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Rāhuri, India

Nikhil B.S.K.,SLBTC | Jadhav A.S.,SLBTC | Kute N.S.,SLBTC
Ecology, Environment and Conservation | Year: 2015

A polymerase chain reaction (PCR) based approach, namely inter simple sequence repeat (ISSR) analysis was applied to 22 genotypes of onion (Allium cepa L.) in order to assess the degree of polymorphism within the genes and to investigate if this approach was suitable for genetic studies of onion. For this study, twenty-two onion genotypes were evaluated for variability using a set of 15 ISSR primers. The polymorphisms in PCR amplification products were subjected to the unweighed pair group method for arithmetic averages (UPGMA). The dendogram constructed from the similarity data grouped the genotypes into different clusters. Considering all the amplified primers a total of 81 scorable amplification products were obtained out of which 69 were polymorphic bands and 12 bands were monomorphic. In this study, it was found that maximum diversity was observed between the genotypes Sel 10 and CSKO 1175. Whereas, maximum similarity was observed between the genotypes Phule Samarth and Sel 24. © Copyright EM International. Source


Nikhil B.S.K.,SLBTC | Jadhav A.S.,SLBTC | Bhalekar S.G.,SLBTC | Bhalekar M.N.,SLBTC | Kute N.S.,SLBTC
Ecology, Environment and Conservation | Year: 2015

Genetic variability, interrelationship and path coefficients were studied in onion involving 22 genotypes. Among nine traits studied highest GCV was observed for average bulb weight followed by yield and neck thickness. Heritability (broad sense) of the characters under study could be classified as high for plant height, days to maturity and average bulb weight. The genetic advance was found to be highest for average bulb weight followed by days to maturity. High heritability coupled with high genetic advance observed for days to maturity and average bulb weight. Yield was significantly and positively correlated with equatorial diameter followed by polar diameter, average bulb weight, TSS, and plant height. It could be inferred that, selection for high yield would be effective through selection for these traits. The path coefficient analysis revealed thatpolar diameter followed by average bulb weight and days to maturity had positive direct effect on yield. This indicates the true relationship of these traits and a direct selection through these traits will be effective. © Copyright EM International. Source

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