at the morphological, biochemical, technological and sensorial determination of black mulberry (Morus nigra L.-MN) fruitage and their utilization in the food production branches. For the experimental purposes were selected 50 genotypes of this population grown in the Pukanec surroundings. The medium fruitage weight determined in the selected collection ranged from 7.26 g (MN-1) to 1.42 g (MN-14), fruitage length in a range of 13.51 mm (MN-14) to 29.20 mm (MN-12) and the medium fruitage width 11.88 mm (MN-14)-21.12 mm (MN-2). The variability of the evaluated traits varried from low to high degree. Juice yield from matured fruitage achieved 62.40 %. From black mulberry fruitages 16 food products were prepared-juice mixed with cream, yoghurt and/or curd (in several proportions) and 3 confectionery products. Sensorial analyses showed significant differences among tested products. In the group of confectionery products was generally preferred the cream-mulberry cake. High values of antioxidative activity has been measured in the chocolate cake with a mulberry jam (36.90-28.43 %), followed by the cream-mulberry cake (29.78-12.71 %) and the fresh mulberry juice (30.97-20.17 %). The antioxidation activities exerted generally higher values with the samples tested in water, when compared with those prepared in ethanol extract. Based on the gained results 4 genotypes were selected and recommended for the use in practice, as these provided relative high values of tested traits. © 2013 Potravinarstvo.

Loading Slovak University of Agriculutre in Nitra collaborators
Loading Slovak University of Agriculutre in Nitra collaborators